Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,087,625 A→G 6.1% D395D (GAT→GAC)  astD ← N‑succinylglutamate 5‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,087,6250AG 6.1% 192.5 / 2.5 65D395D (GAT→GAC) astDN‑succinylglutamate 5‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (31/30);  new base G (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

ACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCGGCTGAGTCATTTC  >  NC_002947/5087478‑5087768
                                                                                                                                                   |                                                                                                                                               
aCCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCa                                                                                                                                                 >  2:332284/1‑148 (MQ=255)
  cGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGGAGCCAGAAGTACTGGTAGCGGGCTCTCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCg                                                                                                                                              <  5:194607/149‑1 (MQ=255)
     aCGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATg                                                                                                                                           <  7:168404/149‑1 (MQ=255)
                  ggCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGt                                                                                                                              >  6:154058/1‑149 (MQ=255)
                        cccTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGt                                                                                                                        >  4:333485/1‑149 (MQ=255)
                          cTGTTAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTAGAGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAg                                                                                                                      >  1:317240/1‑149 (MQ=255)
                          cTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAg                                                                                                                      >  7:180372/1‑149 (MQ=255)
                             tCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGc                                                                                                                     <  5:154058/147‑1 (MQ=255)
                               aGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGa                                                                                                                  <  8:122750/148‑1 (MQ=255)
                                   gtttgttCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCAcc                                                                                                             >  5:221114/1‑149 (MQ=255)
                                    tttgttCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCt                                                                                                            >  6:36913/1‑149 (MQ=255)
                                    tttgttCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCt                                                                                                            <  2:109849/149‑1 (MQ=255)
                                        ttCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCaa                                                                                                        >  8:101275/1‑149 (MQ=255)
                                        ttCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCaa                                                                                                        >  7:241923/1‑149 (MQ=255)
                                          ccAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCaa                                                                                                        >  2:129110/1‑147 (MQ=255)
                                             gctGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCg                                                                                                   <  1:15815/147‑1 (MQ=255)
                                                gACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGc                                                                                                >  4:245331/1‑149 (MQ=255)
                                                        cTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAAc                                                                                        >  8:25185/1‑149 (MQ=255)
                                                        cTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAAc                                                                                        <  8:180372/149‑1 (MQ=255)
                                                         tGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAAct                                                                                       >  2:150288/1‑149 (MQ=255)
                                                           cgcgACTGCGCAGCCAGAAGTACTGGTAGCGGGCGCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAActct                                                                                     <  4:258596/149‑1 (MQ=255)
                                                            gcgACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCtt                                                                                    <  4:283426/149‑1 (MQ=255)
                                                             cgACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTc                                                                                   <  8:241923/149‑1 (MQ=255)
                                                             cgACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTc                                                                                   <  3:245331/149‑1 (MQ=255)
                                                             cgACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTc                                                                                   <  1:332284/149‑1 (MQ=255)
                                                               aCTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGt                                                                                 <  2:227569/149‑1 (MQ=255)
                                                                  gcgcAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCg                                                                              >  5:89639/1‑149 (MQ=255)
                                                                      aGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCg                                                                          <  6:89639/149‑1 (MQ=255)
                                                                        ccAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCg                                                                                                   <  2:309153/122‑1 (MQ=255)
                                                                         cAGAAGTACTGGTAGCGTGCTCGCGAATCAGTCAATAAGCCGGCGGCCAGGCCATACTGTGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGTCGAAAAACTCTTCGTCCGGGCGGTc                                                                       <  6:299824/149‑1 (MQ=255)
                                                                          aGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCGTCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGt                                                                        >  3:305701/1‑147 (MQ=255)
                                                                          aGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCGTCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGt                                                                        >  3:59596/1‑147 (MQ=255)
                                                                           gAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAg                                                                     >  5:170212/1‑149 (MQ=255)
                                                                                aCTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCAc                                                                 <  7:101275/148‑1 (MQ=255)
                                                                                  tGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAActct                                                                                     <  2:188697/126‑1 (MQ=255)
                                                                                  tGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAActct                                                                                     >  1:188697/1‑126 (MQ=255)
                                                                                       gCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGa                                                         <  3:333485/149‑1 (MQ=255)
                                                                                           gCTCCCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATc                                                     <  4:220307/149‑1 (MQ=255)
                                                                                               gcgAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTc                                                                                                                             >  5:142654/1‑73 (MQ=255)
                                                                                               gcgAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTc                                                                                                                             <  6:142654/73‑1 (MQ=255)
                                                                                               gcgAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGAc                                                  >  3:157218/1‑148 (MQ=255)
                                                                                                 gAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCGTCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAAt                                               <  4:59596/149‑1 (MQ=255)
                                                                                                 gAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCGTCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAAt                                               <  4:305701/149‑1 (MQ=255)
                                                                                                     cAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGt                                                           >  8:124113/1‑133 (MQ=255)
                                                                                                     cAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGt                                                           <  7:124113/133‑1 (MQ=255)
                                                                                                      aGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCTGTGACATCGACAATGCCCg                                          >  4:38737/1‑149 (MQ=255)
                                                                                                          aaTAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGt                                      >  2:175082/1‑149 (MQ=255)
                                                                                                               gCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCCCGGCAGTGACATCGGCAATGCCCGGGGTccg                                   >  4:300861/1‑145 (MQ=255)
                                                                                                                  ggcggcCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTg                              >  1:170657/1‑149 (MQ=255)
                                                                                                                   gcggcCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTgc                             <  6:170212/149‑1 (MQ=255)
                                                                                                                   gcggcCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTgc                             <  1:175082/149‑1 (MQ=255)
                                                                                                                       ccaggccaTACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCTCGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTgcagca                         >  4:22861/1‑149 (MQ=255)
                                                                                                                       ccaggccaTACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTTAGCAGTgcagca                         >  7:240301/1‑149 (MQ=255)
                                                                                                                        caggccaTACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCAt                        <  1:150288/149‑1 (MQ=255)
                                                                                                                                  tGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGGAGTGCAGCATCGGCCTGCgg              >  1:153446/1‑149 (MQ=255)
                                                                                                                                  tGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCgg              >  4:225849/1‑149 (MQ=255)
                                                                                                                                      tgtTATTGGCTTCATCGATGGCGGCATCGAAGTCGTCGTAGCTGATCACCTGCAACAGCGGTCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACTGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCGGCTGa          <  2:317240/149‑1 (MQ=255)
                                                                                                                                      tgtTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTTAGCAGTGCAGCATCGGCCTGCGGCTGa          <  8:240301/149‑1 (MQ=255)
                                                                                                                                              gCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAg                                                            >  2:224533/1‑91 (MQ=255)
                                                                                                                                              gCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGa                                                   <  7:31030/100‑1 (MQ=255)
                                                                                                                                              gCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGa                                                   >  8:31030/1‑100 (MQ=255)
                                                                                                                                              gCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCGGCTGAGTCATTTc  <  6:221114/149‑1 (MQ=255)
                                                                                                                                              gCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCGGCTGAGTCATTTc  >  4:41194/1‑149 (MQ=255)
                                                                                                                                              gCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCGGCTGAGTCATTTc  <  3:221404/149‑1 (MQ=255)
                                                                                                                                              gCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGAAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACAACGACAAAGCCCTGGGTCAGCAGTGCAGCATCGGCCTGCCGCTGAGTCAttt   >  4:221404/1‑148 (MQ=255)
                                                                                                                                                ttCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCGGCTGAGTCATTTc  >  3:332119/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ACCGAACGGCGCGCTGCTGGCAGCCCCTGTCAGCTGTTTGTTCCAGTTGACGATGCCTGCGCGACTGCGCAGCCAGAAGTACTGGTAGCGGGCTCGCGAATCAGACAATAAGCCGGCGGCCAGGCCATACTGGGTGTTATTGGCTTCATCGATGGCGGCATCGAAGTCGGCGTAGCGGATCACCTGCAACAGCGGGCCGAAAAACTCTTCGTCCGGGCGGTCAGCCACGGCAGTGACATCGACAATGCCCGGGGTCAGCAGTGCAGCATCGGCCTGCGGCTGAGTCATTTC  >  NC_002947/5087478‑5087768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑