Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 353,067 A→G 100% intergenic (+34/+84) hisF → / ← cbcV imidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947353,0670AG100.0% 134.4 / NA 42intergenic (+34/+84)hisF/cbcVimidazole glycerol phosphate synthase subunit HisF/choline/betaine/carnitine ABC transporter ATP binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/23);  total (19/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATC  >  NC_002947/352924‑353205
                                                                                                                                                   |                                                                                                                                            
tGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCAc                                                                                                                                         >  5:104340/1‑149 (MQ=255)
      tggccgCCAGCACCGCGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGc                                                                                                                                   >  6:345884/6‑149 (MQ=39)
        cATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTc                                                                                                                                 >  8:289477/1‑149 (MQ=255)
          tGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCGTGCCCGcgaag                                                                                                                               <  3:143759/149‑5 (MQ=255)
             cAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCca                                                                                                                              >  1:324267/1‑147 (MQ=255)
              aGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                                           <  3:278070/149‑1 (MQ=255)
                     tGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑t                                                                                                                   <  7:289477/149‑1 (MQ=255)
                       cTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑Tga                                                                                                                 <  4:190132/149‑1 (MQ=255)
                         ggcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCAGGTTTGC‑t                                                                                                                   >  1:214362/1‑145 (MQ=255)
                          gcggcCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑t                                                                                                                   >  2:27394/1‑144 (MQ=255)
                              ccAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATctct                                                                                                          <  5:345884/149‑1 (MQ=255)
                               cAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCtg                                                                                                         >  2:81682/1‑149 (MQ=255)
                                aGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCtgt                                                                                                        <  5:97714/149‑1 (MQ=255)
                                    tCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCtgt                                                                                                        <  4:310735/145‑1 (MQ=255)
                                    tCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCtgt                                                                                                        >  3:310735/1‑145 (MQ=255)
                                         cACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑Cgg                                                                                               <  2:324267/148‑1 (MQ=255)
                                              tGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGt                                                                                           <  8:112220/147‑1 (MQ=255)
                                                   aGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCcgcg                                                                                    <  2:214362/149‑1 (MQ=255)
                                                        acaGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACCCACGCTCGCACAGGTTTGC‑TGAGATGACTGGTGGAG‑CGGGGGTGCCCCCGCAggt                                                                               >  7:327008/1‑148 (MQ=37)
                                                          aGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑TGGAGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAgg                                                                                >  1:115257/1‑149 (MQ=38)
                                                                ggAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAg                                                                       >  7:86765/1‑149 (MQ=255)
                                                                    gCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGc                                                                   >  6:270739/1‑149 (MQ=255)
                                                                      aatgcccTACTTGGCTGCGCGGGGTTTCGTGGTTCGCTCATCC‑TTTCCCGGGGCGG‑‑‑GCTTGCCCGCCCTGCGCGGCCCCCCCCGCCCCCAC‑CGTTTGCTTTTCATCTGTGTGGTCG‑GGGGGGTGCCCGCGAAGGGCCGCGAAGCGGccc                                                                 <  2:164126/144‑1 (MQ=255)
                                                                                     ttCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCg                                                  >  7:17016/1‑149 (MQ=255)
                                                                                       cgcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAGCCGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAAcc                                                   <  1:81682/147‑1 (MQ=255)
                                                                                        gcgcgGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACACGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGGCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTgg                                               >  3:26939/1‑149 (MQ=255)
                                                                                              gATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCCCACCCCCAGGTTTTG‑TGAGATCTCTGTGGGAG‑CGGGCGGGGCCGCGCAGGGCCCCGCAGCGGGCCCAAATGCTTGAACCCTGGTTATTg                                         >  3:129915/1‑149 (MQ=25)
                                                                                                         gCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCGTCGCGGGCCCGCCCGCGCCCACAGGTTTGC‑TGAGATCTCTGGGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAgcgc                              <  7:159076/149‑1 (MQ=39)
                                                                                                           tGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATATCTGTGGGAG‑CGGGCGTGCCCGCGCACGGCCGCGAAGCGGCCCCAAATGCTTCAACCGTGGGTCTTGCCGCGCAGCGCGt                            >  7:252683/1‑149 (MQ=38)
                                                                                                             aTCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTgg                          <  5:270739/149‑1 (MQ=255)
                                                                                                             aTCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGGTTGC‑TGAGAGCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGGAGCGGCCCCAATTGCTTCAACCGGGGTTCTTGCCGAGCAGCGCGTgg                          >  8:108013/1‑149 (MQ=38)
                                                                                                                  ttGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTTGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGag                                    >  1:73001/1‑134 (MQ=255)
                                                                                                                  ttGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGag                                    <  2:73001/134‑1 (MQ=255)
                                                                                                                    gACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGc                                        <  1:327752/128‑1 (MQ=255)
                                                                                                                      ccGTGGC‑T‑‑‑GCTTTGCTGCCCTTCGCGGGCACGCCCGCTCCCACTGGTTTGC‑TGAGATCTCTGGGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGGCGAGCAGCGCGTGGTAAAGCTCg                 <  8:327008/149‑1 (MQ=38)
                                                                                                                      ccGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg                 <  8:17016/149‑1 (MQ=255)
                                                                                                                      ccGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGAGCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCg                 <  6:104340/149‑1 (MQ=255)
                                                                                                                                   ctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑Cgg                                                                                               >  7:152656/1‑62 (MQ=255)
                                                                                                                                   ctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑Cgg                                                                                               <  8:152656/62‑1 (MQ=255)
                                                                                                                                      tGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGa       <  2:115257/147‑1 (MQ=255)
                                                                                                                                       gCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑TTATATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGt     <  4:129915/148‑1 (MQ=255)
                                                                                                                                         aGCCCGTCGCGGGCACGCCCGCTCCCACAGGTTTGC‑GGCGATCTCTGGGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATc  <  4:26939/149‑1 (MQ=38)
                                                                                                                                                   |                                                                                                                                            
TGGAAGGGCATGCCAGCGCGGTGCTGGCGGCCAGCATCTTCCACTTTGGCGAGTACACAGTGCCGGAGGCCAAGGCCTACATGGCTTCGCGCGGGATCGTCGTTCGCTGATCCATTGACCGGGGC‑T‑‑‑GCTTTGCAGCCCTTCGCAGGCACGCCCGCTCCCACAGGTTTGC‑TGAGATCTCTGTGGGAG‑CGGGCGTGCCCGCGAAGGGCCGCGAAGCGGCCCCAATTGCTTCAACCGTGGTTCTTGCCGAGCAGCGCGTGGTAAAGCTCGGTATCCCCGAGTATC  >  NC_002947/352924‑353205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑