Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,371,052 G→T 7.7% G249V (GGC→GTC)  PP_3847 → LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,371,0520GT 7.7% 192.9 / 2.5 68G249V (GGC→GTC) PP_3847LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (34/28);  new base T (3/3);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAGTTGGATACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAATGTTGGC  >  NC_002947/4370904‑4371196
                                                                                                                                                    |                                                                                                                                                
cagcagTTGGATACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCgg                                                                                                                                                  >  7:77913/1‑149 (MQ=255)
  gcagTTGGATACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGATATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCa                                                                                                                                                <  3:308540/149‑1 (MQ=255)
     gTTGGATACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTa                                                                                                                                             <  2:330219/149‑1 (MQ=255)
        ggATACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCgg                                                                                                                                                  >  7:133313/1‑141 (MQ=255)
        ggATACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCgg                                                                                                                                                  <  8:133313/141‑1 (MQ=255)
          aTACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAAtct                                                                                                                                        >  7:208678/1‑149 (MQ=255)
              gccgcTTTCCGACCGTGAACTGGAAGTTGGCCGGATTATGCTTAGCTGCTTCTATAACAAGGAGATCTCGCGCAAGCTGGCGATTTCCTCAGAGACTGTTAATGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGt                                                                                                                                    <  6:85749/149‑1 (MQ=255)
                cgcTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTcc                                                                                                                                  >  1:346025/1‑149 (MQ=255)
                cgcTTTACGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTcc                                                                                                                                  >  3:251525/1‑149 (MQ=255)
                   tttCCGTCCGTGAACTGGTAGTTTGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAAtctaa                                                                                                                                      <  8:228250/142‑3 (MQ=255)
                   tttCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGATCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTAAGAAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTAAgagatcg                                                                                                                               >  7:228250/1‑143 (MQ=255)
                        gCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCTGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGtt                                                                                                                          <  3:295728/149‑1 (MQ=255)
                          ccGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCCCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGTCATTAAATCTCAGTCCGAGCTGTtc                                                                                                                         >  6:237698/1‑148 (MQ=255)
                                 cTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCattatt                                                                                                                 >  5:368042/1‑149 (MQ=255)
                                    gAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGATCAATGAGATCGCGCTCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCTCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTAtttt                                                                                                               <  3:136156/148‑1 (MQ=255)
                                    gAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTAttttt                                                                                                              >  7:103333/1‑149 (MQ=255)
                                           gCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTtctc                                                                                                                       >  8:139917/1‑133 (MQ=255)
                                           gCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTtctc                                                                                                                       <  7:139917/133‑1 (MQ=255)
                                             cGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGca                                                                                                       >  5:130437/1‑147 (MQ=255)
                                                 ttATGCTTAGCGGCTGCTCTAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTAAAGCAAACTAGGCATTAAATCTCAGTACGAGCTGGTCTCATTATTTTTGAACGCACAgcac                                                                                                 >  8:327509/1‑149 (MQ=255)
                                                   aTGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAgaact                                                                                                <  8:24641/148‑1 (MQ=255)
                                                           cGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGtt                                                                                       <  6:130437/149‑1 (MQ=255)
                                                                 ctCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTtct                                                                                   <  2:100181/147‑1 (MQ=255)
                                                                 ctCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTtct                                                                                   <  8:208678/147‑1 (MQ=255)
                                                                  tCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACTCACAGAACTGAACTCGTTTTCTCat                                                                                >  4:341474/1‑149 (MQ=255)
                                                                  tCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAgaactga                                                                                              >  4:19908/1‑135 (MQ=255)
                                                                  tCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAgaactga                                                                                              <  3:19908/135‑1 (MQ=255)
                                                                  tCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAAGTCGttttgtgat                                                                                >  6:89603/1‑144 (MQ=255)
                                                                      aaaaGGAGATATCGCGCAAGCTTGCTATTTCCTCAGATAATTTGAAGGTCCATAGCAAGCAGATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCatga                                                                              <  5:376504/145‑1 (MQ=255)
                                                                       cAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATCATgg                                                                           <  8:77913/149‑1 (MQ=255)
                                                                             gaTCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACACCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGATTTCTCATGACGGCTTCaa                                                                     <  4:251525/149‑1 (MQ=255)
                                                                                    cgcAATCTTGCGATTTCCGCAGAGACTGTGAAGGTCCTTCGCAAGCACATGTACAGCATACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGTACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTaa                                                              <  8:103333/149‑1 (MQ=255)
                                                                                      cAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGa                                                            <  1:36430/149‑1 (MQ=255)
                                                                                           tGGCGATTTTCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATc                                                       >  4:300046/1‑149 (MQ=255)
                                                                                             gCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCgg                                                     >  7:143336/1‑149 (MQ=255)
                                                                                             gCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGAATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGATTCAAGTTATAATCCAATCgg                                                     >  8:140016/1‑149 (MQ=255)
                                                                                               gATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGTCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGtc                                                   <  5:237698/149‑1 (MQ=255)
                                                                                               gATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGa                                                            <  1:308591/140‑1 (MQ=255)
                                                                                               gATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGa                                                            >  2:308591/1‑140 (MQ=255)
                                                                                                 tttCCGCAGAGACTGTGAAGGTCCATCGCAAGCCCATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTAATTATTTTTGAACGCACAGAACTGAACACGTTTACTCATGATGGCTTCAACTTCTAAGACAAACGGtctc                                                 >  7:94317/1‑149 (MQ=255)
                                                                                                     cGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTg                                             >  6:102429/1‑149 (MQ=255)
                                                                                                     cGCAGAGACTGTGAAGGACCATCGCAAGCACATGAACAGCAAACTCGTCATAAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGtt                                              <  2:346025/148‑1 (MQ=255)
                                                                                                        agagACTGTGAATGTCCATCTCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCgg                                          <  7:140016/149‑1 (MQ=255)
                                                                                                        agagACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCgg                                          <  5:102429/149‑1 (MQ=255)
                                                                                                           gaCTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCggg                                         >  6:311982/1‑147 (MQ=255)
                                                                                                            aCTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAg                                      >  5:192089/1‑149 (MQ=255)
                                                                                                              tgtgAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTa                                    >  5:92082/1‑149 (MQ=255)
                                                                                                                 gAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTc                                 >  7:292811/1‑149 (MQ=255)
                                                                                                                     gTCCATCGCAATCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTTAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCAcc                             <  7:327509/149‑1 (MQ=255)
                                                                                                                     gTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTACAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCAcc                             <  5:89603/149‑1 (MQ=255)
                                                                                                                      tCCATCGCAAGCACATGTACAGCAAACTCGTCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCAcc                             >  8:290481/1‑148 (MQ=255)
                                                                                                                       ccATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCAcc                             >  4:88115/1‑147 (MQ=255)
                                                                                                                        cATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCa                                       >  7:51166/1‑136 (MQ=255)
                                                                                                                        cATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCa                                       <  8:51166/136‑1 (MQ=255)
                                                                                                                         aTCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGc                         >  4:192815/1‑149 (MQ=255)
                                                                                                                              aaGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCcggcg                    >  8:197324/1‑149 (MQ=255)
                                                                                                                              aaGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCcggcg                    >  8:197577/1‑149 (MQ=255)
                                                                                                                                gCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCggcggc                  >  8:331336/1‑149 (MQ=255)
                                                                                                                                    aTGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCAcc                             >  8:243557/1‑134 (MQ=255)
                                                                                                                                    aTGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCAcc                             <  7:243557/134‑1 (MQ=255)
                                                                                                                                    aTGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCaa              >  6:140935/1‑149 (MQ=255)
                                                                                                                                    aTGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCaa              >  3:90533/1‑149 (MQ=255)
                                                                                                                                         cAGCAAACTCGTCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCaa         <  7:290481/149‑1 (MQ=255)
                                                                                                                                         cAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCaa         <  7:197324/149‑1 (MQ=255)
                                                                                                                                          aGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAAt        <  3:192815/149‑1 (MQ=255)
                                                                                                                                           gCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAAGTCGTTTACACATGATGGCTTCAAGTTATAAGCCAATCGGTCTCGTTGCGGGCAGTTTTCCCCCGAGCCGGCGGCGCAAGGCAATg       >  2:284812/1‑149 (MQ=255)
                                                                                                                                            cAAACTCGTCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAATGt      >  2:30504/1‑149 (MQ=255)
                                                                                                                                              aaCTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAATGTTg    <  3:341474/149‑1 (MQ=255)
                                                                                                                                                cTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAATGTTGGc  <  5:140935/149‑1 (MQ=255)
                                                                                                                                                cTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAATGTTGGc  >  1:270591/1‑149 (MQ=255)
                                                                                                                                                 tCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAATGTTGGc  >  6:9638/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAGCAGTTGGATACGCCGCTTTCCGCCCGTGAACTGGAAGTTGGCCGGCTTATGCTTAGCGGCTGCTCGAACAAGGAGATCGCGCGCAAGCTGGCGATTTCCGCAGAGACTGTGAAGGTCCATCGCAAGCACATGTACAGCAAACTCGGCATTAAATCTCAGTCCGAGCTGTTCTCATTATTTTTGAACGCACAGAACTGAACTCGTTTTCTCATGATGGCTTCAAGTTCTAAGACAATCGGTCTCGTTGCGGGCAGTATTCCACCGAGCCGGCGGCGCAACTCAATGTTGGC  >  NC_002947/4370904‑4371196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑