Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,082,884	G→A	7.0%	A461V (GCC→GTC) ‡	alaS ←	alanine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,082,884	0	G	A	7.0%	209.9 / 4.3	72	A461V (GCC→GTC) ‡	alaS	alanine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base A (2/3);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

AAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCAC  >  NC_002947/5082737‑5083031                                                                                                                                                    |                                                                                                                                                    aagaagCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGc                                                                                                                                                    >  7:182777/1‑149 (MQ=255)          gTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAAcc                                                                                                                                           >  1:18480/1‑149 (MQ=255)             tCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGa                                                                                                                                         <  2:100390/148‑1 (MQ=255)             tCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGGCGAGCACCACTACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGa                                                                                                                                         <  5:77727/148‑1 (MQ=255)               acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTTACGACGCCACCGAgg                                                                                                                                       <  2:30378/148‑1 (MQ=255)               acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAgg                                                                                                                                       >  5:48806/1‑148 (MQ=255)               acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAgg                                                                                                                                       >  5:54214/1‑148 (MQ=255)                cccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGaa                                                                                                                                     <  2:99541/149‑1 (MQ=255)                    ccTGTCCACCGGACGCGGCGTTGAACGGCGTGCGGTCGAGCACCAGAACGCCCTCCTCGCCTTCACCCAGCAGGTCATCGGATTGGCCGTCCTTGTACAGGGCGTTGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCg                                                                                                                                 <  2:268112/149‑1 (MQ=255)                       gACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTg                                                                                                                              >  1:321465/1‑149 (MQ=255)                         ccaccGGACTCGGCGTAGAACGGCGTGCGGGCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGAATGCCCGGCCTTGTACAGGGCGATGAGCTGGCCCTGGCCCGCGGTGGCGTCGTCACCGAGGAACTCGGTGgc                                                                                                                            >  5:355366/1‑149 (MQ=255)                            ccGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCt                                                                                                                         >  3:192396/1‑149 (MQ=255)                            ccGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCCTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGTTCCTCGGttacgac                                                                                                                         <  3:117682/149‑7 (MQ=255)                             cGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTg                                                                                                                        >  4:66154/1‑149 (MQ=255)                             cGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTAGTACAGGGCGATGATCTTGCCCTGGCCCTAGGTGGCGTCGTAACCGATGAACTCGGTGTCGCTg                                                                                                                        >  4:265154/1‑149 (MQ=255)                                  tCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGAc                                                                                                                   >  2:281837/1‑149 (MQ=255)                                    ggCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGAcc                                                                                                                  <  8:121367/148‑1 (MQ=255)                                    ggCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCt                                                                                                                 >  5:101367/1‑149 (MQ=255)                                     gCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCtt                                                                                                                <  2:281827/149‑1 (MQ=255)                                        tAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGAc                                                                                                             <  6:101367/149‑1 (MQ=255)                                          gAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                           <  6:151426/149‑1 (MQ=255)                                           aaCGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAg                                                                                                          >  4:23946/1‑149 (MQ=255)                                              ggCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCt                                                                                                       >  7:240800/1‑149 (MQ=255)                                                 gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                           >  6:256749/1‑142 (MQ=255)                                                 gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                           <  5:256749/142‑1 (MQ=255)                                                 gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCtgtt                                                                                                    <  5:320773/149‑1 (MQ=255)                                                     ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGt                                                                                                                       >  8:10005/1‑126 (MQ=255)                                                     ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGt                                                                                                                       <  7:10005/126‑1 (MQ=255)                                                     ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTaa                                                                                                <  7:24757/149‑1 (MQ=255)                                                        cGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATcc                                                                                             <  6:54214/149‑1 (MQ=255)                                                         gAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATcc                                                                                             <  6:48806/148‑1 (MQ=255)                                                          aGCACCACAACGCCCTGCTCGCCATCATCGAGCTGGTGATCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGAGCAGGCTGTTGTAATCCAt                                                                                           <  3:265154/149‑1 (MQ=255)                                                          aGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCAt                                                                                           <  8:182777/149‑1 (MQ=255)                                                           gCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGTCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATg                                                                                          >  4:215009/1‑149 (MQ=255)                                                                  aaCGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAgg                                                                                   <  4:205025/149‑1 (MQ=255)                                                                         tcctcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCGGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGc                                                                            >  4:91937/1‑149 (MQ=255)                                                                           ctcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCg                                                                           >  4:121992/1‑148 (MQ=255)                                                                            tcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCg                                                                           >  3:123319/1‑147 (MQ=255)                                                                              gCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGc                                                                       >  5:340546/1‑149 (MQ=255)                                                                                cTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCgg                                                                     <  2:284596/149‑1 (MQ=255)                                                                                    aCCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgcgc                                                                 <  1:338105/149‑1 (MQ=255)                                                                                      ccAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgc                                                                   <  4:210540/145‑1 (MQ=255)                                                                                      ccAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgc                                                                   >  3:210540/1‑145 (MQ=255)                                                                                              tCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTg                                                       <  3:23946/149‑1 (MQ=255)                                                                                                aaCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTggg                                                     >  5:120551/1‑149 (MQ=255)                                                                                                aaCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTggg                                                     >  4:226799/1‑149 (MQ=255)                                                                                                 aCGGATTGGCCGTGCTTGGACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGGGGCGCTGTCGACCTTGACCAGGCTGTTGTAAGCCATGCCGACGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGc                                                    <  6:355366/149‑1 (MQ=255)                                                                                                 aCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGCCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGc                                                    <  1:281837/149‑1 (MQ=255)                                                                                                   ggATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGt                                                  <  8:240800/149‑1 (MQ=255)                                                                                                      ttGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCa                                               <  3:66154/149‑1 (MQ=255)                                                                                                         gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                            >  5:70789/1‑149 (MQ=255)                                                                                                         gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                            >  4:7957/1‑149 (MQ=255)                                                                                                         gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                            >  6:141306/1‑149 (MQ=255)                                                                                                           cGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCCTTTCa                                          >  3:5113/1‑149 (MQ=255)                                                                                                            gTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCAc                                         <  3:226799/149‑1 (MQ=255)                                                                                                            gTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCAc                                         >  4:311387/1‑149 (MQ=255)                                                                                                                ttGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTc                                     >  1:10597/1‑149 (MQ=255)                                                                                                                  gTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTc                                     <  6:120551/147‑1 (MQ=255)                                                                                                                     cAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGc                                >  4:75536/1‑149 (MQ=255)                                                                                                                      aGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCa                                          <  6:76833/138‑1 (MQ=255)                                                                                                                      aGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCa                                          >  5:76833/1‑138 (MQ=255)                                                                                                                                tCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTgg                                                      <  4:300564/116‑1 (MQ=255)                                                                                                                                tCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTgg                                                      >  3:300564/1‑116 (MQ=255)                                                                                                                                    gCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGAt                   <  4:123319/147‑1 (MQ=255)                                                                                                                                      ccTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGt                >  4:300352/1‑148 (MQ=255)                                                                                                                                       gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                <  4:48100/110‑1 (MQ=255)                                                                                                                                       gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                >  7:127840/6‑115 (MQ=255)                                                                                                                                       gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                >  3:48100/6‑115 (MQ=255)                                                                                                                                       gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                <  8:127840/110‑1 (MQ=255)                                                                                                                                         ggCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgc            >  3:256236/1‑149 (MQ=255)                                                                                                                                             cTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcac        <  5:141306/149‑1 (MQ=255)                                                                                                                                                  tGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcacgctca   >  2:119557/1‑149 (MQ=255)                                                                                                                                                    gCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcacgctcac  >  6:20992/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AAGAAGCCGGTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCAC  >  NC_002947/5082737‑5083031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑