| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,796,619 | A→G | 9.9% | intergenic (‑177/+41) | pvdH ← / ← PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,619 | 0 | A | G | 9.9% | 138.0 / 5.5 | 53 | intergenic (‑177/+41) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base A (19/28); new base G (2/3); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
AGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGC > NC_002947/4796473‑4796767 | aGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGc < 7:90426/149‑1 (MQ=255) ggTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTAcgggcggg < 2:244557/149‑1 (MQ=38) tGGACATGGGTAAATCCTCGCGCGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGtt < 5:243303/149‑1 (MQ=255) tGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGtt < 2:9332/149‑1 (MQ=255) ggACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGATACGGTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCTATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCAGCCCGTAGGTGCGGGtt > 7:327400/1‑148 (MQ=255) aCATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAc < 6:118775/149‑1 (MQ=255) aTGGGTATCTCCTCGAGAGCAAGGGGTTGGGCGGTTTTCGCCTTGGAAACGCTTGAGTGGGGGGAGGATTTATCGGTTGTTACGCGTGTTTATTTTTCTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAccc < 8:110674/149‑1 (MQ=21) aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc < 5:47929/149‑1 (MQ=255) tGGGTAAATCCTCGCGAGCAAGCGGATGGGCGTTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCcg < 5:21023/149‑1 (MQ=37) gggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTAGTTAAGCGATTTTATTTTTGTATGTGATGGCCTCTTAGCTGGTAAACCCGCCCGTAGGAGAGGTTTCaaactc > 3:117144/1‑144 (MQ=255) ggTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc < 8:323116/148‑1 (MQ=255) gTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCCCCcgcg > 1:140807/1‑148 (MQ=255) aaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAa‑ga < 5:290817/149‑1 (MQ=255) aTCCTCTCGAGCAATAGGATGGGAGGTTATCGCTTTGGAAAGGGTTGAGTGGGGGGTGGATTTAGGGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAa‑gag < 4:117144/149‑1 (MQ=38) cTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGc < 1:211560/148‑1 (MQ=255) cTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGGGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAA‑GAGGc < 7:320713/148‑1 (MQ=25) tCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCCCCCGCGAA‑GAGGc > 2:230176/1‑147 (MQ=255) gCAAGCGGAGGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAg < 5:20473/149‑1 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGc < 2:8749/139‑1 (MQ=255) aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGc > 1:8749/1‑139 (MQ=255) cGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGAt < 8:179652/149‑1 (MQ=255) gCGGTTATCGCTTTGGAAACGCTTGAGGGGTGGGAGGATTTAGCGGTTGTTCCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATc < 8:327400/149‑1 (MQ=255) ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCaa < 4:16622/149‑1 (MQ=255) ttATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATg > 6:201777/1‑149 (MQ=255) tCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCAGAAGGAGCGGGTTCACACGCGAA‑GAGGCCAGCACATGCGATAAACATCAATgac > 2:253172/1‑146 (MQ=255) tCGCTTTGGAAACGCTTGAGTGGGGGGAGGATGTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGccc < 1:139726/149‑1 (MQ=255) tCGCTTTGGAAACGCTTGAGTGAGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAA‑GAGGCCAg > 7:233723/1‑125 (MQ=21) gCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAACGAGGCCAGCACAGGCGATAAACATCAATGCCCa < 1:50209/149‑1 (MQ=255) gCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCa < 3:63586/148‑1 (MQ=255) cTTTGGAAACGCTTGAGGGGGTGGAGGATTTAGCGTTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGTGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGTA‑GAGGCCAGCACAGGCGATAAACATGAATGCCCAgg < 1:253172/149‑1 (MQ=39) tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGa > 5:290192/1‑149 (MQ=255) tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCCATAAACATAAATTGCCAGGa > 3:356642/1‑149 (MQ=255) aaaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCAGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACgcgc > 1:56618/1‑149 (MQ=255) aaCGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGcc > 8:182951/1‑149 (MQ=255) gggggATGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGcaa < 5:201777/148‑1 (MQ=255) ggaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACACGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGcaacaa > 1:296495/1‑148 (MQ=255) gttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCAc > 5:298412/1‑149 (MQ=255) aCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTa < 6:290192/148‑1 (MQ=255) cgcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCa < 8:252098/79‑1 (MQ=255) cgcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCa > 7:252098/1‑79 (MQ=255) cgcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACg < 7:182951/149‑1 (MQ=255) cgcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACg < 2:336973/149‑1 (MQ=255) tttttGTCTGTGCTGGCTTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCGGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCaaa < 6:30682/148‑1 (MQ=255) tttGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc < 2:56618/148‑1 (MQ=255) gTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCa < 2:296495/148‑1 (MQ=255) ctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACAcc > 7:325817/1‑149 (MQ=255) ttCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCg > 6:196703/1‑149 (MQ=255) gtgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAA‑GAGGCCAGCACAGACGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 3:293910/4‑148 (MQ=37) cccgcccgTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 3:119627/1‑149 (MQ=255) cccgcccgTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 4:164284/1‑149 (MQ=255) cgcccgTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCt < 6:298412/149‑1 (MQ=255) ccgTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGca > 4:248112/1‑149 (MQ=255) ggAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATc < 1:230176/149‑1 (MQ=255) gAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCg > 3:363014/1‑149 (MQ=255) aGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCACCTGCACAATCGc < 5:196703/149‑1 (MQ=255) | AGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAA‑GAGGCCAGCACAGGCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATCGC > NC_002947/4796473‑4796767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |