Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,587,700 A→C 6.7% S39A (TCC→GCC) ‡ PP_4063 ← long‑chain fatty acid‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,587,7000AC 6.7% 189.9 / 4.7 60S39A (TCC→GCC) ‡PP_4063long‑chain fatty acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (24/32);  new base C (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCAC  >  NC_002947/4587557‑4587845
                                                                                                                                               |                                                                                                                                                  
cAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGacac                                                                                                                                                 <  3:270887/147‑1 (MQ=255)
  aGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAgg                                                                                                                                             <  5:120074/149‑1 (MQ=255)
  aGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAgg                                                                                                                                             >  2:343610/1‑149 (MQ=255)
        accacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAGGGCCtgc                                                                                                                                       <  2:54664/149‑3 (MQ=255)
        accacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCg                                                                                                                                       >  6:164287/1‑149 (MQ=255)
          cacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCt                                                                                                                                     >  6:368067/1‑149 (MQ=255)
          cacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCt                                                                                                                                     <  3:36842/149‑1 (MQ=255)
          cacTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCt                                                                                                                                     >  7:323663/1‑149 (MQ=255)
               ggCGCAGTTGGGCGACCAGATGCCAACACGTTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTc                                                                                                                                  <  7:313005/147‑1 (MQ=255)
                        gggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCgg                                                                                                                        <  1:343610/148‑1 (MQ=255)
                        gggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCgg                                                                                                                        <  8:44662/148‑1 (MQ=255)
                         ggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGc                                                                                                                      <  2:232294/149‑1 (MQ=255)
                         ggCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGc                                                                                                                      <  3:287064/149‑1 (MQ=255)
                          gCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGc                                                                                                                      >  8:323569/1‑148 (MQ=255)
                               cAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGt                                                                                                                <  5:283239/149‑1 (MQ=255)
                                       aaCACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCg                                                                                                        >  6:340216/1‑148 (MQ=255)
                                                gCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGc                                                                                              >  5:270959/1‑149 (MQ=255)
                                                          aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCt                                                                                     <  1:313816/148‑1 (MQ=255)
                                                          aCGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCt                                                                                     <  3:125779/148‑1 (MQ=255)
                                                           cGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTgg                                                                                   <  5:368067/149‑1 (MQ=255)
                                                             cccAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGTCCGATGGTCTGGGt                                                                                 <  2:28368/149‑1 (MQ=255)
                                                             cccAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGt                                                                                 <  8:323663/149‑1 (MQ=255)
                                                                aGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAg                                                                                                                                              <  6:30010/86‑1 (MQ=255)
                                                                aGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAg                                                                                                                                              >  5:30010/1‑86 (MQ=255)
                                                                aGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAg                                                                              >  7:11505/1‑149 (MQ=255)
                                                                    cGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGGGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCaa                                                                           <  3:83754/148‑1 (MQ=255)
                                                                     gATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAGGGCCtgcg                                                                                                                                      >  4:100015/1‑86 (MQ=37)
                                                                     gATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGTCCCGCCACCAGGGCCtgcg                                                                                                                                      <  3:100015/89‑4 (MQ=37)
                                                                       tCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGggt                                                                       >  4:280186/1‑149 (MQ=255)
                                                                             cgcgGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTcc                                                                 <  5:340216/149‑1 (MQ=255)
                                                                               cgGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTg                                                               <  4:327455/149‑1 (MQ=255)
                                                                                         tttCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCgg                                                     >  3:16108/1‑149 (MQ=255)
                                                                                         tttCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCgg                                                     <  7:32430/149‑1 (MQ=255)
                                                                                            cGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCACTCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCggg                                                    <  1:285749/148‑1 (MQ=255)
                                                                                             gACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGta                                                  <  8:11505/148‑1 (MQ=255)
                                                                                                  gTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTgg                                                                                   >  7:338664/1‑110 (MQ=255)
                                                                                                  gTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTgg                                                                                   <  8:338664/110‑1 (MQ=255)
                                                                                                  gTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTc                                            >  8:306430/1‑149 (MQ=255)
                                                                                                   ttCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCg                                           >  3:51216/1‑149 (MQ=255)
                                                                                                   ttCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCg                                           >  5:260039/1‑149 (MQ=255)
                                                                                                     cGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGt                                         >  5:219080/1‑149 (MQ=255)
                                                                                                        ccAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGa                                      >  4:187717/1‑149 (MQ=255)
                                                                                                         cAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGAc                                     >  7:104813/1‑149 (MQ=255)
                                                                                                           aCTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACt                                    >  7:302155/1‑148 (MQ=255)
                                                                                                             tGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCAt                                 >  3:183566/1‑149 (MQ=255)
                                                                                                               ccgccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCAt                                 <  6:274713/147‑1 (MQ=255)
                                                                                                               ccgccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATgg                               <  5:164287/149‑1 (MQ=255)
                                                                                                                  ccAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGGCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGgtg                            <  7:323569/149‑1 (MQ=255)
                                                                                                                   cAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGgtgt                           <  7:306430/149‑1 (MQ=255)
                                                                                                                             gCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTtgt                 <  3:187717/149‑1 (MQ=255)
                                                                                                                              gaGGCCCTGGTGTCCCGCCACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTtgtg                >  8:332377/2‑149 (MQ=255)
                                                                                                                                 gCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgtt             <  4:16108/149‑1 (MQ=255)
                                                                                                                                 gCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAACAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgtt             >  7:240605/1‑149 (MQ=255)
                                                                                                                                   ccTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgttgt           >  2:212962/1‑149 (MQ=255)
                                                                                                                                       gtggCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCGACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGtttt        <  6:320996/148‑1 (MQ=255)
                                                                                                                                          gCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgt              <  3:302370/139‑1 (MQ=255)
                                                                                                                                          gCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGgt              >  4:302370/1‑139 (MQ=255)
                                                                                                                                          gCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTggg     <  3:280186/148‑1 (MQ=255)
                                                                                                                                            gggACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCAc  <  6:219080/149‑1 (MQ=255)
                                                                                                                                             ggACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGgtgt                           >  4:176094/1‑123 (MQ=255)
                                                                                                                                             ggACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGgtgt                           <  3:176094/123‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CAAGATGCACCACTGGGCGCAGTTGGGCGACCAGATGCCAACACGGTCGCCGGTGTTCACGCCCAGTGCGATCAGGGCGCGGGCGTATATTTCGACCTGTTCGGCCAACTGCCGCCAGCTGTAACGCAGGCCCTGGTGGCGGGACACCAGGGCCTCGCTGTCGCAACAGCGGGCCACGGTGGCA‑TCGAAGGCCTGGCCGATGGTCTGGGTCAGCAAGGGTCGGTCCTGGCGACCGCGGGTATAGCTCGGTTGACTCATGGGTGTCCCTTCTTGTGGTTGTTTTGGGCAC  >  NC_002947/4587557‑4587845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑