Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,560,566 T→C 6.4% Y328C (TAT→TGT)  PP_4048 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,560,5660TC 6.4% 177.5 / 2.7 62Y328C (TAT→TGT) PP_4048hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base C (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CGGCGGTGGCGGATGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGATGCCAG  >  NC_002947/4560421‑4560713
                                                                                                                                                 |                                                                                                                                                   
cggcggTGGCGGATGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATaaa                                                                                                                                                  <  3:325209/149‑1 (MQ=255)
      tGGCGGATGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCt                                                                                                                                            >  6:127979/1‑149 (MQ=255)
        gCGGATGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCtt                                                                                                                                           <  5:127979/148‑1 (MQ=255)
         cGGATGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCt                                                                                                                                         <  6:359573/149‑1 (MQ=255)
         cGGATGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCt                                                                                                                                         <  6:314935/149‑1 (MQ=255)
          ggATGGGCATTACGATAGATGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGAGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTg                                                                                                                                        >  6:171007/1‑149 (MQ=255)
             tGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGCGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTgcgc                                                                                                                                     >  8:198549/1‑149 (MQ=255)
                        aTAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCg                                                                                                                          >  2:84122/1‑149 (MQ=255)
                         tAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGt                                                                                                                         <  6:178127/149‑1 (MQ=255)
                          aGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTc                                                                                                                        >  8:68051/1‑149 (MQ=255)
                            aCGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTGCTGCGCGCGGGTCGCCGTcgc                                                                                                                      >  8:280324/1‑149 (MQ=255)
                                      ggccACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGGCGTCGCGTGATCGAac                                                                                                            <  2:289281/147‑1 (MQ=255)
                                        ccACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAacac                                                                                                          >  4:252180/1‑149 (MQ=255)
                                        ccACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAacac                                                                                                          <  3:252180/149‑1 (MQ=255)
                                        ccACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAacac                                                                                                          <  1:66881/149‑1 (MQ=255)
                                         cACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACg                                                                                                         >  4:81720/1‑149 (MQ=255)
                                                          aTTTCCAGGCGGCGAGGCCCACGCGCCGCGGACACCAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCCCCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCAt                                                                                         <  8:248374/148‑1 (MQ=255)
                                                             tCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATgcg                                                                                      >  1:230301/1‑148 (MQ=255)
                                                                          gCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGc                                                                        >  5:162451/1‑149 (MQ=255)
                                                                            ccACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGa                                                                      >  3:56384/1‑149 (MQ=255)
                                                                                 cgcAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATGACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTgg                                                                 <  5:171007/149‑1 (MQ=255)
                                                                                 cgcAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTgg                                                                 <  7:301069/149‑1 (MQ=255)
                                                                                    aGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCa                                                              >  5:108134/1‑149 (MQ=255)
                                                                                        gACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACGACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCAcggg                                                          >  6:6054/1‑149 (MQ=255)
                                                                                        gACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCAcggg                                                          >  6:173051/1‑149 (MQ=255)
                                                                                            agaGGTTTTCATGGCCTTTGCCGAGCATGTACTTATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCAcgggctgg                                                      <  4:264415/147‑1 (MQ=255)
                                                                                            acaGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCAcgggcggg                                                      <  5:6054/149‑1 (MQ=255)
                                                                                             caGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACgggcgggc                                                     >  7:98872/1‑149 (MQ=255)
                                                                                                   ttCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAgc                                                <  1:84122/148‑1 (MQ=255)
                                                                                                       tGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCAcc                                           <  7:198549/149‑1 (MQ=255)
                                                                                                       tGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCAcc                                           >  3:323459/1‑149 (MQ=255)
                                                                                                       tGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCCGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCTATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCAcc                                           >  1:339315/1‑149 (MQ=255)
                                                                                                          ccTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTc                                        <  6:108134/149‑1 (MQ=255)
                                                                                                           cTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCACAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCg                                       >  7:5618/1‑149 (MQ=255)
                                                                                                           cTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCACAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCg                                       >  7:254/1‑149 (MQ=255)
                                                                                                               gCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCAAGGGCGGGCCGAGCGCACCCTCGGAcc                                   >  5:367137/1‑149 (MQ=255)
                                                                                                                  gAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCt                                >  3:291452/1‑149 (MQ=255)
                                                                                                                      aTGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAg                            <  3:81720/149‑1 (MQ=255)
                                                                                                                      aTGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCAAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAg                            <  7:68051/149‑1 (MQ=255)
                                                                                                                        gTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAgg                           >  6:345448/1‑148 (MQ=255)
                                                                                                                            tGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAgc                                                >  6:236718/1‑123 (MQ=255)
                                                                                                                            tGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAgc                                                <  5:236718/123‑1 (MQ=255)
                                                                                                                              aTCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGAc                                    <  4:6498/133‑1 (MQ=255)
                                                                                                                              aTCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGAc                                    >  3:6498/1‑133 (MQ=255)
                                                                                                                              aTCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGt                     <  6:162451/148‑1 (MQ=255)
                                                                                                                              aTCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTg                    >  4:195494/1‑149 (MQ=255)
                                                                                                                                cACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGGCGGCGACGTGGGCCCGGGCGGGCCGAGCGCACCCTCGGCCCGCGCAAGGTAACGTggg                  >  8:348194/1‑149 (MQ=255)
                                                                                                                                 aCTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTggg                  <  8:5947/148‑1 (MQ=255)
                                                                                                                                 aCTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGgc                 <  2:230301/149‑1 (MQ=255)
                                                                                                                                 aCTGACCACCTCCCCATAAATACCCTGCTGCGCCCGGGGCGCCGTCGCTTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGgc                 <  8:234947/149‑1 (MQ=255)
                                                                                                                                  cTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGgc                 >  6:304790/1‑148 (MQ=255)
                                                                                                                                  cTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGgc                 <  5:304790/148‑1 (MQ=255)
                                                                                                                                     accaccTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCAt             <  3:195494/149‑1 (MQ=255)
                                                                                                                                      ccaccTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATg            >  7:280018/1‑149 (MQ=255)
                                                                                                                                       caccTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGAACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGc           >  7:177397/1‑149 (MQ=255)
                                                                                                                                             cccATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGAt       <  4:291452/147‑1 (MQ=255)
                                                                                                                                             cccATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGATGc     >  4:111358/1‑149 (MQ=255)
                                                                                                                                             cccACAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGAt       <  8:254/147‑1 (MQ=255)
                                                                                                                                             cccACAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGAt       <  8:5618/147‑1 (MQ=255)
                                                                                                                                              ccATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGATGc     >  7:66335/1‑148 (MQ=255)
                                                                                                                                               cATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGATGc     >  7:254511/1‑147 (MQ=255)
                                                                                                                                                aTAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGATGCCag  <  4:323459/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGGCGGTGGCGGATGGGCATTACGATAGACGCCATCCAGCCCACGCCCGGTGGGTTTCATTTCCAGGCTGCGAGGCCCACGCGCAGCGGACAACAGGTTTTCATGGCCTTTGCCGAGCATGTACTGATCACTGACCACCTCCCCATAAATACCCTTCTGCGCCCGGGTCGCCGTCGCGTGATCGAACACGTGCACATTGGCGCCATGCGCAGCGATTGTCGGCGACGTGGGCACGGGCGGGCCGAGCGCACCCTCGGACCGCTCAAGGTAACGTGGGCGCATGCCGATGCCAG  >  NC_002947/4560421‑4560713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑