Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,744,600 A→C 6.7% S76A (TCC→GCC)  PP_4198 ← OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,744,6000AC 6.7% 237.0 / 7.0 75S76A (TCC→GCC) PP_4198OmpA family protein
Reads supporting (aligned to +/- strand):  ref base A (35/35);  new base C (3/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GTTTGGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTG  >  NC_002947/4744456‑4744748
                                                                                                                                                |                                                                                                                                                    
gTTTGGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCg                                                                                                                                                  >  1:174627/1‑149 (MQ=255)
     gCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGc                                                                                                                                              <  6:281320/148‑1 (MQ=255)
      ccTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTcc                                                                                                                                             >  3:282716/1‑148 (MQ=255)
      ccTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGcc                                                                                                                                             >  4:137931/1‑148 (MQ=255)
       cTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGa                                                                                                                                           >  7:154997/1‑149 (MQ=255)
        tCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGAt                                                                                                                                          >  1:144634/1‑149 (MQ=255)
            gCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTa                                                                                                                                       >  6:271857/1‑148 (MQ=255)
             cAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGATGtgcg                                                                                                                                     <  1:280244/149‑4 (MQ=255)
              aaGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGCACCGGTTCCGATGtgcgg                                                                                                                                    <  4:282716/149‑5 (MQ=255)
              aaGGCCAGCGGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACt                                                                                                                                    <  2:133626/149‑1 (MQ=255)
               aGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGGTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTg                                                                                                                                   <  6:79317/149‑1 (MQ=255)
                ggCCAGCTGCAGGCGCTGGCGCTCCAGGTCGATCTTGGCCGGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGTCTGAGGTAAGCATAATGCCGCACATCGGAACCTGTGCCGATGTAACTgg                                                                                                                                  <  2:328015/149‑1 (MQ=255)
                  ccAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGAc                                                                                                                                <  7:121307/149‑1 (MQ=255)
                   cAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGa                                                                                                                                 >  8:35572/1‑147 (MQ=255)
                         cAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCt                                                                                                                         >  6:112729/1‑149 (MQ=255)
                            gcgctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCgg                                                                                                                      >  1:87655/1‑149 (MQ=255)
                            gcgctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCgg                                                                                                                      >  4:107855/1‑149 (MQ=255)
                              gctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGTAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGgcg                                                                                                                    <  8:180482/149‑1 (MQ=255)
                                      tCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAg                                                                                                            >  5:173040/1‑149 (MQ=255)
                                      tCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAg                                                                                                            >  6:241101/1‑149 (MQ=255)
                                        cAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGc                                                                                                           <  2:104224/148‑1 (MQ=255)
                                          ggTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGAc                                                                                                        <  7:35572/149‑1 (MQ=255)
                                              gAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAc                                                                                                    <  2:87655/149‑1 (MQ=255)
                                               aGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcc                                                                                                   <  2:174627/149‑1 (MQ=255)
                                                     gCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcccgccc                                                                                              <  8:154997/148‑1 (MQ=255)
                                                     gCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcccgccc                                                                                              >  7:131329/1‑148 (MQ=255)
                                                      ccTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCAcccgcccg                                                                                             >  8:336432/1‑148 (MQ=255)
                                                          gcgcTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtc                                                                                         <  2:255852/148‑1 (MQ=255)
                                                           cgcTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcac                                                                                       <  3:107855/149‑1 (MQ=255)
                                                              tCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGc                                                                                                               >  6:208567/1‑122 (MQ=255)
                                                              tCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGc                                                                                                               <  5:208567/122‑1 (MQ=255)
                                                              tCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcacgtc                                                                                    <  6:173040/149‑1 (MQ=255)
                                                                cTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGt                                                                                                                                                >  6:356072/1‑87 (MQ=255)
                                                                cTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcacgtcac                                                                                  >  3:32976/1‑149 (MQ=255)
                                                                cTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcacgtc                                                                                    <  8:131329/147‑1 (MQ=255)
                                                                   gTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGGCAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCAcgcg                                                                               >  3:124905/1‑149 (MQ=255)
                                                                   gTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAtcacgtcacg                                                                                 <  4:32976/147‑1 (MQ=255)
                                                                   gTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCAcgcg                                                                               >  1:307292/1‑149 (MQ=255)
                                                                     tCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACgcggc                                                                             >  4:360188/1‑149 (MQ=255)
                                                                       aGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGgcgc                                                                           <  7:336432/149‑1 (MQ=255)
                                                                       aGCGCCAGATTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCAGATGTAACTGGACAGGCGCTCGGAGCGAGCACGCGACTCACCCGCCCGAAGCACGTCACGCGgcgc                                                                           >  8:40718/1‑149 (MQ=255)
                                                                        gcgcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGgcgcg                                                                          >  7:295797/1‑149 (MQ=255)
                                                                         cgcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAt                                                                                          >  8:311010/1‑132 (MQ=255)
                                                                         cgcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAAt                                                                                          <  7:311010/132‑1 (MQ=255)
                                                                         cgcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGgcgcgc                                                                         >  8:302546/1‑149 (MQ=255)
                                                                           ccAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTg                                                                       >  2:209335/1‑149 (MQ=255)
                                                                              gCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTgcgc                                                                    <  5:54898/149‑1 (MQ=255)
                                                                               cTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCTGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCgca                                                                   <  3:147489/149‑1 (MQ=255)
                                                                               cTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGAGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTgcg                                                                     >  5:43062/1‑147 (MQ=255)
                                                                                  ggCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGGCCGTATCACGTCACGCGGCGCGCTGCgcagc                                                                 <  5:322758/148‑1 (MQ=255)
                                                                                           gcgGGCAATCTCGATGTAGCGCTGGCTGAGGTAAGCATACTGCCGCACATCGGAACCGGTGCCTATGTAACTGGACAGGCGCTCTGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAat                                                       <  7:40718/149‑1 (MQ=255)
                                                                                            cgGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAatc                                                      <  7:302546/149‑1 (MQ=255)
                                                                                               gCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAatcatc                                                   <  5:241101/149‑1 (MQ=255)
                                                                                                 aaTCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCtt                                                 <  2:73312/149‑1 (MQ=255)
                                                                                                  aTCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCttt                                                <  2:348195/149‑1 (MQ=255)
                                                                                                    ctcGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCCCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGa                                              >  6:84837/1‑149 (MQ=255)
                                                                                                       gCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGa                                              <  5:271857/146‑1 (MQ=255)
                                                                                                           tAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCt                                        <  6:43062/148‑1 (MQ=255)
                                                                                                             gcgcTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTgg                                      >  4:121106/1‑148 (MQ=255)
                                                                                                                   gctgAGGTAAGCATAATGTCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGCCTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCCCATCGGAAGCCTCGTTGACCCCCTgg                                      >  6:339282/1‑142 (MQ=255)
                                                                                                                   gctgAGGTAAGCATAATGTCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTgg                                      <  5:339282/142‑1 (MQ=255)
                                                                                                                   gctgAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGc                                 <  2:307292/147‑1 (MQ=255)
                                                                                                                   gctgAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCCCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCaa                               <  5:84837/149‑1 (MQ=255)
                                                                                                                     tgAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACg                             >  5:41281/1‑149 (MQ=255)
                                                                                                                        ggTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTg                          <  6:41281/149‑1 (MQ=255)
                                                                                                                         gTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTgg                         >  3:152742/1‑149 (MQ=255)
                                                                                                                                 aaTGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATc                  >  2:167162/1‑148 (MQ=255)
                                                                                                                                  aTGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGCAGGCAACGGTGGCCTCATCCa                <  3:137931/149‑1 (MQ=255)
                                                                                                                                      gttacaTCGGCACCGGTTCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAgcgc            >  7:222119/4‑149 (MQ=255)
                                                                                                                                        cacaTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCgg          <  4:152742/149‑1 (MQ=255)
                                                                                                                                         acaTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCgg          >  5:7739/1‑148 (MQ=255)
                                                                                                                                           aTCGGCACCGGTTCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCAc       >  7:179525/1‑149 (MQ=255)
                                                                                                                                           aTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCAc       >  1:26632/1‑149 (MQ=255)
                                                                                                                                             cGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGTGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActg     >  1:125622/1‑149 (MQ=255)
                                                                                                                                               gAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgct   >  3:314687/1‑149 (MQ=255)
                                                                                                                                                aaCCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgctg  <  3:121106/149‑1 (MQ=255)
                                                                                                                                                aaCCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgctg  >  6:375888/1‑149 (MQ=255)
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GTTTGGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCGCACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCGGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTG  >  NC_002947/4744456‑4744748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑