Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,082,883	G→T	6.9%	A461A (GCC→GCA) ‡	alaS ←	alanine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,082,883	0	G	T	6.9%	233.0 / 5.8	72	A461A (GCC→GCA) ‡	alaS	alanine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base T (2/3);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

CAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCA  >  NC_002947/5082736‑5083030                                                                                                                                                    |                                                                                                                                                    cAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTg                                                                                                                                                     <  1:247661/148‑1 (MQ=255) cAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTg                                                                                                                                                     <  7:270424/148‑1 (MQ=255)  aagaagCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGc                                                                                                                                                   >  7:182777/1‑149 (MQ=255)           gTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAAcc                                                                                                                                          >  1:18480/1‑149 (MQ=255)              tCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGa                                                                                                                                        <  2:100390/148‑1 (MQ=255)              tCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGGCGAGCACCACTACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGa                                                                                                                                        <  5:77727/148‑1 (MQ=255)                acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTTACGACGCCACCGAgg                                                                                                                                      <  2:30378/148‑1 (MQ=255)                acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAgg                                                                                                                                      >  5:54214/1‑148 (MQ=255)                acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAgg                                                                                                                                      >  5:48806/1‑148 (MQ=255)                 cccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGaa                                                                                                                                    <  2:99541/149‑1 (MQ=255)                     ccTGTCCACCGGACGCGGCGTTGAACGGCGTGCGGTCGAGCACCAGAACGCCCTCCTCGCCTTCACCCAGCAGGTCATCGGATTGGCCGTCCTTGTACAGGGCGTTGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCg                                                                                                                                <  2:268112/149‑1 (MQ=255)                        gACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTg                                                                                                                             >  1:321465/1‑149 (MQ=255)                          ccaccGGACTCGGCGTAGAACGGCGTGCGGGCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGAATGCCCGGCCTTGTACAGGGCGATGAGCTGGCCCTGGCCCGCGGTGGCGTCGTCACCGAGGAACTCGGTGgc                                                                                                                           >  5:355366/1‑149 (MQ=255)                             ccGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCt                                                                                                                        >  3:192396/1‑149 (MQ=255)                             ccGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCCTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGTTCCTCGGttacgac                                                                                                                        <  3:117682/149‑7 (MQ=255)                              cGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTg                                                                                                                       >  4:66154/1‑149 (MQ=255)                              cGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTAGTACAGGGCGATGATCTTGCCCTGGCCCTAGGTGGCGTCGTAACCGATGAACTCGGTGTCGCTg                                                                                                                       >  4:265154/1‑149 (MQ=255)                                   tCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGAc                                                                                                                  >  2:281837/1‑149 (MQ=255)                                     ggCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGAcc                                                                                                                 <  8:121367/148‑1 (MQ=255)                                     ggCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCt                                                                                                                >  5:101367/1‑149 (MQ=255)                                      gCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCtt                                                                                                               <  2:281827/149‑1 (MQ=255)                                         tAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGAc                                                                                                            <  6:101367/149‑1 (MQ=255)                                           gAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                          <  6:151426/149‑1 (MQ=255)                                            aaCGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAg                                                                                                         >  4:23946/1‑149 (MQ=255)                                               ggCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCt                                                                                                      >  7:240800/1‑149 (MQ=255)                                                  gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                          <  5:256749/142‑1 (MQ=255)                                                  gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                          >  6:256749/1‑142 (MQ=255)                                                  gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCtgtt                                                                                                   <  5:320773/149‑1 (MQ=255)                                                      ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGt                                                                                                                      <  7:10005/126‑1 (MQ=255)                                                      ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGt                                                                                                                      >  8:10005/1‑126 (MQ=255)                                                      ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTaa                                                                                               <  7:24757/149‑1 (MQ=255)                                                         cGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATcc                                                                                            <  6:54214/149‑1 (MQ=255)                                                          gAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATcc                                                                                            <  6:48806/148‑1 (MQ=255)                                                           aGCACCACAACGCCCTGCTCGCCATCATCGAGCTGGTGATCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGAGCAGGCTGTTGTAATCCAt                                                                                          <  3:265154/149‑1 (MQ=255)                                                           aGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCAt                                                                                          <  8:182777/149‑1 (MQ=255)                                                            gCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGTCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATg                                                                                         >  4:215009/1‑149 (MQ=255)                                                                   aaCGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAgg                                                                                  <  4:205025/149‑1 (MQ=255)                                                                          tcctcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCGGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGc                                                                           >  4:91937/1‑149 (MQ=255)                                                                            ctcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCg                                                                          >  4:121992/1‑148 (MQ=255)                                                                             tcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCg                                                                          >  3:123319/1‑147 (MQ=255)                                                                               gCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGc                                                                      >  5:340546/1‑149 (MQ=255)                                                                                 cTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCgg                                                                    <  2:284596/149‑1 (MQ=255)                                                                                     aCCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgcgc                                                                <  1:338105/149‑1 (MQ=255)                                                                                       ccAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgc                                                                  >  3:210540/1‑145 (MQ=255)                                                                                       ccAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgc                                                                  <  4:210540/145‑1 (MQ=255)                                                                                               tCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTg                                                      <  3:23946/149‑1 (MQ=255)                                                                                                 aaCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTggg                                                    >  4:226799/1‑149 (MQ=255)                                                                                                 aaCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTggg                                                    >  5:120551/1‑149 (MQ=255)                                                                                                  aCGGATTGGCCGTGCTTGGACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGGGGCGCTGTCGACCTTGACCAGGCTGTTGTAAGCCATGCCGACGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGc                                                   <  6:355366/149‑1 (MQ=255)                                                                                                  aCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGCCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGc                                                   <  1:281837/149‑1 (MQ=255)                                                                                                    ggATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGt                                                 <  8:240800/149‑1 (MQ=255)                                                                                                       ttGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCa                                              <  3:66154/149‑1 (MQ=255)                                                                                                          gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                           >  4:7957/1‑149 (MQ=255)                                                                                                          gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                           >  6:141306/1‑149 (MQ=255)                                                                                                          gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                           >  5:70789/1‑149 (MQ=255)                                                                                                            cGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCCTTTCa                                         >  3:5113/1‑149 (MQ=255)                                                                                                             gTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCAc                                        <  3:226799/149‑1 (MQ=255)                                                                                                             gTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCAc                                        >  4:311387/1‑149 (MQ=255)                                                                                                                 ttGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTc                                    >  1:10597/1‑149 (MQ=255)                                                                                                                   gTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTc                                    <  6:120551/147‑1 (MQ=255)                                                                                                                      cAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGc                               >  4:75536/1‑149 (MQ=255)                                                                                                                       aGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCa                                         <  6:76833/138‑1 (MQ=255)                                                                                                                       aGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCa                                         >  5:76833/1‑138 (MQ=255)                                                                                                                                 tCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTgg                                                     <  4:300564/116‑1 (MQ=255)                                                                                                                                 tCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTgg                                                     >  3:300564/1‑116 (MQ=255)                                                                                                                                     gCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGAt                  <  4:123319/147‑1 (MQ=255)                                                                                                                                       ccTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGt               >  4:300352/1‑148 (MQ=255)                                                                                                                                        gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                               >  7:127840/6‑115 (MQ=255)                                                                                                                                        gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                               <  4:48100/110‑1 (MQ=255)                                                                                                                                        gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                               >  3:48100/6‑115 (MQ=255)                                                                                                                                        gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                               <  8:127840/110‑1 (MQ=255)                                                                                                                                          ggCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgc           >  3:256236/1‑149 (MQ=255)                                                                                                                                              cTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcac       <  5:141306/149‑1 (MQ=255)                                                                                                                                                   tGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcacgctca  >  2:119557/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCA  >  NC_002947/5082736‑5083030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑