Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,904,895	A→C	6.8%	intergenic (‑62/+173)	PP_4312 ← / ← PP_4313	hypothetical protein/peptidylprolyl isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,904,895	0	A	C	6.8%	226.4 / 6.8	74	intergenic (‑62/+173)	PP_4312/PP_4313	hypothetical protein/peptidylprolyl isomerase
Reads supporting (aligned to +/- strand):  ref base A (34/35);  new base C (2/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

CAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTAAGGTGC  >  NC_002947/4904750‑4905040                                                                                                                                                    |                                                                                                                                                       cAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAgg                                                                                                                                                      <  3:276023/148‑1 (MQ=255)   ctgcCATCGCCGCGCCGTTGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGgcg                                                                                                                                                   >  5:184887/3‑149 (MQ=255)     gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgcgat                                                                                                                                                 >  4:207077/1‑147 (MQ=255)     gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgc                                                                                                                                                    <  2:318733/146‑1 (MQ=255)     gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgc                                                                                                                                                    >  1:318733/1‑146 (MQ=255)     gcCAACGGCGCGGCGATGGCAGTGAGCACCAGGCCCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGcc                                                                                                                                                 >  2:68873/1‑149 (MQ=255)      ccAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAAAGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGTGTTACCCGCCACATCGCCCTGCAACTAGCCGGgcgat                                                                                                                                                 <  1:61352/149‑3 (MQ=255)       cAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCTGCAACTAGCCGGgcgatgt                                                                                                                                               <  3:207077/149‑5 (MQ=255)       cAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCg                                                                                                                                               >  4:113171/1‑149 (MQ=255)         aCGGCGCGGCGATGGCAGTGAGCACCAGGCCCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTg                                                                                                                                             <  1:68873/149‑1 (MQ=255)           ggcgcggcgATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCa                                                                                                                                           <  3:48609/149‑1 (MQ=255)                  cgATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATg                                                                                                                                    <  4:179055/149‑1 (MQ=255)                           tGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTg                                                                                                                           <  6:184887/149‑1 (MQ=255)                             aGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTgg                                                                                                                          <  6:203862/148‑1 (MQ=255)                              gCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGc                                                                                                                         >  5:294184/1‑148 (MQ=255)                                        cagcagCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑Agg                                                                                                            <  2:124345/149‑1 (MQ=255)                                              caAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCaga                                                                                                      <  6:186951/149‑1 (MQ=255)                                                     cgacgacgGCGAAAGAATGTTTGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGcccc                                                                                               >  4:6808/1‑149 (MQ=255)                                                      gacgacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGcccc                                                                                               >  3:94324/1‑148 (MQ=255)                                                      gacgacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGcccc                                                                                               >  2:354553/1‑148 (MQ=255)                                                         gacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAAc                                                                                        <  7:269635/149‑1 (MQ=255)                                                         gacgGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAAc                                                                                        <  3:218979/149‑1 (MQ=255)                                                            ggCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCAGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTGAGAGTGCCCC‑‑‑GAACACg                                                                                     >  8:329365/1‑149 (MQ=255)                                                              cGAAAGAATGTTTGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGtt                                                                                   <  3:6808/149‑1 (MQ=255)                                                              cGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGtt                                                                                   <  6:200314/149‑1 (MQ=255)                                                               gAAAGAATGTTGGCAACTTCATCAAGGCAGGGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGAAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGTTCAGAGTGCCCC‑‑‑GAACCCGtt                                                                                   >  1:215388/1‑149 (MQ=255)                                                               gAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGttt                                                                                  >  2:299767/1‑149 (MQ=255)                                                                 aaGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTgg                                                                                <  1:354553/149‑1 (MQ=255)                                                                  aGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGt                                                                               <  6:294184/149‑1 (MQ=255)                                                                          ggCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCt                                                                       >  3:98668/1‑149 (MQ=255)                                                                          ggCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGCGCCCC‑‑‑GAACCCGTTTGGTtcct                                                                           >  1:279876/1‑145 (MQ=255)                                                                          ggCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGCGCCCC‑‑‑GAACCCGTTTGGTtcct                                                                           <  2:279876/145‑1 (MQ=255)                                                                             aaCTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTc                                                                                                         >  4:274953/1‑115 (MQ=255)                                                                             aaCTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTc                                                                                                         <  3:274953/115‑1 (MQ=255)                                                                              aCTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAg                                                                   >  2:285321/1‑149 (MQ=255)                                                                                   atcaAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGaa                                                              >  1:156949/1‑149 (MQ=255)                                                                                    tcaAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAAt                                                             <  2:215388/149‑1 (MQ=255)                                                                                      aaGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGc                                                           <  3:113171/149‑1 (MQ=255)                                                                                       aggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGcc                                                          >  7:246954/1‑149 (MQ=255)                                                                                       aggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGcc                                                          <  4:98668/149‑1 (MQ=255)                                                                                        ggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCg                                                         >  2:81166/1‑149 (MQ=255)                                                                                        ggca‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCg                                                         >  5:156876/1‑149 (MQ=255)                                                                                           a‑ggcTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGtta                                                      >  5:302030/1‑148 (MQ=255)                                                                                                 cggggggCAGAGAATATCGGTG‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAg                                                 <  4:203576/144‑1 (MQ=255)                                                                                                  gtgtGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAgg                                                >  1:218316/1‑149 (MQ=255)                                                                                                  gtgtGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAg                                                 <  6:156876/148‑1 (MQ=255)                                                                                                     tgGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGc                                               >  7:97753/1‑147 (MQ=255)                                                                                                       gCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAg                                            <  6:302030/148‑1 (MQ=255)                                                                                                        cAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGc                                          >  8:221186/1‑149 (MQ=255)                                                                                                         agagAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGt                                                                                                                                         >  6:40376/1‑58 (MQ=255)                                                                                                         agagAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGt                                                                                                                                         <  5:40376/58‑1 (MQ=255)                                                                                                          gagaATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGcc                                         >  2:231455/1‑148 (MQ=255)                                                                                                          gagaATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGcc                                         >  6:93793/1‑148 (MQ=255)                                                                                                            gaATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAg                                      <  8:97753/149‑1 (MQ=255)                                                                                                             aaTATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAgg                                     >  5:338336/1‑149 (MQ=255)                                                                                                              atatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCCTTGCCTAgg                                     <  1:118651/148‑1 (MQ=255)                                                                                                               tatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGtt                                   >  2:277399/1‑149 (MQ=255)                                                                                                               tatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGt                                    >  1:309361/1‑148 (MQ=255)                                                                                                               tatCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGt                                    >  6:346006/1‑148 (MQ=255)                                                                                                                 tCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTTCAGTTAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTa                                  <  8:246954/148‑1 (MQ=255)                                                                                                                 tCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGt                                                                               <  4:221840/103‑1 (MQ=255)                                                                                                                 tCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGt                                                                               >  3:221840/1‑103 (MQ=255)                                                                                                                   gggT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATTCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCCTTGCCTAgggaacg                                <  7:329365/148‑6 (MQ=255)                                                                                                                    ggT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAgggg                              <  2:346614/149‑1 (MQ=255)                                                                                                                     gT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGc                             >  7:113355/1‑149 (MQ=255)                                                                                                                          aCCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTgg                          <  8:113355/148‑1 (MQ=255)                                                                                                                             cGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGgtgt                      >  6:369211/1‑149 (MQ=255)                                                                                                                                 acaTCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTgcg                  >  6:321394/1‑149 (MQ=255)                                                                                                                                   aTCGCCCGGCTAGTTTCAGGGCGCCCGTGCAGGGCATGCCATCCTTGGCAAAGCGTGTCTGGGTCAGAGTGCCCCCCAGAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGtt                     <  1:299767/149‑1 (MQ=255)                                                                                                                                      gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTg                    >  7:101157/1‑142 (MQ=255)                                                                                                                                      gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTg                    <  8:101157/142‑1 (MQ=255)                                                                                                                                      gCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAAtt             <  4:94324/149‑1 (MQ=255)                                                                                                                                            cTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGATTGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTaa       <  1:285321/149‑1 (MQ=255)                                                                                                                                                  gCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTAAGGTGc  <  4:104245/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                       CAGCGCCAACGGCGCGGCGATGGCAGTGAGCACCAGGCTCAGCAGCAAGTGACGACGACGGCGAAAGAATGTTGGCAACTTCATCAAGGCA‑GGCTCGTGTGGCAGAGAATATCGGGT‑TTACCCGCCACATCGCCCGGCTAGTTGCAGGGCGCCCGTGCAGTGAATGCCATCCTTGGCAACGCGTG‑‑AGGGTCAGAGTGCCCC‑‑‑GAACCCGTTTGGTTCCTCCCTGGAGGTGAATGCCGTTCCCTAGGCAAGGCCTAGGTTAGGGGCTGGGTGTTGCGCAATTGTGTAAGGTGC  >  NC_002947/4904750‑4905040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑