Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,399,517 G→A 6.6% L125F (CTC→TTC)  PP_3878 ← minor capsid protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,399,5170GA 6.6% 273.1 / 6.3 91L125F (CTC→TTC) PP_3878minor capsid protein C
Reads supporting (aligned to +/- strand):  ref base G (38/47);  new base A (3/3);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

CAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGG  >  NC_002947/4399379‑4399665
                                                                                                                                          |                                                                                                                                                    
cAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATgtgt                                                                                                                                            <  4:357045/149‑1 (MQ=255)
 aGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGtt                                                                                                                                           >  4:315536/1‑149 (MQ=255)
  gACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTc                                                                                                                                          >  8:318912/1‑149 (MQ=255)
  gACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTc                                                                                                                                          >  7:53772/1‑149 (MQ=255)
       aaTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCa                                                                                                                                     >  7:179718/1‑149 (MQ=255)
        aTCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAACACATTGTGCTCGAcatt                                                                                                                                    <  3:174455/149‑4 (MQ=255)
         tCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATg                                                                                                                                   <  3:136748/149‑1 (MQ=255)
         tCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATg                                                                                                                                   <  1:350728/149‑1 (MQ=255)
         tCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATg                                                                                                                                   <  7:255451/149‑1 (MQ=255)
                aCCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCa                                                                                                                             <  3:315536/148‑1 (MQ=255)
                 cccccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGt                                                                                                                           <  2:134253/149‑1 (MQ=255)
                  ccccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGt                                                                                                                           >  7:82961/1‑148 (MQ=255)
                   cccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGa                                                                                                                         >  3:211907/1‑149 (MQ=255)
                   cccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGa                                                                                                                         >  1:50078/1‑149 (MQ=255)
                   cccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGa                                                                                                                         >  2:167239/1‑149 (MQ=255)
                    ccTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAACACATTGTGCTCGAc                                                                                                                                       <  3:248659/134‑1 (MQ=255)
                    ccTGAGTACGCCATGAAGTTAACCAGGCCGGGGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAACACATTGTGCTCGAc                                                                                                                                       >  4:248659/1‑134 (MQ=255)
                     cTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATg                                                                                                                       <  5:326749/149‑1 (MQ=255)
                     cTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATg                                                                                                                       >  6:362954/1‑149 (MQ=255)
                     cTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATg                                                                                                                       >  5:51324/1‑149 (MQ=255)
                        aGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGc                                                                                                                     <  4:211907/148‑1 (MQ=255)
                          tACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATATTGGTAGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTAAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCt                                                                                                                   >  1:39578/1‑148 (MQ=255)
                           aCGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTa                                                                                                                 >  3:267258/1‑149 (MQ=255)
                             gCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAtt                                                                                                               <  1:167239/149‑1 (MQ=255)
                              ccATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTAtt                                                                                                               >  4:200699/1‑148 (MQ=255)
                               cATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCa                                                                                                             <  2:268906/149‑1 (MQ=255)
                                aTGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATgg                                                                                                                      <  7:122397/139‑1 (MQ=255)
                                aTGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATgg                                                                                                                      >  8:122397/1‑139 (MQ=255)
                                aTGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAg                                                                                                            <  7:318912/149‑1 (MQ=255)
                                 tGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGTGTCAGTGATGGCCTTATTCAgg                                                                                                           <  8:207516/149‑1 (MQ=255)
                                 tGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAgg                                                                                                           <  4:267258/149‑1 (MQ=255)
                                  gAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAggg                                                                                                          >  4:327578/1‑149 (MQ=255)
                                   aaGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAggg                                                                                                          <  2:50078/148‑1 (MQ=255)
                                     gTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGgctg                                                                                                       >  5:128434/1‑149 (MQ=255)
                                      ttAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGgctgc                                                                                                      >  7:8374/1‑149 (MQ=255)
                                          ccAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGc                                                                                                                     >  6:139347/1‑130 (MQ=255)
                                          ccAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGc                                                                                                                     <  5:139347/130‑1 (MQ=255)
                                           cAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCg                                                                                                   >  1:189807/1‑147 (MQ=255)
                                           cAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCa                                                                                                 <  1:138199/149‑1 (MQ=255)
                                               ccGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGccc                                                                                             <  2:189807/149‑1 (MQ=255)
                                                cGGTTATTTGGTTGATCTTTGTCGCGGCCCGAATGTCCGCCTCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCt                                                                                            <  8:319178/149‑1 (MQ=255)
                                                cGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCt                                                                                            <  3:327578/149‑1 (MQ=255)
                                                  gTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTc                                                                                          <  1:60496/149‑1 (MQ=255)
                                                   tGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCa                                                                                         <  6:51324/149‑1 (MQ=255)
                                                   tGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCa                                                                                         <  1:234375/149‑1 (MQ=255)
                                                         gTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTg                                                                                   >  6:117348/1‑149 (MQ=255)
                                                          tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTgg                                                                                  >  6:214972/1‑149 (MQ=255)
                                                          tttGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTgg                                                                                  >  2:317079/1‑149 (MQ=255)
                                                              aTCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCAt                                                                              >  4:359768/1‑149 (MQ=255)
                                                                cTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCGGAGGGCGCTGCCGCGTGGGCTGTCAATGTCGAGAACAATGTGTTCTACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATg                                                                             <  5:241423/148‑1 (MQ=255)
                                                                  tGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGt                                                                           <  1:317079/148‑1 (MQ=255)
                                                                   ggTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtct                                                                         >  7:269479/1‑149 (MQ=255)
                                                                    gTCGCGGCCCGAATGTCCGCCTCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtctc                                                                        <  7:286679/149‑1 (MQ=255)
                                                                    gTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtctc                                                                        <  5:45913/149‑1 (MQ=255)
                                                                     tCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCg                                                                       <  5:117348/149‑1 (MQ=255)
                                                                       gcgGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTTAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCg                                                                       >  4:64541/1‑147 (MQ=255)
                                                                        cgGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAg                                                                    <  5:214972/149‑1 (MQ=255)
                                                                            ccGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGt                                                                >  1:112343/1‑149 (MQ=255)
                                                                                    ccgccgCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCa                                                                                         >  8:223007/1‑116 (MQ=255)
                                                                                    ccgccgCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCa                                                                                         <  7:223007/116‑1 (MQ=255)
                                                                                    ccgccgCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTc                                                                                >  1:190886/1‑125 (MQ=255)
                                                                                    ccgccgCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTc                                                                                <  2:190886/125‑1 (MQ=255)
                                                                                      gccgccAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCt                                                      >  6:294821/1‑149 (MQ=255)
                                                                                                 aacaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTc                                            <  2:243451/145‑1 (MQ=255)
                                                                                                  aaaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTca                                           >  7:187035/1‑148 (MQ=255)
                                                                                                   aaGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTcac                                          >  7:162549/1‑148 (MQ=255)
                                                                                                    aGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACaa                                        <  8:269479/149‑1 (MQ=255)
                                                                                                    aGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACaa                                        <  6:128434/149‑1 (MQ=255)
                                                                                                      cgcCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGa                                      <  3:200699/149‑1 (MQ=255)
                                                                                                         cGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACg                                   >  6:80917/1‑149 (MQ=255)
                                                                                                               cgcTGCCGCCTGGTCTTTCAATTTCTAGCACAATGTGTTCGACCATGGGTTCAGTGATGGCCTTATTCATGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATgg                             <  8:53772/149‑1 (MQ=255)
                                                                                                                    ccgccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTGGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACggg                        <  2:39578/149‑1 (MQ=255)
                                                                                                                      gccTGGGCTGTCATTGTCGAGCACTTTGTGTTCGACCTTGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCTTGGTCTCGCTGGCGTTCATGTGGCTGCCGCGACTCACAAGAACGCCATTGACGGGGa                      <  8:53449/149‑1 (MQ=255)
                                                                                                                      gccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtc                                                                          <  2:270893/97‑1 (MQ=255)
                                                                                                                      gccTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGtc                                                                          >  1:270893/1‑97 (MQ=255)
                                                                                                                           ggCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCAc                  <  5:294821/148‑1 (MQ=255)
                                                                                                                             cTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTc               <  8:8374/149‑1 (MQ=255)
                                                                                                                             cTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTc               <  5:362954/149‑1 (MQ=255)
                                                                                                                              tGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTc               >  2:73505/1‑148 (MQ=255)
                                                                                                                                tCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCca             <  5:80917/148‑1 (MQ=255)
                                                                                                                                  aaTGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCcgc                                                 <  6:248543/110‑1 (MQ=255)
                                                                                                                                  aaTGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCcgc                                                 >  5:248543/1‑110 (MQ=255)
                                                                                                                                    tGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACAccc        <  1:148078/149‑1 (MQ=255)
                                                                                                                                     gTCGAACACATTGTGCTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCt                <  2:40610/140‑1 (MQ=255)
                                                                                                                                     gTCGAACACATTGTGCTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCt                >  1:40610/1‑140 (MQ=255)
                                                                                                                                      tCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGt      >  5:250492/1‑149 (MQ=255)
                                                                                                                                      tCGAACACATTGTGCTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGt      >  3:129056/1‑149 (MQ=255)
                                                                                                                                        gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGa                                               <  3:171413/106‑1 (MQ=255)
                                                                                                                                        gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGa                                               >  4:171413/1‑106 (MQ=255)
                                                                                                                                        gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCt    <  3:156934/149‑1 (MQ=255)
                                                                                                                                        gAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCt    >  4:156934/1‑149 (MQ=255)
                                                                                                                                          gCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTgg  <  8:179718/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
CAGACGCAATCAGGTAACCCCCTGAGTACGCCATGAAGTTAACCAGGCCGGTGATTGGTTTGATCTTGGTCGCGGCCCGAATGTCCGCCGCCAGTTCAAAAGCGCCGACGGCGCTGCCGCCTGGGCTGTCAATGTCGAGCACAATGTGTTCGACCATGGGGTCAGTGATGGCCTTATTCAGGGCTGCTCGCAGCCCTTCATAGCTGGTCATGGTCTCGCAGGCGTTCAAGTGGCTGCCGCGACTCACAAGAACGCCATGGACGGGGATCACCTCCACACCCGTCTGG  >  NC_002947/4399379‑4399665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑