Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,156,328 A→G 6.7% S263G (AGC→GGC)  PP_4539 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,156,3280AG 6.7% 175.5 / 2.6 61S263G (AGC→GGC) PP_4539LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (27/29);  new base G (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

CGCCGAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGACCGC  >  NC_002947/5156180‑5156474
                                                                                                                                                    |                                                                                                                                                  
cgcCGAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCa                                                                                                                                                    <  4:288174/149‑1 (MQ=255)
   cGAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCGGGGCTTTGCCTGGCTGCCCCGTCACATGAGCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAgcc                                                                                                                                                 >  4:246205/1‑149 (MQ=255)
    gAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAgccg                                                                                                                                                >  3:140259/1‑149 (MQ=255)
     agcagcGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAgccgc                                                                                                                                               >  7:351435/1‑149 (MQ=255)
         gcgcTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGccacc                                                                                                                                           <  7:139113/149‑1 (MQ=255)
           gcTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccct                                                                                                                                         <  8:255752/149‑2 (MQ=255)
               gACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccctgat                                                                                                                                      <  4:158061/148‑5 (MQ=255)
               gACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccctgat                                                                                                                                      >  3:158061/1‑144 (MQ=255)
                            cTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGGGGCAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                        <  8:188513/149‑1 (MQ=255)
                               ggCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAg                                                                                                                     <  6:24130/149‑1 (MQ=255)
                                gCACCGCCGCCACCTTCGTCAGCAGCGGCCTTGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGc                                                                                                                    <  6:197958/149‑1 (MQ=255)
                                  aCCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGccacc                                                                                                                                           >  8:301492/1‑124 (MQ=255)
                                  aCCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGccacc                                                                                                                                           <  7:301492/124‑1 (MQ=255)
                                              ttCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCt                                                                                                      >  2:273966/1‑149 (MQ=255)
                                                      cagcGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCgg                                                                                              >  1:82518/1‑149 (MQ=255)
                                                        gcGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGcc                                                                                            <  3:339176/149‑1 (MQ=255)
                                                         cGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCa                                                                                           >  6:281798/1‑149 (MQ=255)
                                                                  cTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGAtt                                                                                   >  2:42386/1‑148 (MQ=255)
                                                                  cTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATtc                                                                                  <  8:351435/149‑1 (MQ=255)
                                                                  cTTTGCCTGGCTGCCCCGCCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGGCGCTGGATCAGGGTGGCAGCCGCCACCGACTGTTCTCCCTTTATTCGAGGAAAGAGAAGACCCTGGGCCCGGCCACGAAGATtc                                                                                  >  2:56865/1‑149 (MQ=255)
                                                                  cTTTGCCTGGCGGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGCAGACCCTGGGCCCGGCCACGCAGATtc                                                                                  <  2:82518/149‑1 (MQ=255)
                                                                           gCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGAc                                                                          <  4:140259/148‑1 (MQ=255)
                                                                             tGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCttt                                                                                                                            >  7:289822/1‑96 (MQ=255)
                                                                             tGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCttt                                                                                                                            <  8:289822/96‑1 (MQ=255)
                                                                              gCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACctgc                                                                      >  7:157376/1‑149 (MQ=255)
                                                                               ccccGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACctgc                                                                      <  4:241170/148‑1 (MQ=255)
                                                                                cccGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCCCGCAGATTCTCATCGACctgctg                                                                    >  8:21198/1‑149 (MQ=255)
                                                                                 ccGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCtgctgc                                                                   >  8:19451/1‑149 (MQ=255)
                                                                                  cGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCTCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTgcg                                                                  >  2:260329/1‑149 (MQ=255)
                                                                                      acaTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCCGCCGCCACCCACTGTTCTACCTGTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAAt                                                              >  4:228224/1‑149 (MQ=255)
                                                                                       caTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGa                                                                                     >  1:131128/1‑125 (MQ=255)
                                                                                       caTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGa                                                                                     <  2:131128/125‑1 (MQ=255)
                                                                                       caTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAAtt                                                             >  2:369376/1‑149 (MQ=255)
                                                                                        aTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAAttt                                                            >  6:43307/1‑149 (MQ=255)
                                                                                        aTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGACAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                        >  7:188308/1‑89 (MQ=255)
                                                                                        aTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGACAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                        <  8:188308/89‑1 (MQ=255)
                                                                                        aTGATCGAGCGCGAGCTGCCCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATACTCAACGACCTCCTGCGAAAtt                                                             >  6:243655/1‑148 (MQ=255)
                                                                                         tGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                           <  1:369376/149‑1 (MQ=255)
                                                                                                   cgAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACCCCgcgc                                                 >  8:158862/1‑149 (MQ=255)
                                                                                                   cgAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCgcgc                                                 <  3:306086/149‑1 (MQ=255)
                                                                                                     aGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCa                                               <  8:178677/149‑1 (MQ=255)
                                                                                                      gCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCAc                                              <  6:53186/149‑1 (MQ=255)
                                                                                                       cTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACt                                             <  3:246205/149‑1 (MQ=255)
                                                                                                            cgAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                           <  3:219297/130‑1 (MQ=255)
                                                                                                            cgAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                           >  4:219297/1‑130 (MQ=255)
                                                                                                             gAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGt                                       >  3:309204/1‑149 (MQ=255)
                                                                                                                ggCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCAACCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGcc                                    >  1:76007/1‑149 (MQ=255)
                                                                                                                  cGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCt                                  <  5:281798/149‑1 (MQ=255)
                                                                                                                   gTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCtt                                 <  7:19451/149‑1 (MQ=255)
                                                                                                                    tGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTc                                <  1:212401/149‑1 (MQ=255)
                                                                                                                         aaGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCa                             <  8:157376/147‑1 (MQ=255)
                                                                                                                         aaGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGc                           >  7:299912/1‑149 (MQ=255)
                                                                                                                                   cGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCCGCCCCCCcac                   >  5:236586/1‑147 (MQ=255)
                                                                                                                                    gCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAg                <  8:299912/149‑1 (MQ=255)
                                                                                                                                          tCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgag          <  7:21198/149‑1 (MQ=255)
                                                                                                                                          tCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgag          <  6:236586/149‑1 (MQ=255)
                                                                                                                                           gtgggTGGCGGCTGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga         >  3:112663/3‑149 (MQ=255)
                                                                                                                                            aGGGTGTCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga         <  5:243655/148‑1 (MQ=255)
                                                                                                                                            aGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga         <  5:43307/148‑1 (MQ=255)
                                                                                                                                            aGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga         <  1:42386/148‑1 (MQ=255)
                                                                                                                                             gggTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGagag        >  1:95124/1‑148 (MQ=255)
                                                                                                                                                  gCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGACcgc  >  3:82499/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCCGAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGACCGC  >  NC_002947/5156180‑5156474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑