Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,080,643	G→A	7.0%	A299V (GCG→GTG) ‡	lysC ←	aspartokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,080,643	0	G	A	7.0%	168.9 / 2.1	57	A299V (GCG→GTG) ‡	lysC	aspartokinase
Reads supporting (aligned to +/- strand):  ref base G (24/29);  new base A (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

GACGATCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGG  >  NC_002947/5080501‑5080788                                                                                                                                               |                                                                                                                                                  gacgaTCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGt                                                                                                                                             >  8:241151/1‑149 (MQ=255)    gaTCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCt                                                                                                                                          <  4:343042/149‑1 (MQ=255)     aTCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTg                                                                                                                                         <  3:81424/149‑1 (MQ=255)      tCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTg                                                                                                                                         >  4:271103/1‑148 (MQ=255)      tCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGc                                                                                                                                        <  2:73406/149‑1 (MQ=255)        gagaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGc                                                                                                                                        >  1:126884/1‑147 (MQ=255)        gagaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGc                                                                                                                                        <  2:126884/147‑1 (MQ=255)         agaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGtt                                                                                                                                            >  5:3903/1‑142 (MQ=255)         agaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGtt                                                                                                                                            <  6:3903/142‑1 (MQ=255)         agaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCAc                                                                                                                                      >  4:351421/1‑148 (MQ=255)             cTTGGCGATCTTGGAGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTGTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCAGAACGTTGCGCACGAtaa                                                                                                                                 <  8:315393/149‑3 (MQ=255)              ttGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCAt                                                                                                                                >  2:191997/1‑149 (MQ=255)                tgCGATCTTGGTCTCGCAGATCACTTCACGGGCACCGATTTCGCTCTCTGTGTTTTCCAGCACGCTCTGCGCCTTCTCTTACTCATTGCGGTGAACGGTGAAGGTGAAGTCGGTTTTGTTATCGTGCGCAACGTTCTGCACGATCAt                                                                                                                                <  7:128486/146‑1 (MQ=255)                ggCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCAGAACGTTGCGCACGAtaaca                                                                                                                               <  3:111354/148‑5 (MQ=255)                         ggTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACtt                                                                                                                        <  3:345787/146‑1 (MQ=255)                         ggTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCg                                                                                                                      <  3:22307/148‑1 (MQ=255)                               gCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTc                                                                                                                <  1:191997/148‑1 (MQ=255)                               gCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTc                                                                                                                >  5:127638/1‑148 (MQ=255)                                       cTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGagttatag                                                                                                       >  1:131375/1‑142 (MQ=255)                                            cGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCgg                                                                                                  <  3:289965/149‑1 (MQ=255)                                               gCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGAt                                                                                                     <  7:29613/143‑1 (MQ=255)                                               gCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGAt                                                                                                     >  8:29613/1‑143 (MQ=255)                                                 aCCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGcc                                                                                               >  3:322551/1‑147 (MQ=255)                                                     aTTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGAt                                                                                         >  8:133365/1‑149 (MQ=255)                                                     aTTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCATCTCGTACTCATTGCGGTGCACGGTCAAGGTGAAGTCGGTGGTGTTATCCTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGATCGAAGCGCTGATCGGGCAGAGGa                                                                                          >  1:165703/1‑148 (MQ=255)                                                          gcgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTg                                                                                     <  3:271103/148‑1 (MQ=255)                                                          gcgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTg                                                                                     >  8:245416/1‑148 (MQ=255)                                                          gcgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTg                                                                                     >  2:192229/1‑148 (MQ=255)                                                           cgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGaa                                                                                   <  7:241151/149‑1 (MQ=255)                                                                       tCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCg                                                                        <  3:351421/148‑1 (MQ=255)                                                                          aGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCgggg                                                                     >  6:132690/1‑148 (MQ=255)                                                                           gCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTa                                                                   >  7:258418/1‑149 (MQ=255)                                                                            cACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTAGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTAt                                                                  <  1:266123/149‑1 (MQ=255)                                                                             aCGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATc                                                                 <  3:117418/149‑1 (MQ=255)                                                                                ctctGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCgg                                                                       <  5:26448/140‑1 (MQ=255)                                                                                ctctGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCgg                                                                       >  6:26448/1‑140 (MQ=255)                                                                                 tctGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCgg                                                              >  1:201959/1‑148 (MQ=255)                                                                                   tGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCAc                                                           >  8:242220/1‑149 (MQ=255)                                                                                          tctcGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCcgcg                                                     >  4:160459/1‑148 (MQ=255)                                                                                              gTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATg                                                 <  6:127638/148‑1 (MQ=255)                                                                                              gTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATg                                                 <  7:245416/148‑1 (MQ=255)                                                                                                aCTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGt                                               >  3:18553/1‑148 (MQ=255)                                                                                                aCTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGt                                               >  7:52026/1‑148 (MQ=255)                                                                                                    aTTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGc                                           <  4:322551/148‑1 (MQ=255)                                                                                                    aTTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCt                                          >  6:94650/1‑149 (MQ=255)                                                                                                    aTTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCt                                          <  1:192229/149‑1 (MQ=255)                                                                                                          gTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCtt                                    <  3:160459/149‑1 (MQ=255)                                                                                                               cGGTGAAGGGTAAGTCGGTCGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATGGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTtcatc                                <  2:165703/148‑1 (MQ=255)                                                                                                                      ggtgaagTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGa                        <  5:352816/149‑1 (MQ=255)                                                                                                                       gtgaagTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGaa                       <  8:52026/149‑1 (MQ=255)                                                                                                                          aagTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAgg                     <  8:258418/148‑1 (MQ=255)                                                                                                                                    tgtTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGaa          <  5:94650/149‑1 (MQ=255)                                                                                                                                     gtTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCTGaaa         <  7:242220/149‑1 (MQ=255)                                                                                                                                     atgaTCGTGCAGAACGTTGCGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGaaa         >  3:64533/4‑149 (MQ=255)                                                                                                                                        aTCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGa      >  3:148044/1‑149 (MQ=255)                                                                                                                                         tCGTGCAGAACGTTGCGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGAt     >  5:57779/1‑149 (MQ=255)                                                                                                                                            tGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCgg  <  4:18553/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  GACGATCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGG  >  NC_002947/5080501‑5080788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑