Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,740,816 T→G 100% intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG99.1% 181.5 / ‑6.7 60intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (27/31);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740679‑4740954
                                                                                                                                         |                                                                                                                                          
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACTCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                <  7:307193/149‑1 (MQ=255)
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                >  3:80854/1‑149 (MQ=255)
    ccGTACAAGATCGTCCGTCCGCGACAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                            <  6:260477/149‑1 (MQ=255)
        aCAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGACCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTcgc                                                                                                                        >  4:112085/1‑149 (MQ=255)
         cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg                                                                                                                       <  8:179875/149‑1 (MQ=255)
                ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                                <  2:83884/149‑1 (MQ=255)
                       cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                         <  4:199585/149‑1 (MQ=255)
                       cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                         <  2:299619/149‑1 (MQ=255)
                        gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAag                                                                                                        >  5:374166/1‑149 (MQ=255)
                         cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                       <  2:96121/149‑1 (MQ=255)
                          gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAagag                                                                                                      <  2:138595/149‑1 (MQ=38)
                          gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                      <  5:331768/149‑1 (MQ=255)
                             aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc                                                                                                   <  3:17074/149‑1 (MQ=255)
                              gCTGTACCCCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCg                                                                                                  <  5:122543/149‑1 (MQ=255)
                              gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAAGAGGCCg                                                                                                  <  6:374166/149‑1 (MQ=38)
                              gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCg                                                                                                  <  4:80854/149‑1 (MQ=255)
                               cTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCgg                                                                                                 >  3:334820/1‑149 (MQ=255)
                                  tccacCGGCCAGCTCACGCGCGACATCGTTGCCCTGAAAGACGGCAAGTACGCTGCAGTAGCCGAACGCAAGACGTCGGCCCTGGGCCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTATTCGCGGCCTTGCCCGCTAAGTGGCCGGc                                                                                                <  2:127521/145‑1 (MQ=255)
                                  tACACCGGCGAGCAGAAGCGCGACATAGTTGCCCTGAAAGACCGCAAGTAAGATGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTGTTTATTTGTCCTGTGCCGGCATCTTCCCGTCGTTGCAAGGGCAGAGGCCGGcag                                                                                              >  8:192227/1‑148 (MQ=255)
                                    cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCATGCCCGCGAAGAGGCCGGCACAg                                                                                            >  7:165859/1‑149 (MQ=39)
                                         gCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                       <  8:351869/133‑1 (MQ=255)
                                         gCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                       >  7:351869/1‑133 (MQ=255)
                                              cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCGCGAAGAGGCCGGCACCCACACCACaa                                                                                   >  6:18702/1‑148 (MQ=255)
                                               aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCAAATCGCGGCCATGGCCGCCTAGAGGACCGCCAACACACCACaaa                                                                                  >  4:6531/1‑148 (MQ=255)
                                                gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                >  3:98966/1‑149 (MQ=255)
                                                  aGCGCGACATCGTTGCCCTGAACGACCGCAAGTAAGCTGCAGTAGCCCACCGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCgg                                                                                                 <  3:42823/130‑1 (MQ=37)
                                                  aGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATAGCGGCCATGCCCGCGAAGAGGCCgg                                                                                                 >  4:42823/1‑130 (MQ=255)
                                                   gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCt                                                                              >  4:251098/1‑148 (MQ=255)
                                                   gcgcAACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAACCCt                                                                              >  2:277531/1‑148 (MQ=255)
                                                       gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtt                                                                         <  3:251098/149‑1 (MQ=255)
                                                          atcttggCCCTAAAACCCCCCAAGTACCCTGAATAAGCCCACCGCAACACGGCGGCCCTGGGTCTG‑CTTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGttttt                                                                      <  4:334820/143‑1 (MQ=255)
                                                             gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                                   >  3:100205/1‑149 (MQ=255)
                                                             gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                                   >  5:78314/1‑149 (MQ=255)
                                                                  ccTGACAGACCGCACTTAAGCTGCAGTAGCCTAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgccc                                                              <  6:258359/149‑1 (MQ=255)
                                                                   cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACCAAACCTCCGTTTTTCTccgcccg                                                             >  5:65864/1‑149 (MQ=255)
                                                                                    aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCGCGAAGAGGCCGGCACATACACCACAAAAACTCAGTTTTTCTACGCCCGCTCACGCACCCCttt                                              >  8:201585/1‑147 (MQ=255)
                                                                                     gCTGCAGTAGCCTACCGAAAGAAGTCTGCCCTCGGTCTG‑CTTTTTTTTTTTGTCCTGTGCCGGCCGGTTCGCGGCGTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                           <  8:112679/149‑1 (MQ=255)
                                                                                     gCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTACGCCCGCTCACGCAGCCCTTTCAg                                           >  7:104421/1‑149 (MQ=255)
                                                                                         cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                       <  6:78314/149‑1 (MQ=255)
                                                                                            tacccGAACGCAAGAAGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGCCCTGTCCCGGCATATTCGCGGCTTTGCCCGCTATTAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                     <  7:134943/145‑1 (MQ=255)
                                                                                                      cAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCAt                          >  4:294913/1‑149 (MQ=255)
                                                                                                       aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGCCACCACAAAACCTCAGTTTTTCTCCGCCCGCGCACGCAGCCCTTTCAGGGTATTGAACGGCGCCTc                         >  7:11060/1‑149 (MQ=255)
                                                                                                       aaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCCCAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                         >  1:208253/4‑149 (MQ=255)
                                                                                                        agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTAACGCAGCCCATTGAGGGTATTGAACCGCCCaac                         >  4:211806/1‑146 (MQ=255)
                                                                                                         gaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCAAAAAAACTAAGTTTTTATCCGCCCGCTCACGCAGCACATTCAGGGTATTGAACGGCGCATcca                       >  8:77219/1‑149 (MQ=255)
                                                                                                          aagGCTGCCCTCGGCCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                      <  3:112085/149‑1 (MQ=255)
                                                                                                            ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca                    <  1:277531/149‑1 (MQ=255)
                                                                                                            ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTAGCGGCCATGCCCCCGAACAGGCCGGCCCAGACACCACAAAACCGCACTTTTTCTACGCCCGCGAACGCAACCCGTTCAGGGGAATGAAAGGCGCATacacca                    >  8:209711/1‑149 (MQ=255)
                                                                                                               tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttt                                                                        <  1:111687/94‑1 (MQ=37)
                                                                                                               tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCTTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGttt                                                                        >  2:111687/1‑94 (MQ=37)
                                                                                                                      gggCAG‑CTTTTTTTATTTGTCCTGGGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttg            <  5:18702/147‑1 (MQ=255)
                                                                                                                       gggaagcTTTTTTTATTTGTCCTGTGCCGGCGTCTTCGCGGCGTTGCCCGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt          <  3:161836/143‑1 (MQ=255)
                                                                                                                       ggatgcgttttttttttGTCCTGG‑GCCGGCGGTTGCGCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg         <  7:209711/142‑1 (MQ=255)
                                                                                                                       ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg         <  6:118783/149‑1 (MQ=255)
                                                                                                                       ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg         <  4:100205/149‑1 (MQ=255)
                                                                                                                       ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCCAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg         <  7:201585/149‑1 (MQ=255)
                                                                                                                       ggCAGCC‑TTTTTTATTTGCCCTGTGCCGCCCTCCTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg         <  8:11060/149‑1 (MQ=255)
                                                                                                                           agccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGCGGCCGGCACAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACGCAGCCCTTTAAGGGTATTGAACGGCTCATCCCCCACGAACTTGTTGGc       >  2:273284/5‑148 (MQ=255)
                                                                                                                               ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACggggga                           >  7:31001/1‑120 (MQ=255)
                                                                                                                               ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca  >  5:304309/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740679‑4740954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑