| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,986,717 | C→G | 6.9% | V14V (GTC→GTG) | flgM → | flagellin biosynthesis negative regulator FlgM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,986,717 | 0 | C | G | 6.9% | 268.5 / 6.4 | 83 | V14V (GTC→GTG) | flgM | flagellin biosynthesis negative regulator FlgM |
| Reads supporting (aligned to +/- strand): ref base C (41/36); new base G (2/4); total (43/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGGTTCGCGGGCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGCGACAAG > NC_002947/4986570‑4986861 | acGGGTTCGCGGGCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTATGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCa > 6:33492/1‑149 (MQ=255) ggTTCGCGGGCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACgg < 5:1628/149‑1 (MQ=255) cgcgGGCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGggcg < 8:28855/148‑1 (MQ=255) aaatgcattGCCGCCTGTCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGggcgg > 2:64898/8‑149 (MQ=255) gcgGGCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGCCCGTGacggacgga < 5:68346/149‑2 (MQ=255) atgcattGCCGCCTGTCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACggg > 1:10712/6‑144 (MQ=255) atgcattGCCGCCTGTCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACggg < 2:10712/139‑1 (MQ=255) gggCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGCCCGTGacggacgga < 5:73753/147‑2 (MQ=255) gCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAATCAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTgc < 5:161750/149‑1 (MQ=255) gCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTgc > 5:98843/1‑149 (MQ=255) cattGCCGCCTGTCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTgc > 6:217560/3‑149 (MQ=255) ccGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCgg < 5:217378/148‑1 (MQ=255) ggcAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACcgc < 1:64898/148‑1 (MQ=255) cAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTc < 5:217560/149‑1 (MQ=255) cAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGCCCGTGACAgacggagagtt < 6:54825/134‑7 (MQ=255) cAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGCCCGTGACAgacggagagtt > 5:54825/1‑128 (MQ=255) cATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGc < 3:17175/148‑1 (MQ=255) aTTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGggcggc > 3:83421/1‑123 (MQ=255) aTTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGggcggc < 4:83421/123‑1 (MQ=255) tttGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAgc > 4:69286/1‑149 (MQ=255) tgCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCTGCGcc > 1:325716/1‑148 (MQ=255) tAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGaaa > 8:219361/1‑148 (MQ=255) tAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGaaa > 6:139772/1‑148 (MQ=255) aaaGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGaaaaa > 3:69064/1‑149 (MQ=255) aaGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAAc < 3:69286/149‑1 (MQ=255) tCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGcagc > 1:280525/1‑147 (MQ=255) ggTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCa > 1:168521/1‑125 (MQ=255) ggTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCa < 2:168521/125‑1 (MQ=255) ttGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCCAGGCAGCGCCGAAAAACCGGCAgccagcca < 1:215781/149‑1 (MQ=255) cGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGc > 7:35167/1‑149 (MQ=255) cGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGc > 6:371498/1‑149 (MQ=255) gAAAAGAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTTAATAACTCTCCGTCCGTCTCGGGCGGCGTGCGCGGCAACTCCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAggg < 4:91799/149‑3 (MQ=255) gAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCg < 4:69064/149‑1 (MQ=255) aaCAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGt > 2:11035/1‑148 (MQ=255) aCAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTg > 1:327373/1‑148 (MQ=255) aaGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGaaa < 3:118946/149‑1 (MQ=255) aGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAAcc > 3:289346/1‑123 (MQ=255) aGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAAcc < 4:289346/123‑1 (MQ=255) gCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGa < 2:138568/148‑1 (MQ=255) gCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGa > 1:138568/1‑148 (MQ=255) aaGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGCCCGTGacggacggagag < 2:99743/84‑5 (MQ=255) aaGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGCCCGTGacggacggagag > 1:99743/1‑80 (MQ=255) cAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACAccagcgc < 5:74500/148‑1 (MQ=255) cAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACAccagcgc > 2:347665/1‑148 (MQ=255) aaaTACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGCCAccagcgcca > 4:30073/1‑149 (MQ=255) aaTACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACAccagcgccag < 2:280525/149‑1 (MQ=255) tACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACAccagcgccagcg < 5:281723/149‑1 (MQ=255) ccAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGaaa > 8:337730/1‑130 (MQ=255) ccAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGaaa < 7:337730/130‑1 (MQ=255) cAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGga > 6:263812/1‑147 (MQ=255) agagGTTTCTGATCATGGTCATCGACTTGAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGagag > 1:209133/1‑149 (MQ=255) gagGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAgg > 1:354521/1‑149 (MQ=255) gTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑CGCCGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCTGTGAAAGACACCCGCCCCAGCGGAGAGGCgg > 6:31732/1‑149 (MQ=255) tttCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGacac > 7:280532/1‑128 (MQ=255) tttCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGacac < 8:280532/128‑1 (MQ=255) gATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACAcc > 7:45986/1‑149 (MQ=255) gATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAG‑CAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCt > 6:200418/1‑149 (MQ=255) tCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCt > 8:154926/1‑148 (MQ=255) aTGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCa < 1:11035/148‑1 (MQ=255) aTGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCa < 5:33492/148‑1 (MQ=255) gTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGcc > 8:52088/1‑148 (MQ=255) tCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCaa < 5:263812/149‑1 (MQ=255) aTCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCaa > 7:42393/1‑147 (MQ=255) tCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAagag < 8:42393/149‑1 (MQ=255) cTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGcac < 7:181464/149‑1 (MQ=255) ttCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGcaca > 2:77825/1‑149 (MQ=255) cAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCgg > 6:87176/1‑125 (MQ=255) cAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCAcag < 6:98843/148‑1 (MQ=255) aGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCAcag > 3:106895/1‑147 (MQ=255) aGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCAcag > 6:219773/1‑147 (MQ=255) tCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAG‑CAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGtt < 5:200418/149‑1 (MQ=255) tttGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGc < 3:30073/149‑1 (MQ=255) aaTAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGCAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCag > 3:59907/1‑147 (MQ=255) aTAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCagaag < 2:354521/149‑1 (MQ=255) tAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCagaaga < 1:77825/149‑1 (MQ=255) tAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCagaaga < 5:371498/149‑1 (MQ=255) ctctCCGTCCGTCACGGGCTGCGTGCGCGGCAACACCGGGTCC‑GGCAGCGCCGAAACTGCGGCAGCCAGCGAGGAAGCTGTTAAAGTCACCAGCGCCAGCGGAGAGGCGGTACACGTCAGCCAAGAGTCACATCAGTTGCATAAGAtga < 2:158891/149‑3 (MQ=255) ctctCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCaga < 2:94743/143‑1 (MQ=255) ctctCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCaga > 1:94743/1‑143 (MQ=255) ctctCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCa < 2:125788/149‑1 (MQ=255) tctcCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAg > 5:46141/1‑149 (MQ=255) ctcCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGc > 8:280534/1‑149 (MQ=255) tccgtccgTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGc > 6:64847/1‑148 (MQ=255) cgtccgtcACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGc < 8:35167/146‑1 (MQ=255) cgtccgtcACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGCGa < 4:106895/148‑1 (MQ=255) gtccgtcACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGCGAc > 7:274415/1‑148 (MQ=255) gtccgtcACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGCGACa < 7:280534/149‑1 (MQ=255) cgtcACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCTGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGCGACAAg < 2:325716/148‑1 (MQ=255) | ACGGGTTCGCGGGCTTGCCGACAGGCGGCAATGCATTTGTGCCTAAAGTTTATATCGGGTTGGCCGAAAACAAGGCAAGCGTCCAAATACCCAGAGGTTTCTGATCATGGTCATCGACTTCAGTCGTTTGAATAACTCTCCGTCCGTCACGGGCGGCGTGCGCGGCAACACCGCGTCC‑GGCAGCGCCGAAAAACCGGCAGCCAGCCAGGAAGCGGTGAAAGACACCAGCGCCAGCGGAGAGGCGGTACACCTCAGCCAAGAGGCACAGCAGTTGCAGAAGATCAGCGACAAG > NC_002947/4986570‑4986861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |