Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,100,039 A→T 8.3% intergenic (+125/+28) PP_4488 → / ← phhR hypothetical protein/sigma‑54 dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,100,0390AT 8.3% 165.9 / 5.1 60intergenic (+125/+28)PP_4488/phhRhypothetical protein/sigma‑54 dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (29/26);  new base T (2/3);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

TGCTCCAACGGCTGATACCTTGGAATCGGGGGCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAG  >  NC_002947/5099900‑5100186
                                                                                                                                           |                                                                                                                                                   
ggcgccACCGGCTGATACGTTGTAATCGGGGGCCGTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACACATATTGCGCTCAGCCAGAGCACAGCTTTTTACCTGTGGTTGCGGCCTTGTGTCGCGAAAGGACTGCGTTGCAGTcct                                                                                                                                            <  2:347264/145‑2 (MQ=255)
  ctCCAACGGCTTATACCTTGGAATCGGGGGCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCGTTGCAGTcct                                                                                                                                            <  3:205048/147‑2 (MQ=255)
  caccAACGGCTGATACCTTGCATCCGGGGGCCCGGCGGCCCCCTTTCGCGACCCACGCCCGCTCCAACATAAAATGCGCTCACCCATAGCACCGCTAATTACCTGTGGGAGCGGCCTTTTGTCGCGAAAGGACTGCAACGCAGTCCCAg                                                                                                                                          <  4:167911/147‑1 (MQ=255)
    ccAACGGCTGATACCTTGGAATCGGGGGCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTAAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGt                                                                                                                                        >  4:230962/1‑149 (MQ=255)
                  cTTGGAATCGGGGGCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTACCACAGATAATGCGCTAAGCCAGAGCCCAGCGTATTACATGTGGGAGCGGCCTTGTGTGGCGAAATGACTGCAACGCAGTACCAGGTTCTTTTACcaaca                                                                                                                           >  3:110716/1‑148 (MQ=255)
                     ggAATCGGGGGCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCaacaac                                                                                                                         <  7:342709/147‑1 (MQ=255)
                        aTCGGGGGCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATc                                                                                                                    >  1:15624/1‑149 (MQ=255)
                           gggggCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATccgc                                                                                                                 >  8:90299/1‑149 (MQ=255)
                              ggCCCTGCGGCCCCCTATCGCGACACAAGGCCGCTCCAACATATAATGCGCTCACCCAGACCACCGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGa                                                                                                              <  3:13578/149‑1 (MQ=39)
                                 ccTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACtt                                                                                                           <  1:50406/149‑1 (MQ=255)
                                        gCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGc                                                                                                    <  1:53957/149‑1 (MQ=255)
                                          cccTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGa                                                                                                  <  2:15624/149‑1 (MQ=255)
                                          cccTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACAGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGa                                                                                                  <  5:6314/149‑1 (MQ=255)
                                                  cgACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACt                                                                                                            >  6:33234/1‑131 (MQ=255)
                                                  cgACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACt                                                                                                            <  5:33234/131‑1 (MQ=255)
                                                   gACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCt                                                                                         <  4:283848/149‑1 (MQ=255)
                                                      acaAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGc                                                                                                    >  7:185451/1‑135 (MQ=255)
                                                      acaAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGc                                                                                                    <  8:185451/135‑1 (MQ=255)
                                                      acaAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGAGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCaa                                                                                      <  1:302899/149‑1 (MQ=255)
                                                       caAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAAc                                                                                     <  7:90299/149‑1 (MQ=255)
                                                        aaGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACt                                                                                    >  7:198347/1‑149 (MQ=255)
                                                        aaGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACt                                                                                    >  7:303052/1‑149 (MQ=255)
                                                        aaGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACt                                                                                    >  3:340870/1‑149 (MQ=255)
                                                        aaGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACt                                                                                    >  5:227291/1‑149 (MQ=255)
                                                           gCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACttgt                                                                                 >  6:321929/1‑149 (MQ=255)
                                                            ccGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGAGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCACACCttgtt                                                                                >  4:321841/1‑149 (MQ=255)
                                                                tCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGc                                                                             >  6:61172/1‑148 (MQ=255)
                                                                  ccACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCaa                                                                           <  8:198347/148‑1 (MQ=255)
                                                                  ccACACATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGACCTTCTGTCGCGAAACGACTACAACGCAGTCACATGTTCTTTGACAAACAAATAATCAGCAGACTTCATAAGCTAGTAATCCCTCAACTTCTAGGCAAt                                                                          >  4:256814/1‑149 (MQ=255)
                                                                      aGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTc                                                                      <  5:61172/149‑1 (MQ=255)
                                                                          aaTGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCgtgt                                                                  >  8:245585/1‑149 (MQ=255)
                                                                               gcTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGaa                                                             <  3:230962/149‑1 (MQ=255)
                                                                                 tCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATcc                                                                                                                   <  4:306047/93‑1 (MQ=255)
                                                                                 tCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATcc                                                                                                                   >  3:306047/1‑93 (MQ=255)
                                                                                     ccAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACAcc                                                        >  2:230048/1‑148 (MQ=255)
                                                                                      cAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACAcc                                                        >  2:11927/1‑147 (MQ=255)
                                                                                       agagCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTgg                                                                              <  8:278164/124‑1 (MQ=255)
                                                                                       agagCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTgg                                                                              >  7:278164/1‑124 (MQ=255)
                                                                                        gagCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAac                                                           <  4:27381/142‑1 (MQ=255)
                                                                                        gagCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAac                                                           >  3:27381/1‑142 (MQ=255)
                                                                                             caGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCtttt                                               <  8:303052/149‑1 (MQ=255)
                                                                                               gCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAg                                                                                                 >  8:256565/1‑97 (MQ=255)
                                                                                               gCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAg                                                                                                 <  7:256565/97‑1 (MQ=255)
                                                                                                cGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGcc                                            <  1:11927/149‑1 (MQ=255)
                                                                                                      aCCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAgg                                       <  4:340870/148‑1 (MQ=255)
                                                                                                             ggAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGact                                >  7:347349/1‑148 (MQ=255)
                                                                                                                      ttGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAAc                      >  1:151035/1‑149 (MQ=255)
                                                                                                                           tCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTcccc                 >  2:348931/1‑149 (MQ=255)
                                                                                                                           tCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTcccc                 <  6:227291/149‑1 (MQ=255)
                                                                                                                             gcgAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTccccc                <  1:51074/148‑1 (MQ=255)
                                                                                                                              cgAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCAt              >  4:216828/1‑149 (MQ=255)
                                                                                                                                ctgggACTGCGTTGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCACAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTccc                  <  2:183346/140‑1 (MQ=255)
                                                                                                                                ctgggACTGCGTTGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCACAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTccc                  >  1:183346/4‑143 (MQ=255)
                                                                                                                                 tgggACTGCGTTGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACGACACCATCa            >  8:326699/3‑148 (MQ=255)
                                                                                                                                      cTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACttt      >  6:154492/1‑149 (MQ=255)
                                                                                                                                       tGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCTATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTgg                           <  6:79606/127‑1 (MQ=255)
                                                                                                                                       tGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTgg                           >  5:79606/1‑127 (MQ=255)
                                                                                                                                       tGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTc     >  7:240934/1‑149 (MQ=255)
                                                                                                                                       tGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGAGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACttt      >  4:10753/1‑148 (MQ=255)
                                                                                                                                         cAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTc     <  2:151035/147‑1 (MQ=255)
                                                                                                                                           aCGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAg  >  7:69991/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
TGCTCCAACGGCTGATACCTTGGAATCGGGGGCCCTGCGGGCCCCTATCGCGACACAAGGCCGCTCCCACAGATAATGCGCTCAGCCAGAGCACAGCGTATTACCTGTGGGAGCGGCCTTGTGTCGCGAAAGGACTGCAACGCAGTCCCAGGTTCTTTGACCAACAACTAATCCGCCGACTTGTTAAGCGAGTAATCCCTCAACTTGTTGGCAATTGTCGTGTGCGAAACACCCAGTCTTTTGCCCAAGGCCCGACTACTGGGAAACTCCCCCATCAAACTTTCCAG  >  NC_002947/5099900‑5100186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑