Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,156,331 C→T 6.9% R264C (CGC→TGC)  PP_4539 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,156,3310CT 6.9% 171.1 / 2.1 58R264C (CGC→TGC) PP_4539LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (25/29);  new base T (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

CGAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGACCGCGCCCA  >  NC_002947/5156183‑5156479
                                                                                                                                                    |                                                                                                                                                    
cGAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCGGGGCTTTGCCTGGCTGCCCCGTCACATGAGCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAgcc                                                                                                                                                      >  4:246205/1‑149 (MQ=255)
 gAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAgccg                                                                                                                                                     >  3:140259/1‑149 (MQ=255)
  agcagcGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAgccgc                                                                                                                                                    >  7:351435/1‑149 (MQ=255)
      gcgcTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGccacc                                                                                                                                                <  7:139113/149‑1 (MQ=255)
        gcTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccct                                                                                                                                              <  8:255752/149‑2 (MQ=255)
            gACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccctgat                                                                                                                                           <  4:158061/148‑5 (MQ=255)
            gACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCGGCTGccaccctgat                                                                                                                                           >  3:158061/1‑144 (MQ=255)
                         cTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGGGGCAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                             <  8:188513/149‑1 (MQ=255)
                            ggCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAg                                                                                                                          <  6:24130/149‑1 (MQ=255)
                             gCACCGCCGCCACCTTCGTCAGCAGCGGCCTTGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGc                                                                                                                         <  6:197958/149‑1 (MQ=255)
                               aCCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGccacc                                                                                                                                                <  7:301492/124‑1 (MQ=255)
                               aCCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGccacc                                                                                                                                                >  8:301492/1‑124 (MQ=255)
                                           ttCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCt                                                                                                           >  2:273966/1‑149 (MQ=255)
                                                   cagcGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCgg                                                                                                   >  1:82518/1‑149 (MQ=255)
                                                     gcGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGcc                                                                                                 <  3:339176/149‑1 (MQ=255)
                                                      cGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCa                                                                                                >  6:281798/1‑149 (MQ=255)
                                                               cTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGAtt                                                                                        >  2:42386/1‑148 (MQ=255)
                                                               cTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATtc                                                                                       <  8:351435/149‑1 (MQ=255)
                                                               cTTTGCCTGGCTGCCCCGCCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGGCGCTGGATCAGGGTGGCAGCCGCCACCGACTGTTCTCCCTTTATTCGAGGAAAGAGAAGACCCTGGGCCCGGCCACGAAGATtc                                                                                       >  2:56865/1‑149 (MQ=255)
                                                               cTTTGCCTGGCGGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGCAGACCCTGGGCCCGGCCACGCAGATtc                                                                                       <  2:82518/149‑1 (MQ=255)
                                                                        gCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGAc                                                                               <  4:140259/148‑1 (MQ=255)
                                                                          tGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCttt                                                                                                                                 >  7:289822/1‑96 (MQ=255)
                                                                          tGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCttt                                                                                                                                 <  8:289822/96‑1 (MQ=255)
                                                                           gCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACctgc                                                                           >  7:157376/1‑149 (MQ=255)
                                                                            ccccGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACctgc                                                                           <  4:241170/148‑1 (MQ=255)
                                                                             cccGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCCCGCAGATTCTCATCGACctgctg                                                                         >  8:21198/1‑149 (MQ=255)
                                                                              ccGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCtgctgc                                                                        >  8:19451/1‑149 (MQ=255)
                                                                               cGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCTCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTgcg                                                                       >  2:260329/1‑149 (MQ=255)
                                                                                   acaTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCCGCCGCCACCCACTGTTCTACCTGTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAAt                                                                   >  4:228224/1‑149 (MQ=255)
                                                                                    caTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGa                                                                                          >  1:131128/1‑125 (MQ=255)
                                                                                    caTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGa                                                                                          <  2:131128/125‑1 (MQ=255)
                                                                                    caTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAAtt                                                                  >  2:369376/1‑149 (MQ=255)
                                                                                     aTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAAttt                                                                 >  6:43307/1‑149 (MQ=255)
                                                                                     aTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGACAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                             >  7:188308/1‑89 (MQ=255)
                                                                                     aTGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGACAGCCGCCACCCACTGTTCTACCTTTATTc                                                                                                                             <  8:188308/89‑1 (MQ=255)
                                                                                     aTGATCGAGCGCGAGCTGCCCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATACTCAACGACCTCCTGCGAAAtt                                                                  >  6:243655/1‑148 (MQ=255)
                                                                                      tGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                                <  1:369376/149‑1 (MQ=255)
                                                                                                cgAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACCCCgcgc                                                      >  8:158862/1‑149 (MQ=255)
                                                                                                cgAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCgcgc                                                      <  3:306086/149‑1 (MQ=255)
                                                                                                  aGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCa                                                    <  8:178677/149‑1 (MQ=255)
                                                                                                   gCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCAc                                                   <  6:53186/149‑1 (MQ=255)
                                                                                                    cTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACt                                                  <  3:246205/149‑1 (MQ=255)
                                                                                                         cgAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                                >  4:219297/1‑130 (MQ=255)
                                                                                                         cgAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTc                                                                <  3:219297/130‑1 (MQ=255)
                                                                                                          gAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGt                                            >  3:309204/1‑149 (MQ=255)
                                                                                                             ggCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCAACCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGcc                                         >  1:76007/1‑149 (MQ=255)
                                                                                                               cGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCt                                       <  5:281798/149‑1 (MQ=255)
                                                                                                                gTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCtt                                      <  7:19451/149‑1 (MQ=255)
                                                                                                                 tGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTc                                     <  1:212401/149‑1 (MQ=255)
                                                                                                                      aaGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCa                                  <  8:157376/147‑1 (MQ=255)
                                                                                                                      aaGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGc                                >  7:299912/1‑149 (MQ=255)
                                                                                                                                cGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCCGCCCCCCcac                        >  5:236586/1‑147 (MQ=255)
                                                                                                                                 gCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAg                     <  8:299912/149‑1 (MQ=255)
                                                                                                                                       tCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgag               <  7:21198/149‑1 (MQ=255)
                                                                                                                                       tCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgag               <  6:236586/149‑1 (MQ=255)
                                                                                                                                        gtgggTGGCGGCTGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga              >  3:112663/3‑149 (MQ=255)
                                                                                                                                         aGGGTGTCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga              <  5:243655/148‑1 (MQ=255)
                                                                                                                                         aGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga              <  5:43307/148‑1 (MQ=255)
                                                                                                                                         aGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTgaga              <  1:42386/148‑1 (MQ=255)
                                                                                                                                          gggTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGagag             >  1:95124/1‑148 (MQ=255)
                                                                                                                                               gCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGACcgc       >  3:82499/1‑149 (MQ=255)
                                                                                                                                                    cgccACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGACCGCGCCCa  <  7:158862/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGAGCAGCGCTGGACCGTCGGCAGCCTGGGCACCGCCGCCACCTTCGTCAGCAGCGGCCTGGGCTTTGCCTGGCTGCCCCGTCACATGATCGAGCGCGAGCTGCGCGAAGGCGTGCTCAAGCCATTGCCGCTGGATCAGGGTGGCAGCCGCCACCCACTGTTCTACCTTTATTCGAGCAAAGAGAAGACCCTGGGCCCGGCCACGCAGATTCTCATCGACCTGCTGCGCAATTTCGACACCGCGCCACTGGACGTGCCCTTCGCAGCCCCCCCACAAGCCTGAGAGGACCGCGCCCA  >  NC_002947/5156183‑5156479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑