Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,082,890	T→C	6.9%	Y459C (TAC→TGC) ‡	alaS ←	alanine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,082,890	0	T	C	6.9%	209.1 / 5.1	72	Y459C (TAC→TGC) ‡	alaS	alanine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (35/32);  new base C (2/3);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

GTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCACGAGCGAT  >  NC_002947/5082746‑5083038                                                                                                                                                 |                                                                                                                                                     gTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAAcc                                                                                                                                                  >  1:18480/1‑149 (MQ=255)    tCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGa                                                                                                                                                <  2:100390/148‑1 (MQ=255)    tCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGGCGAGCACCACTACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGa                                                                                                                                                <  5:77727/148‑1 (MQ=255)      acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTTACGACGCCACCGAgg                                                                                                                                              <  2:30378/148‑1 (MQ=255)      acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAgg                                                                                                                                              >  5:48806/1‑148 (MQ=255)      acccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAgg                                                                                                                                              >  5:54214/1‑148 (MQ=255)       cccaccTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGaa                                                                                                                                            <  2:99541/149‑1 (MQ=255)           ccTGTCCACCGGACGCGGCGTTGAACGGCGTGCGGTCGAGCACCAGAACGCCCTCCTCGCCTTCACCCAGCAGGTCATCGGATTGGCCGTCCTTGTACAGGGCGTTGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCg                                                                                                                                        <  2:268112/149‑1 (MQ=255)              gACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTg                                                                                                                                     >  1:321465/1‑149 (MQ=255)                ccaccGGACTCGGCGTAGAACGGCGTGCGGGCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGAATGCCCGGCCTTGTACAGGGCGATGAGCTGGCCCTGGCCCGCGGTGGCGTCGTCACCGAGGAACTCGGTGgc                                                                                                                                   >  5:355366/1‑149 (MQ=255)                   ccGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCt                                                                                                                                >  3:192396/1‑149 (MQ=255)                   ccGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCCTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGTTCCTCGGttacgac                                                                                                                                <  3:117682/149‑7 (MQ=255)                    cGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTg                                                                                                                               >  4:66154/1‑149 (MQ=255)                    cGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTAGTACAGGGCGATGATCTTGCCCTGGCCCTAGGTGGCGTCGTAACCGATGAACTCGGTGTCGCTg                                                                                                                               >  4:265154/1‑149 (MQ=255)                         tCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGAc                                                                                                                          >  2:281837/1‑149 (MQ=255)                           ggCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGAcc                                                                                                                         <  8:121367/148‑1 (MQ=255)                           ggCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCt                                                                                                                        >  5:101367/1‑149 (MQ=255)                            gCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCtt                                                                                                                       <  2:281827/149‑1 (MQ=255)                               tAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGAc                                                                                                                    <  6:101367/149‑1 (MQ=255)                                 gAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                                  <  6:151426/149‑1 (MQ=255)                                  aaCGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAg                                                                                                                 >  4:23946/1‑149 (MQ=255)                                     ggCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCt                                                                                                              >  7:240800/1‑149 (MQ=255)                                        gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                                  <  5:256749/142‑1 (MQ=255)                                        gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCa                                                                                                                  >  6:256749/1‑142 (MQ=255)                                        gTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCtgtt                                                                                                           <  5:320773/149‑1 (MQ=255)                                            ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGt                                                                                                                              >  8:10005/1‑126 (MQ=255)                                            ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGt                                                                                                                              <  7:10005/126‑1 (MQ=255)                                            ggTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTaa                                                                                                       <  7:24757/149‑1 (MQ=255)                                               cGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATcc                                                                                                    <  6:54214/149‑1 (MQ=255)                                                gAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATcc                                                                                                    <  6:48806/148‑1 (MQ=255)                                                 aGCACCACAACGCCCTGCTCGCCATCATCGAGCTGGTGATCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGAGCAGGCTGTTGTAATCCAt                                                                                                  <  3:265154/149‑1 (MQ=255)                                                 aGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCAt                                                                                                  <  8:182777/149‑1 (MQ=255)                                                  gCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGTCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATg                                                                                                 >  4:215009/1‑149 (MQ=255)                                                         aaCGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAgg                                                                                          <  4:205025/149‑1 (MQ=255)                                                                tcctcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCGGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGc                                                                                   >  4:91937/1‑149 (MQ=255)                                                                  ctcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCg                                                                                  >  4:121992/1‑148 (MQ=255)                                                                   tcGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCg                                                                                  >  3:123319/1‑147 (MQ=255)                                                                     gCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGc                                                                              >  5:340546/1‑149 (MQ=255)                                                                       cTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCgg                                                                            <  2:284596/149‑1 (MQ=255)                                                                           aCCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgcgc                                                                        <  1:338105/149‑1 (MQ=255)                                                                             ccAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgc                                                                          <  4:210540/145‑1 (MQ=255)                                                                             ccAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGgc                                                                          >  3:210540/1‑145 (MQ=255)                                                                                     tCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTg                                                              <  3:23946/149‑1 (MQ=255)                                                                                       aaCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTggg                                                            >  5:120551/1‑149 (MQ=255)                                                                                       aaCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTggg                                                            >  4:226799/1‑149 (MQ=255)                                                                                        aCGGATTGGCCGTGCTTGGACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGGGGCGCTGTCGACCTTGACCAGGCTGTTGTAAGCCATGCCGACGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGc                                                           <  6:355366/149‑1 (MQ=255)                                                                                        aCGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGCCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGc                                                           <  1:281837/149‑1 (MQ=255)                                                                                          ggATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGt                                                         <  8:240800/149‑1 (MQ=255)                                                                                             ttGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCa                                                      <  3:66154/149‑1 (MQ=255)                                                                                                gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                                   >  4:7957/1‑149 (MQ=255)                                                                                                gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                                   >  5:70789/1‑149 (MQ=255)                                                                                                gCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCAttt                                                   >  6:141306/1‑149 (MQ=255)                                                                                                  cGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCCTTTCa                                                 >  3:5113/1‑149 (MQ=255)                                                                                                   gTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCAc                                                <  3:226799/149‑1 (MQ=255)                                                                                                   gTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCAc                                                >  4:311387/1‑149 (MQ=255)                                                                                                       ttGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTc                                            >  1:10597/1‑149 (MQ=255)                                                                                                         gTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTc                                            <  6:120551/147‑1 (MQ=255)                                                                                                            cAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGc                                       >  4:75536/1‑149 (MQ=255)                                                                                                             aGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCa                                                 >  5:76833/1‑138 (MQ=255)                                                                                                             aGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCa                                                 <  6:76833/138‑1 (MQ=255)                                                                                                                       tCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTgg                                                             <  4:300564/116‑1 (MQ=255)                                                                                                                       tCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTgg                                                             >  3:300564/1‑116 (MQ=255)                                                                                                                           gCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGAt                          <  4:123319/147‑1 (MQ=255)                                                                                                                             ccTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGt                       >  4:300352/1‑148 (MQ=255)                                                                                                                              gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                       >  3:48100/6‑115 (MQ=255)                                                                                                                              gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                       >  7:127840/6‑115 (MQ=255)                                                                                                                              gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                       <  4:48100/110‑1 (MQ=255)                                                                                                                              gagttccTCGGTTACGACGCCACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTcc                                                       <  8:127840/110‑1 (MQ=255)                                                                                                                                ggCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgc                   >  3:256236/1‑149 (MQ=255)                                                                                                                                    cTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcac               <  5:141306/149‑1 (MQ=255)                                                                                                                                         tGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcacgctca          >  2:119557/1‑149 (MQ=255)                                                                                                                                           gCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAgctcacgctcac         >  6:20992/1‑148 (MQ=255)                                                                                                                                              gcgtAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGTTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCACGAGc     <  2:321465/148‑1 (MQ=255)                                                                                                                                                gtAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCACGAGCg    <  3:300352/148‑1 (MQ=255)                                                                                                                                                 tAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCACGAGCGAt  <  5:20992/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GTATCACCCACCTGACCACCGGACTCGGCGTAGAACGGCGTGCGGTCGAGCACCACAACGCCCTCCTCGCCTTCACCCAGCTGGTCAACGGATTGGCCGTCCTTGTACAGGGCGATGATCTTGCCCTGGCCCTCGGTGGCGTCGTAACCGAGGAACTCGGTGGCGCTGTCGACCTTGACCAGGCTGTTGTAATCCATGCCGAAGGCGCTGGCGGAGCGGGCGCGCTCACGCTGGGCGTCCATTTCACGCTCGAAGCCGGCTTCGTCGATGGTCAGCTCACGCTCACGAGCGAT  >  NC_002947/5082746‑5083038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑