Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	101,802	A→C	8.3%	K208Q (AAG→CAG) ‡	prlC →	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	101,802	0	A	C	8.3%	215.2 / 9.7	72	K208Q (AAG→CAG) ‡	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base A (30/36);  new base C (3/3);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

AGAGCAAGCTCAGCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGCCAGTTCGA  >  NC_002947/101656‑101950                                                                                                                                                   |                                                                                                                                                     agagCAAGCTCAGCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAg                                                                                                                                                    <  6:28745/149‑1 (MQ=255)        gCTCAGCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTgg                                                                                                                                             <  4:289936/149‑1 (MQ=255)          tCAGCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGAc                                                                                                                                           <  1:140280/149‑1 (MQ=255)          tCAGCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGAc                                                                                                                                           >  6:168072/1‑149 (MQ=255)            aGCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCCTTGGCCTgggct                                                                                                                                          <  7:68352/148‑4 (MQ=255)             gCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGc                                                                                                                                        <  7:90203/149‑1 (MQ=255)               gAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGTCCAGGCCCTTGGCCTgggctgcag                                                                                                                                      <  7:171051/149‑8 (MQ=255)                 gCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGt                                                                                                                                    <  5:347969/149‑1 (MQ=255)                    gggCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGCCCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGa                                                                                                                                 >  6:79029/1‑149 (MQ=255)                    gggCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGa                                                                                                                                 <  1:151651/149‑1 (MQ=255)                     ggCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGtt                                                                                                                                   <  3:304774/146‑1 (MQ=255)                     ggCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGtt                                                                                                                                   >  4:304774/1‑146 (MQ=255)                     ggCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGAt                                                                                                                                <  5:15684/149‑1 (MQ=255)                       cAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCa                                                                                                                              <  2:140822/149‑1 (MQ=255)                          ccGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCAccc                                                                                                                           >  1:126704/1‑149 (MQ=255)                           cGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCt                                                                                                                          <  5:168072/149‑1 (MQ=255)                                caccAATCAGCTGCTGGACGCCCCCCCGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATGCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGaa                                                                                                                      <  1:331032/146‑1 (MQ=255)                                 tcCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCCTTGGCCTggg                                                                                                                                            >  4:362075/1‑124 (MQ=255)                                 tcCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCCTTGGCCTggg                                                                                                                                            <  3:362075/125‑2 (MQ=255)                                 tcCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAAtt                                                                                                                    >  7:131019/1‑149 (MQ=255)                                 tcCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAAtt                                                                                                                    >  7:87146/1‑149 (MQ=255)                                   cAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAg                                                                                                                                                    <  4:66567/115‑1 (MQ=255)                                   cAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAg                                                                                                                                                    >  3:66567/1‑115 (MQ=255)                                   cAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTcc                                                                                                                  >  2:218509/1‑149 (MQ=255)                                    aaTCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTccc                                                                                                                 >  5:334352/1‑149 (MQ=255)                                      tCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCa                                                                                                               <  4:285555/149‑1 (MQ=255)                                         gctgctGGACGCCACCCTGGCCTGCACCAAGCTGGTTACCGACCAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGct                                                                                                            <  8:131019/149‑1 (MQ=255)                                            gctgGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGctac                                                                                                          >  7:348330/1‑148 (MQ=255)                                              tgGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCtactac                                                                                                       >  3:207293/1‑149 (MQ=255)                                                gACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTAcg                                                                                                      <  1:218509/148‑1 (MQ=255)                                                   gCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGt                                                                                                  <  8:87146/149‑1 (MQ=255)                                                     cACCCCGGCCTGGCCCACGCATGTTACCCACGAAGCTGCGCTGGCCGGCCGGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGtga                                                                                                <  6:195130/149‑1 (MQ=255)                                                      ccccAGGCCTGGCCCAAGCATGTTACCGACGAAGCGGCGCGGGCCGGCCTGACCGATGCGGCCACGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGtgat                                                                                               <  8:348330/148‑1 (MQ=255)                                                      aCCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGtga                                                                                                >  4:180649/1‑148 (MQ=255)                                                      aCCCAGGCCTGGACAAAGCATGTTACCGACCAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGAGGtgat                                                                                               >  1:31451/1‑149 (MQ=255)                                                        ccAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGtgatga                                                                                             >  2:9311/1‑149 (MQ=255)                                                             ccTGGACCATGCTTTTTCCCGACGATGGGGCGCTGGCGGGCCTGACGGATTGGGCCATGGCGCTGTTGGCCGGTGCCGCCCCGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTAc                                                                                        <  1:155264/149‑1 (MQ=255)                                                              cTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGGGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACg                                                                                       <  3:180649/149‑1 (MQ=255)                                                              cTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCAGGACGGCTGGTTGATCAACCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACg                                                                                       >  7:171215/1‑149 (MQ=255)                                                                  aCCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGTTGATGACCTACGCCAg                                                                                   >  7:156884/1‑149 (MQ=255)                                                                    cAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGtgat                                                                                               <  4:257871/135‑1 (MQ=255)                                                                    cAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGtgat                                                                                               >  3:257871/1‑135 (MQ=255)                                                                       gcttGTTACCGACTAAGCTGCCTTGGCCGGCCTGACCGTTTCTGCCAAGGCGCTGTGGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGTTCACCCTTGAATTCCCCAGCTACTACGCGGGGATGACCTACGCCAGCGAcc                                                                              <  1:267316/146‑1 (MQ=255)                                                                                cgacgaAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTgc                                                                     >  6:198158/1‑149 (MQ=255)                                                                                  acgaAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTgcgc                                                                   >  2:270989/1‑149 (MQ=255)                                                                                    gaAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGa                                                                 <  7:205413/149‑1 (MQ=255)                                                                                     aaGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGaa                                                                <  6:196841/149‑1 (MQ=255)                                                                                       gCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAaga                                                              <  6:334352/149‑1 (MQ=255)                                                                                          gcgcTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCt                                                           <  8:177751/149‑1 (MQ=255)                                                                                           cgcTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCt                                                           <  2:126704/148‑1 (MQ=255)                                                                                            gcTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCtat                                                         <  8:64095/149‑1 (MQ=255)                                                                                            gcTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCta                                                          >  2:52494/1‑148 (MQ=255)                                                                                               gcctctccTGACCGCTTCGGCCAAGGCGCTCTGGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGGTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATAc                                                       <  8:62389/142‑1 (MQ=255)                                                                                                    gccTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACgccgcc                                                 <  2:3215/149‑1 (MQ=255)                                                                                                    gcattaCCGATTGGTCCAAGGAGCTGATGGCCGCTGCAGCCCAGGCAAAGGTCCTGTACGGCTTGTTGATCACCATTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACgccgcc                                                 <  4:207293/144‑1 (MQ=255)                                                                                                      cTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCCCCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATAc                                                       >  7:62389/1‑141 (MQ=255)                                                                                                         aCCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACt                                             <  1:52494/148‑1 (MQ=255)                                                                                                                    cAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCATCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCt                                 >  1:286486/1‑149 (MQ=255)                                                                                                                            aGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGTTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAg                         <  8:156884/149‑1 (MQ=255)                                                                                                                                ggCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGGGAAGAGCTATACGCCGACTACTGCACCCTCGCCTCCGACCAGGGc                      <  2:31451/148‑1 (MQ=255)                                                                                                                                 gCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGccc                    <  5:79029/149‑1 (MQ=255)                                                                                                                                  ccGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGccc                    >  4:279746/1‑148 (MQ=255)                                                                                                                                         aGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCg            >  3:168310/1‑149 (MQ=255)                                                                                                                                           tccAGGCCCTTGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACACGCGCCTCCGACCAGGGCCCGAATGCCGGc          >  8:334163/2‑149 (MQ=255)                                                                                                                                           cccAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGc                                           >  5:10047/1‑116 (MQ=255)                                                                                                                                            ccAGGCCCTTGGCCTGGGCGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGc          >  8:24290/1‑148 (MQ=255)                                                                                                                                            ccAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGc          >  5:159503/1‑148 (MQ=255)                                                                                                                                             cAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGc          >  6:224750/1‑147 (MQ=255)                                                                                                                                             cAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGCCa        >  8:107175/1‑149 (MQ=255)                                                                                                                                              aGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGCCAg       >  4:26008/1‑149 (MQ=255)                                                                                                                                                gCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGGCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGCCAGtt     <  1:270989/149‑1 (MQ=255)                                                                                                                                                  cAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGCCCA‑GGCCCGAATGCCGGCCAGTTCGa  >  7:182752/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     AGAGCAAGCTCAGCGAGCTGGGCAGCCGTTTCTCCAATCAGCTGCTGGACGCCACCCAGGCCTGGACCAAGCATGTTACCGACGAAGCTGCGCTGGCCGGCCTGACCGATTCGGCCAAGGCGCAGATGGCCGCTGCAGCCCAGGCCAAGGGCCTGGACGGCTGGTTGATCACCCTTGAATTCCCCAGCTACTACGCGGTGATGACCTACGCCAGCGACCGCGCCCTGCGCGAAGAGCTATACGCCGCCTACTGCACCCGCGCCTCCGACCAGGGCCCGAATGCCGGCCAGTTCGA  >  NC_002947/101656‑101950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑