Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,105,668	G→A	8.3%	P815L (CCG→CTG)	ydiJ ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,105,668	0	G	A	8.3%	206.8 / 6.4	72	P815L (CCG→CTG)	ydiJ	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (38/28);  new base A (2/4);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

ACACCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCCGG  >  NC_002947/5105522‑5105813                                                                                                                                                   |                                                                                                                                                  acacCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGgc                                                                                                                                                 <  7:340553/149‑1 (MQ=255)   acCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGgcgc                                                                                                                                               >  3:243100/1‑149 (MQ=255)   acCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGACGTACAgcgc                                                                                                                                               >  3:161183/1‑149 (MQ=255)     cAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGgcgc                                                                                                                                               >  2:284079/1‑147 (MQ=255)      aGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCCGTACAGCGCCaa                                                                                                                                            <  3:57579/149‑2 (MQ=255)       gCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCCGTACAGCGCCaac                                                                                                                                           <  4:161183/149‑3 (MQ=255)       gCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCCGTACAGCGCCaac                                                                                                                                           <  8:261727/149‑3 (MQ=255)           cATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGCTGGCGCCGTACAGCGCCaacggca                                                                                                                                       <  7:332303/149‑7 (MQ=255)                ccGGGTCCTGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCgg                                                                                                                                  >  6:52991/1‑149 (MQ=255)                ccGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCgg                                                                                                                                  >  7:1354/1‑149 (MQ=255)                ccGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACCCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCgg                                                                                                                                  >  1:267832/1‑149 (MQ=255)                 cGGGTCCCGCCCCACCTGCGCCACACCGCAAGCGGCCAGGGCCTTGAGAGGCGTGGGGTTGCATAAGGCTGCATTGGCAAAGCGGCCAAGGAAACCCTGTACGTCCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGt                                                                                                                                 <  1:76646/149‑1 (MQ=255)                     tCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGcc                                                                                                                             >  4:127776/1‑149 (MQ=255)                       cAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGccc                                                                                                                            <  5:188554/148‑1 (MQ=255)                          ccccACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTg                                                                                                                                >  3:9172/1‑141 (MQ=255)                          ccccACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTg                                                                                                                                <  4:9172/141‑1 (MQ=255)                          ccccACCAGCGGCACAACGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGACCAGGc                                                                                                                        >  2:322997/1‑149 (MQ=255)                            ccaccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCgg                                                                                                                      >  6:124245/1‑149 (MQ=255)                              accaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTg                                                                                                                    >  2:346772/1‑149 (MQ=255)                                     gCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAgg                                                                                                             <  5:124245/149‑1 (MQ=255)                                      cacaCCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGt                                                                                                            <  8:255772/149‑1 (MQ=255)                                         acCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGa                                                                                                         <  3:229707/149‑1 (MQ=255)                                           cGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGAt                                                                                                        >  8:220180/1‑148 (MQ=255)                                           cGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGAt                                                                                                        >  6:246406/1‑148 (MQ=255)                                                   ggctgggCCTTGAGCTGCGTGGAGTTGCGGATCTCTTCGTTGGCAAACGCGCCAAGGAAAGGCTGTACTTGCAACGGCTTGCCGTTGGCGATGTACGGCGCCAGTAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCTATGAAGGCCGa                                                                                               <  8:273393/145‑1 (MQ=255)                                                          ccTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGAc                                                                                              >  6:87101/1‑143 (MQ=255)                                                          ccTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGAc                                                                                              <  5:87101/143‑1 (MQ=255)                                                            ttGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAgcgg                                                                                      >  5:359065/1‑149 (MQ=255)                                                              gAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAgcggc                                                                                     <  5:219616/148‑1 (MQ=255)                                                               aGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGt                                                                                   >  2:206215/1‑149 (MQ=255)                                                                          gTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGc                                                                        >  6:75090/1‑149 (MQ=255)                                                                             gCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCggg                                                                     >  8:112146/1‑149 (MQ=255)                                                                                  tCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAg                                                                <  2:229168/149‑1 (MQ=255)                                                                                  tCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAg                                                                <  5:246406/149‑1 (MQ=255)                                                                                  tCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAg                                                                >  1:184840/1‑149 (MQ=255)                                                                                    gCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGc                                                              <  3:127776/149‑1 (MQ=255)                                                                                     cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                      <  3:80198/140‑1 (MQ=255)                                                                                     cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                      >  4:80198/1‑140 (MQ=255)                                                                                     cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCAGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCg                                                             >  4:232193/1‑149 (MQ=255)                                                                                        ccTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTcc                                                          <  2:355138/149‑1 (MQ=255)                                                                                         cTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAg                                                                                                    <  7:324226/106‑1 (MQ=255)                                                                                         cTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAg                                                                                                    >  8:324226/1‑106 (MQ=255)                                                                                         cTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCt                                                         <  3:232193/149‑1 (MQ=255)                                                                                            ggCAAACGCGCCAAGGAATGCCTGTACGTGCAACGGCTTGGCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGc                                                       <  6:359065/148‑1 (MQ=255)                                                                                            ggCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCa                                                      >  3:261282/1‑149 (MQ=255)                                                                                             gCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCggg                                                                     >  5:94946/1‑133 (MQ=255)                                                                                             gCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCggg                                                                     <  6:94946/133‑1 (MQ=255)                                                                                              cAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCAcc                                                    <  8:1354/149‑1 (MQ=255)                                                                                               aaaCGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCa                                                   >  4:361044/1‑149 (MQ=255)                                                                                                 aCGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGc                                                 <  5:52991/149‑1 (MQ=255)                                                                                                      ccAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGAt                                            >  3:15510/1‑149 (MQ=255)                                                                                                       cAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCt                                                         >  5:82271/1‑135 (MQ=255)                                                                                                       cAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCt                                                         <  6:82271/135‑1 (MQ=255)                                                                                                              aCCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCt                                    >  7:120399/1‑149 (MQ=255)                                                                                                                ccTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTcg                                  >  5:72517/1‑149 (MQ=255)                                                                                                                 cTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTcgc                                 >  1:24778/1‑149 (MQ=255)                                                                                                                 cTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTcgc                                 <  1:284079/149‑1 (MQ=255)                                                                                                                     aCGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTg                             >  8:157406/1‑149 (MQ=255)                                                                                                                         gCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAg                                                                >  3:290172/1‑110 (MQ=255)                                                                                                                         gCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAg                                                                <  4:290172/110‑1 (MQ=255)                                                                                                                           aaCGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCgg                       >  4:146002/1‑149 (MQ=255)                                                                                                                            aCGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCggg                      <  6:238860/149‑1 (MQ=255)                                                                                                                             cGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCgggg                     >  8:3021/1‑149 (MQ=255)                                                                                                                                 ttGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGt                    >  7:153651/1‑146 (MQ=255)                                                                                                                                 ttGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGt                    <  8:153651/146‑1 (MQ=255)                                                                                                                                    ttcctGGCGCCGTACAGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTc              >  3:245673/5‑149 (MQ=255)                                                                                                                                          gcgcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAg        <  7:157406/149‑1 (MQ=255)                                                                                                                                           cgcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAgg       <  1:346772/149‑1 (MQ=255)                                                                                                                                            gcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAggg      >  7:204061/1‑149 (MQ=255)                                                                                                                                              tGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGcc    >  4:336965/1‑149 (MQ=255)                                                                                                                                              tGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGcc    >  5:127525/1‑149 (MQ=255)                                                                                                                                                tACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCCgg  >  5:184634/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ACACCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCCGG  >  NC_002947/5105522‑5105813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑