Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,997,604 T→G 7.5% Q166P (CAG→CCG)  PP_4405 ← sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,997,6040TG 7.5% 235.3 / 7.3 77Q166P (CAG→CCG) PP_4405sensory box protein
Reads supporting (aligned to +/- strand):  ref base T (34/36);  new base G (4/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCATGGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCACGGAAC  >  NC_002947/4997459‑4997748
                                                                                                                                                  |                                                                                                                                                
gtccatgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGc                                                                                                                                                >  8:171035/1‑148 (MQ=255)
gtccatgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGACAACGCATCGCGTTCGGCCA‑ATTCACGGATCTCGTCCAGTTGCCGCtcc                                                                                                                                                >  8:77325/1‑146 (MQ=255)
 tccatgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCaa                                                                                                                                              >  7:253121/1‑149 (MQ=255)
   catgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAAct                                                                                                                                            <  5:168512/149‑1 (MQ=255)
   catgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAAct                                                                                                                                            >  6:19322/1‑149 (MQ=255)
     tgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgc                                                                                                                                          >  8:282153/1‑149 (MQ=255)
     tgGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgc                                                                                                                                          <  7:282153/149‑1 (MQ=255)
      ggCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgat                                                                                                                                         >  7:294010/1‑147 (MQ=255)
       gCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCGGCAActgga                                                                                                                                         <  3:241456/148‑3 (MQ=255)
        cAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCACATTCACGGTTCTCGTCCAGTTGCCGCTGCAActgctg                                                                                                                                        <  1:251364/149‑1 (MQ=255)
        cAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTgg                                                                                                                                       <  1:150260/149‑1 (MQ=255)
        cAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTgg                                                                                                                                       <  3:125262/149‑1 (MQ=255)
         aaTCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGt                                                                                                                                      >  2:154635/1‑149 (MQ=255)
              gCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTgg                                                                                                                                       >  4:75174/1‑143 (MQ=255)
              gCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTgg                                                                                                                                       <  3:75174/143‑1 (MQ=255)
               cGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCAccc                                                                                                                                <  3:350776/149‑1 (MQ=255)
                gACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCa                                                                                                                               <  1:154635/149‑1 (MQ=255)
                 aCAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAg                                                                                                                              <  7:171035/149‑1 (MQ=255)
                   aaCGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGa                                                                                                                            <  3:22515/149‑1 (MQ=255)
                    aCGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTTCAACTGCTGGTTCACCCAGGAc                                                                                                                           <  6:172212/149‑1 (MQ=255)
                    aCGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCAGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGAc                                                                                                                           <  6:228253/149‑1 (MQ=255)
                         tGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACt                                                                                                                       >  6:80137/1‑148 (MQ=255)
                           gCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCAc                                                                                                                    <  8:253121/149‑1 (MQ=255)
                           gCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCAc                                                                                                                    <  7:199432/149‑1 (MQ=255)
                           gCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCAc                                                                                                                    <  1:129597/149‑1 (MQ=255)
                           gCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCAc                                                                                                                    >  1:250968/1‑149 (MQ=255)
                             ggAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACgg                                                                                                                  <  8:54157/149‑1 (MQ=255)
                                   gcTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGt                                                                                                            >  2:341146/1‑149 (MQ=255)
                                       ggCGCGGTGGGACTGCAGCTGCAACGCGCTCTCCAGCCGGTATCGGTTGGCGAGACGGGTCAACGCATCGCGTTCGGGCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCAGCCAGGACAACTCACGGGTGCGTTCgg                                                                                                        <  7:77325/149‑1 (MQ=255)
                                         cgcgcTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGcc                                                                                                      <  5:19322/149‑1 (MQ=255)
                                           cgcTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCAc                                                                                                    <  1:341146/149‑1 (MQ=255)
                                           cgcTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCAc                                                                                                    <  2:141616/149‑1 (MQ=255)
                                               ggCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTg                                                                                                 >  8:211477/1‑148 (MQ=255)
                                                    tCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTc                                                                                           >  8:305407/1‑149 (MQ=255)
                                                      cAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCa                                                                                         <  7:305407/149‑1 (MQ=255)
                                                                  gcTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGttct                                                                             >  6:266024/1‑149 (MQ=255)
                                                                      tcCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTg                                                                         >  7:273186/1‑149 (MQ=255)
                                                                      gccAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGttc                                                                              >  2:54041/2‑144 (MQ=255)
                                                                      gccAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGttc                                                                              <  1:54041/143‑1 (MQ=255)
                                                                        cAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTc                                                                                                          <  3:107777/114‑1 (MQ=255)
                                                                        cAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTc                                                                                                          >  4:107777/1‑114 (MQ=255)
                                                                           ccGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCa                                                                    <  7:211477/149‑1 (MQ=255)
                                                                           ccGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACACACGGGTGCGTACGGCCACCGGAAACTCCAGCGACAGGTTCTTCTGTTcc                                                                     >  2:330916/1‑148 (MQ=255)
                                                                              gTAACGGTTGGCGACAAGGGTCACCGCACCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGtt                                                                 <  8:294010/149‑1 (MQ=255)
                                                                                aaCGGTTGGCGACACGGGTCAACGCATCGCGGTCGGCCA‑ATTCACGGTTCTCGTCCAGTTTCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCCACTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGtt                                                                 >  3:93686/1‑147 (MQ=255)
                                                                                   ggTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCAGCAACTGCTGGTTCACCCACGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCACCGACAGGTACAACTGTTCCACTTgagc                                                             >  7:181121/1‑147 (MQ=255)
                                                                                               gggTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTg                                                >  6:112213/1‑149 (MQ=255)
                                                                                                ggTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGttctt                                                                            <  2:174916/120‑1 (MQ=255)
                                                                                                ggTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGttctt                                                                            >  1:174916/1‑120 (MQ=255)
                                                                                                  tCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCa                                             >  7:204009/1‑149 (MQ=255)
                                                                                                       gCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTc                                        <  8:71161/149‑1 (MQ=255)
                                                                                                        cATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCg                                       <  1:330916/149‑1 (MQ=255)
                                                                                                         aTCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGa                                      >  1:200564/1‑149 (MQ=255)
                                                                                                          tCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGat                                     <  5:80137/149‑1 (MQ=255)
                                                                                                               ttCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGc                                <  2:250968/149‑1 (MQ=255)
                                                                                                               ttCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGc                                >  6:140746/1‑149 (MQ=255)
                                                                                                                  ggCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGAt                             <  8:204009/149‑1 (MQ=255)
                                                                                                                    ccA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATgc                           <  8:273186/149‑1 (MQ=255)
                                                                                                                          tCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCAcc                      >  3:334763/1‑149 (MQ=255)
                                                                                                                              ggTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAg                   >  3:166883/1‑148 (MQ=255)
                                                                                                                                ttCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACCCCAAACGCTTGCCAGCGTCGATATTGCGATGAGCACCCAGCAt                >  2:42269/1‑149 (MQ=255)
                                                                                                                                   tcGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTga                                                               >  3:290360/1‑99 (MQ=255)
                                                                                                                                   tcGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTga                                                               <  4:290360/99‑1 (MQ=255)
                                                                                                                                    ccagcAGTTGCAGCGGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGt            <  6:129792/145‑1 (MQ=255)
                                                                                                                                    ccagcAGTTGCAGCGGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGt            >  5:129792/5‑149 (MQ=255)
                                                                                                                                     gTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGaca                                                           >  7:279022/1‑101 (MQ=255)
                                                                                                                                     gTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGaca                                                           <  8:279022/101‑1 (MQ=255)
                                                                                                                                     gTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCt                                                  >  6:11725/1‑110 (MQ=255)
                                                                                                                                     gTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCt                                                  <  5:11725/110‑1 (MQ=255)
                                                                                                                                     gTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGt            <  5:112213/148‑1 (MQ=255)
                                                                                                                                     cagcAGTTGCAGCGGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTg           >  1:109950/4‑149 (MQ=255)
                                                                                                                                     cagcAGTTGCAGCGGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTg           >  4:172399/4‑149 (MQ=255)
                                                                                                                                       gcAGTTGCAGCGGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCGAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGGATGCGTGcc         >  4:292987/2‑149 (MQ=255)
                                                                                                                                         aGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCAc       >  3:339000/1‑149 (MQ=255)
                                                                                                                                           ttGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCACg      >  8:305947/1‑148 (MQ=255)
                                                                                                                                           ttGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGGTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGAAATTGCGATCCGCACCCAGCAGGCGAGCCCCg      >  4:67637/1‑148 (MQ=255)
                                                                                                                                           ttGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTACAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATTCGCAACCAGCATGCGTGCCACg      >  4:72603/1‑148 (MQ=255)
                                                                                                                                              ccGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCACGGAAc  >  1:273663/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTCCATGGCAATCAGCGACAACGGCTGGCGGAAGCGCTGGGCGCGCTGGCACTCCAGCTGCAACGCGCTCTCCAGCCGGTAACGGTTGGCGACACGGGTCAACGCATCGCGTTCGGCCA‑ATTCACGGTTCTCGTCCAGTTGCCGCTGCAACTGCTGGTTCACCCAGGACAACTCACGGGTGCGTTCGGCCACCTGAAACTCCAGCGACTGGTTCTTCTGTTCCAGTTGAGACACCAAACGCTTGCCAGCGTCGATATTGCGATGCGCACCCAGCATGCGTGCCACGGAAC  >  NC_002947/4997459‑4997748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑