Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,804,608 G→A 7.3% R154C (CGC→TGC)  PP_4233 ← oxidoreductase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,804,6080GA 7.3% 158.9 / 2.7 55R154C (CGC→TGC) PP_4233oxidoreductase small subunit
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base A (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CAAACGAGCTGACAGCCAGCCCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGAC  >  NC_002947/4804471‑4804755
                                                                                                                                         |                                                                                                                                                   
cAAACGAGCTGACAGCCAGCCCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATgtggt                                                                                                                                          >  3:339501/1‑149 (MQ=255)
 aaaCGAGCTGACAGCCAGCCCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGCCCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGGGCAGCGGCAGATgtggt                                                                                                                                          >  6:270512/1‑148 (MQ=255)
 aaaCGAGCTGACAGCCAGCCCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATgtggtg                                                                                                                                         <  5:309420/149‑1 (MQ=255)
 aaaCGAGCTGACAGCCAGCCCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATgtggt                                                                                                                                          >  3:206383/1‑148 (MQ=255)
             agccagccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGa                                                                                                                                        >  7:214821/1‑138 (MQ=255)
             agccagccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGa                                                                                                                                        <  8:214821/138‑1 (MQ=255)
             agccagccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGc                                                                                                                             <  3:334572/149‑1 (MQ=255)
               ccagccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGCGGCTGGCCt                                                                                                                           >  8:183508/1‑149 (MQ=255)
                cagccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCAGCAGATGTGGTGACCGAGGCTGGCCTc                                                                                                                          >  4:322616/1‑149 (MQ=255)
                cagccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCAGCAGATGTGGTGACCGAGGCTGGCCTc                                                                                                                          >  6:162580/1‑149 (MQ=255)
                 agccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCACCCCTCCTTGACCAGGCCCAGATCGATGAGCACCTTCCGGGTGGCATCGTAGTAACGCACATACCCACTGCAGCGGCAGATGTGGTGACCGCGGCAGGCCTc                                                                                                                          >  3:251343/1‑148 (MQ=255)
                 agccCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGCGGCTGGCCTCg                                                                                                                         >  5:250513/1‑149 (MQ=255)
                     cGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGc                                                                                                                     >  8:290607/1‑149 (MQ=255)
                       aGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCgg                                                                                                                   <  4:339501/149‑1 (MQ=255)
                             aaCGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGtcctc                                                                                                              >  6:45449/1‑148 (MQ=255)
                                gCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACt                                                                                                          >  8:206616/1‑149 (MQ=255)
                                      gcgcTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGctc                                                                                                    <  7:290607/149‑1 (MQ=255)
                                         cTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCtcttc                                                                                                 <  3:263146/149‑1 (MQ=255)
                                         cTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCAACTTCGCtcttc                                                                                                 >  7:276249/1‑149 (MQ=255)
                                          tacgtacgAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCtcttct                                                                                                >  3:361039/1‑149 (MQ=255)
                                            cgtacgAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCttcttc                                                                                              <  3:187315/149‑1 (MQ=255)
                                                 gAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCCTCAACgg                                                                                         >  4:51851/1‑149 (MQ=255)
                                                           ttCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTgg                                                                               <  7:206616/149‑1 (MQ=255)
                                                              aaCCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGcc                                                                            >  5:65875/1‑149 (MQ=255)
                                                                cccTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGt                                                                          >  4:148864/1‑149 (MQ=255)
                                                                cccTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGt                                                                          >  5:327200/1‑149 (MQ=255)
                                                                   tcctTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCgg                                                                                                                   <  3:161420/105‑1 (MQ=255)
                                                                   tcctTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCgg                                                                                                                   >  4:161420/1‑105 (MQ=255)
                                                                    cctTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTcc                                                                      <  5:270512/149‑1 (MQ=255)
                                                                     ctTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTcc                                                                      <  5:193994/148‑1 (MQ=255)
                                                                      ttGACCAGGCCGAGATCGTTGAACACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTccac                                                                    >  7:54943/1‑149 (MQ=255)
                                                                        gACCAGGACGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTGCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTccac                                                                    <  4:251343/147‑1 (MQ=255)
                                                                         aCCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTc                                                                                                        >  8:254581/1‑110 (MQ=255)
                                                                         aCCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTc                                                                                                        <  7:254581/110‑1 (MQ=255)
                                                                         aCCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTccacca                                                                  <  4:206383/148‑1 (MQ=255)
                                                                            aGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCAc                                                              >  3:113425/1‑149 (MQ=255)
                                                                                 gagaTCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAg                                                         >  3:351089/1‑149 (MQ=255)
                                                                                   gaTCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCCTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCg                                                       <  3:51851/149‑1 (MQ=255)
                                                                                     tCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCgg                                                      <  6:327200/148‑1 (MQ=255)
                                                                                          gAGTACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGa                                                >  8:155653/1‑149 (MQ=255)
                                                                                                cTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAg                                                         <  1:322517/134‑1 (MQ=255)
                                                                                                cTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAg                                                         >  2:322517/1‑134 (MQ=255)
                                                                                                     gggTGGCATCGTAGTAACGCACATAGCCTGTGCAGCAGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGt                                     <  5:162580/149‑1 (MQ=255)
                                                                                                     gggTGGCATCGTAGTAACGCACATAGCCTGTGCAGCAGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGt                                     <  3:322616/149‑1 (MQ=255)
                                                                                                          gCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCa                                <  4:351089/149‑1 (MQ=255)
                                                                                                            aTCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAg                               >  6:297301/1‑148 (MQ=255)
                                                                                                            aTCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGt                              <  5:297301/149‑1 (MQ=255)
                                                                                                               gtagtaACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGc                           <  7:183508/149‑1 (MQ=255)
                                                                                                                        cacaTAGCCTGTGCAGCGGCAGATGTGGTGACCGCGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCACCGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGa                  <  6:250513/149‑1 (MQ=255)
                                                                                                                         acaTAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTgg                   >  4:42379/1‑147 (MQ=255)
                                                                                                                           aTAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAgg               >  2:207301/1‑149 (MQ=255)
                                                                                                                           aTAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAgg               <  7:155653/149‑1 (MQ=255)
                                                                                                                            tAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGc              <  8:54943/149‑1 (MQ=255)
                                                                                                                                     cAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGt      <  4:361039/148‑1 (MQ=255)
                                                                                                                                        cGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTcgac  >  4:278849/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
CAAACGAGCTGACAGCCAGCCCGAGGGCCAACGCCGAGGCGCTACGTACGAAGCCCATGTTCAACCCTCCTTGACCAGGCCGAGATCGTTGAGCACCTTGCGGGTGGCATCGTAGTAACGCACATAGCCTGTGCAGCGGCAGATGTGGTGACCGAGGCTGGCCTCGATGCGGTCCTCCACTTCGCTCTTCTTCAACGGCTGGCGCTGGGCCGTTTCCACCAGCACGGTAGCGGCATTGACGAAGCCTGGTGCGCAGTAGCTGCACTGGAAGGCGAACAGGTCGAC  >  NC_002947/4804471‑4804755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑