Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,080,651	C→G	6.9%	Q296H (CAG→CAC)	lysC ←	aspartokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,080,651	0	C	G	6.9%	184.4 / 4.0	58	Q296H (CAG→CAC)	lysC	aspartokinase
Reads supporting (aligned to +/- strand):  ref base C (24/30);  new base G (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

GATCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATG  >  NC_002947/5080504‑5080798                                                                                                                                                    |                                                                                                                                                    gaTCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCt                                                                                                                                                    <  4:343042/149‑1 (MQ=255)  aTCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTg                                                                                                                                                   <  3:81424/149‑1 (MQ=255)   tCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTg                                                                                                                                                   >  4:271103/1‑148 (MQ=255)   tCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGc                                                                                                                                                  <  2:73406/149‑1 (MQ=255)     gagaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGc                                                                                                                                                  >  1:126884/1‑147 (MQ=255)     gagaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGc                                                                                                                                                  <  2:126884/147‑1 (MQ=255)      agaCCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCAc                                                                                                                                                >  4:351421/1‑148 (MQ=255)          cTTGGCGATCTTGGAGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTGTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCAGAACGTTGCGCACGAtaa                                                                                                                                           <  8:315393/149‑3 (MQ=255)           ttGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCAt                                                                                                                                          >  2:191997/1‑149 (MQ=255)             tgCGATCTTGGTCTCGCAGATCACTTCACGGGCACCGATTTCGCTCTCTGTGTTTTCCAGCACGCTCTGCGCCTTCTCTTACTCATTGCGGTGAACGGTGAAGGTGAAGTCGGTTTTGTTATCGTGCGCAACGTTCTGCACGATCAt                                                                                                                                          <  7:128486/146‑1 (MQ=255)             ggCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCAGAACGTTGCGCACGAtaaca                                                                                                                                         <  3:111354/148‑5 (MQ=255)                      ggTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACtt                                                                                                                                  <  3:345787/146‑1 (MQ=255)                      ggTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCg                                                                                                                                <  3:22307/148‑1 (MQ=255)                            gCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTc                                                                                                                          >  5:127638/1‑148 (MQ=255)                            gCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTc                                                                                                                          <  1:191997/148‑1 (MQ=255)                                    cTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGagttatag                                                                                                                 >  1:131375/1‑142 (MQ=255)                                         cGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCgg                                                                                                            <  3:289965/149‑1 (MQ=255)                                            gCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGAt                                                                                                               <  7:29613/143‑1 (MQ=255)                                            gCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGAt                                                                                                               >  8:29613/1‑143 (MQ=255)                                              aCCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGcc                                                                                                         >  3:322551/1‑147 (MQ=255)                                                  aTTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGAt                                                                                                   >  8:133365/1‑149 (MQ=255)                                                  aTTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCATCTCGTACTCATTGCGGTGCACGGTCAAGGTGAAGTCGGTGGTGTTATCCTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGATCGAAGCGCTGATCGGGCAGAGGa                                                                                                    >  1:165703/1‑148 (MQ=255)                                                       gcgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTg                                                                                               >  2:192229/1‑148 (MQ=255)                                                       gcgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTg                                                                                               >  8:245416/1‑148 (MQ=255)                                                       gcgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTg                                                                                               <  3:271103/148‑1 (MQ=255)                                                        cgcgcgGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGaa                                                                                             <  7:241151/149‑1 (MQ=255)                                                                    tCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCg                                                                                  <  3:351421/148‑1 (MQ=255)                                                                       aGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCgggg                                                                               >  6:132690/1‑148 (MQ=255)                                                                        gCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTa                                                                             >  7:258418/1‑149 (MQ=255)                                                                         cACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTAGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTAt                                                                            <  1:266123/149‑1 (MQ=255)                                                                          aCGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATc                                                                           <  3:117418/149‑1 (MQ=255)                                                                             ctctGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCgg                                                                                 >  6:26448/1‑140 (MQ=255)                                                                             ctctGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCgg                                                                                 <  5:26448/140‑1 (MQ=255)                                                                              tctGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCgg                                                                        >  1:201959/1‑148 (MQ=255)                                                                                tGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCAc                                                                     >  8:242220/1‑149 (MQ=255)                                                                                       tctcGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCcgcg                                                               >  4:160459/1‑148 (MQ=255)                                                                                           gTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATg                                                           <  6:127638/148‑1 (MQ=255)                                                                                           gTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATg                                                           <  7:245416/148‑1 (MQ=255)                                                                                             aCTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGt                                                         >  7:52026/1‑148 (MQ=255)                                                                                             aCTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGt                                                         >  3:18553/1‑148 (MQ=255)                                                                                                 aTTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGc                                                     <  4:322551/148‑1 (MQ=255)                                                                                                 aTTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCt                                                    <  1:192229/149‑1 (MQ=255)                                                                                                 aTTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCt                                                    >  6:94650/1‑149 (MQ=255)                                                                                                       gTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCtt                                              <  3:160459/149‑1 (MQ=255)                                                                                                            cGGTGAAGGGTAAGTCGGTCGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATGGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTtcatc                                          <  2:165703/148‑1 (MQ=255)                                                                                                                   ggtgaagTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGa                                  <  5:352816/149‑1 (MQ=255)                                                                                                                    gtgaagTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGaa                                 <  8:52026/149‑1 (MQ=255)                                                                                                                       aagTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAgg                               <  8:258418/148‑1 (MQ=255)                                                                                                                                 tgtTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGaa                    <  5:94650/149‑1 (MQ=255)                                                                                                                                  gtTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCTGaaa                   <  7:242220/149‑1 (MQ=255)                                                                                                                                  atgaTCGTGCAGAACGTTGCGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGaaa                   >  3:64533/4‑149 (MQ=255)                                                                                                                                     aTCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGa                >  3:148044/1‑149 (MQ=255)                                                                                                                                      tCGTGCAGAACGTTGCGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGAt               >  5:57779/1‑149 (MQ=255)                                                                                                                                         tGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCgg            <  4:18553/149‑1 (MQ=255)                                                                                                                                             cAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGt        >  2:92751/1‑149 (MQ=255)                                                                                                                                              aaCGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAgg                               <  2:196536/125‑1 (MQ=255)                                                                                                                                              aaCGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAgg                               >  1:196536/1‑125 (MQ=255)                                                                                                                                                  ttCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCAt   <  4:148044/149‑1 (MQ=255)                                                                                                                                                    cTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATg  <  5:132690/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GATCGAGACCTTGGCGATCTTGGTGTCGCCGATCACTTCACGGGCACCGATTTCGCGCGCGGTGTTTTCCAGCACGCTCTGCGCCTTCTCGTACTCATTGCGGTGCACGGTGAAGGTGAAGTCGGTGGTGTTATCGTGCGCAACGTTCTGCACGATCATGTCGACTTCGATGTTCGAAGCGCTGATCGGGCCGAGGATCTTGAAGGCCACGCCCGGGGTATCCGGCACGCCGCGAATGGTCAGCTTTGCTTCATCACGGTTGAAGGCGATACCGGAAATGATCGGCTGTTCCATG  >  NC_002947/5080504‑5080798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑