Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,904,885	G→T	5.7%	intergenic (‑52/+183)	PP_4312 ← / ← PP_4313	hypothetical protein/peptidylprolyl isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,904,885	0	G	T	5.7%	256.1 / 3.1	79	intergenic (‑52/+183)	PP_4312/PP_4313	hypothetical protein/peptidylprolyl isomerase
Reads supporting (aligned to +/- strand):  ref base G (38/36);  new base T (2/3);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCGACAGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATT  >  NC_002947/4904737‑4905029                                                                                                                                                       |                                                                                                                                                      tttCGACAGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCg                                                                                                                                                       >  4:218979/1‑149 (MQ=255) tttCGACAGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCg                                                                                                                                                       >  1:346614/1‑149 (MQ=255)   tCGACAGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGc                                                                                                                                                     >  3:179055/1‑149 (MQ=255)    cGACAGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGc                                                                                                                                                     <  5:169255/148‑1 (MQ=255)     gACAGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGc                                                                                                                                                     >  4:48609/1‑147 (MQ=255)          ccTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGc                                                                                                                                              >  5:186951/1‑149 (MQ=255)          ccTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGc                                                                                                                                              <  2:120301/149‑1 (MQ=255)              cAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAgg                                                                                                                                           <  3:276023/148‑1 (MQ=255)                ctgcCATCGCCGCGCCGTTGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGgcg                                                                                                                                        >  5:184887/3‑149 (MQ=255)                  gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgcgat                                                                                                                                      >  4:207077/1‑147 (MQ=255)                  gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgc                                                                                                                                         >  1:318733/1‑146 (MQ=255)                  gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgc                                                                                                                                         <  2:318733/146‑1 (MQ=255)                  gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCCCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGcc                                                                                                                                      >  2:68873/1‑149 (MQ=255)                   ccAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAAAGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGTGTTACCCGCCACATCGCCCTGCAACTAGCCGGgcgat                                                                                                                                      <  1:61352/149‑3 (MQ=255)                    cAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgcgatgt                                                                                                                                    <  3:207077/149‑5 (MQ=255)                    cAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCg                                                                                                                                    >  4:113171/1‑149 (MQ=255)                      aCGGCGCGGCGATGGCAGTGAGCACCAGGCCCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTg                                                                                                                                  <  1:68873/149‑1 (MQ=255)                        ggcgcggcgATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCa                                                                                                                                <  3:48609/149‑1 (MQ=255)                               cgATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATg                                                                                                                         <  4:179055/149‑1 (MQ=255)                                        tGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTg                                                                                                                <  6:184887/149‑1 (MQ=255)                                          aGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTgg                                                                                                               <  6:203862/148‑1 (MQ=255)                                           gCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGc                                                                                                              >  5:294184/1‑148 (MQ=255)                                                    tCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGtt                                                                                                                                                >  3:21365/1‑105 (MQ=255)                                                    tCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGtt                                                                                                                                                <  4:21365/105‑1 (MQ=255)                                                     cagcagCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑Agg                                                                                                 <  2:124345/149‑1 (MQ=255)                                                           caAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCaga                                                                                           <  6:186951/149‑1 (MQ=255)                                                                  cgacgacgGCGAAAGAATGTTTGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGcccc                                                                                    >  4:6808/1‑149 (MQ=255)                                                                   gacgacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGcccc                                                                                    >  2:354553/1‑148 (MQ=255)                                                                   gacgacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGcccc                                                                                    >  3:94324/1‑148 (MQ=255)                                                                      gacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAAc                                                                             <  3:218979/149‑1 (MQ=255)                                                                      gacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAAc                                                                             <  7:269635/149‑1 (MQ=255)                                                                         ggCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCAGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTGAGAGTGCCCC‑‑‑GAACACg                                                                          >  8:329365/1‑149 (MQ=255)                                                                           cGAAAGAATGTTTGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGtt                                                                        <  3:6808/149‑1 (MQ=255)                                                                           cGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGtt                                                                        <  6:200314/149‑1 (MQ=255)                                                                            gAAAGAATGTTGGCAACTTCATCAAGGCAGGGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGAAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGTTCAGAGTGCCCC‑‑‑GAACCCGtt                                                                        >  1:215388/1‑149 (MQ=255)                                                                            gAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGttt                                                                       >  2:299767/1‑149 (MQ=255)                                                                              aaGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTgg                                                                     <  1:354553/149‑1 (MQ=255)                                                                               aGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGt                                                                    <  6:294184/149‑1 (MQ=255)                                                                                       ggCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCt                                                            >  3:98668/1‑149 (MQ=255)                                                                                       ggCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGCGCCCC‑‑‑GAACCCGTTTGGTtcct                                                                <  2:279876/145‑1 (MQ=255)                                                                                       ggCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGCGCCCC‑‑‑GAACCCGTTTGGTtcct                                                                >  1:279876/1‑145 (MQ=255)                                                                                          aaCTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTc                                                                                              <  3:274953/115‑1 (MQ=255)                                                                                          aaCTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTc                                                                                              >  4:274953/1‑115 (MQ=255)                                                                                           aCTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAg                                                        >  2:285321/1‑149 (MQ=255)                                                                                                atcaAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGaa                                                   >  1:156949/1‑149 (MQ=255)                                                                                                 tcaAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAAt                                                  <  2:215388/149‑1 (MQ=255)                                                                                                   aaGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGc                                                <  3:113171/149‑1 (MQ=255)                                                                                                    aggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGcc                                               <  4:98668/149‑1 (MQ=255)                                                                                                    aggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGcc                                               >  7:246954/1‑149 (MQ=255)                                                                                                     ggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCg                                              >  5:156876/1‑149 (MQ=255)                                                                                                     ggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCg                                              >  2:81166/1‑149 (MQ=255)                                                                                                        a‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGtta                                           >  5:302030/1‑148 (MQ=255)                                                                                                              cggggggCAGAGAATATCGGTG‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAg                                      <  4:203576/144‑1 (MQ=255)                                                                                                               gtgtGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAgg                                     >  1:218316/1‑149 (MQ=255)                                                                                                               gtgtGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAg                                      <  6:156876/148‑1 (MQ=255)                                                                                                                  tgGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGc                                    >  7:97753/1‑147 (MQ=255)                                                                                                                    gCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAg                                 <  6:302030/148‑1 (MQ=255)                                                                                                                     cAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGc                               >  8:221186/1‑149 (MQ=255)                                                                                                                      agagAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGt                                                                                                                              <  5:40376/58‑1 (MQ=255)                                                                                                                      agagAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGt                                                                                                                              >  6:40376/1‑58 (MQ=255)                                                                                                                       gagaATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGcc                              >  2:231455/1‑148 (MQ=255)                                                                                                                       gagaATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGcc                              >  6:93793/1‑148 (MQ=255)                                                                                                                         gaATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAg                           <  8:97753/149‑1 (MQ=255)                                                                                                                          aaTATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAgg                          >  5:338336/1‑149 (MQ=255)                                                                                                                           atatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCCTTGCCTAgg                          <  1:118651/148‑1 (MQ=255)                                                                                                                            tatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGtt                        >  2:277399/1‑149 (MQ=255)                                                                                                                            tatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGt                         >  6:346006/1‑148 (MQ=255)                                                                                                                            tatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGt                         >  1:309361/1‑148 (MQ=255)                                                                                                                              tCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTTCAGTTAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTa                       <  8:246954/148‑1 (MQ=255)                                                                                                                              tCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGt                                                                    >  3:221840/1‑103 (MQ=255)                                                                                                                              tCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGt                                                                    <  4:221840/103‑1 (MQ=255)                                                                                                                                gggT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATTCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCCTTGCCTAgggaacg                     <  7:329365/148‑6 (MQ=255)                                                                                                                                 ggT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAgggg                   <  2:346614/149‑1 (MQ=255)                                                                                                                                  gT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGc                  >  7:113355/1‑149 (MQ=255)                                                                                                                                       aCCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTgg               <  8:113355/148‑1 (MQ=255)                                                                                                                                          cGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGgtgt           >  6:369211/1‑149 (MQ=255)                                                                                                                                              acaTCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTgcg       >  6:321394/1‑149 (MQ=255)                                                                                                                                                aTCGCCCGGCTAGTTTCAGGGCGCCCGTGCAGGGCATGCCATCCTTGGCAAAGCGTGTCTGGGTCAGAGTGCCCCCCAGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGtt          <  1:299767/149‑1 (MQ=255)                                                                                                                                                   gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTg         >  7:101157/1‑142 (MQ=255)                                                                                                                                                   gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTg         <  8:101157/142‑1 (MQ=255)                                                                                                                                                   gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAAtt  <  4:94324/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      TTTCGACAGCCTCCAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATT  >  NC_002947/4904737‑4905029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑