Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,221,099 T→C 7.0% H75H (CAT→CAC)  PP_4601 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,221,0990TC 7.0% 165.1 / 2.5 57H75H (CAT→CAC) PP_4601LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (26/27);  new base C (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

TATCACCCAGCCGACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTGCACTTTC  >  NC_002947/5220952‑5221238
                                                                                                                                                   |                                                                                                                                           
tatCACCCAGCCGACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCAt                                                                                                                                             >  6:308008/1‑148 (MQ=255)
    aCCCAGCCGACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACgg                                                                                                                                        <  3:125942/149‑1 (MQ=255)
     cccAGCCGACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGc                                                                                                                                       <  6:166229/149‑1 (MQ=255)
     cccAGCCGACGCTGTCCATGCGCCTGCGCAACCTGGAAGAAGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGc                                                                                                                                       >  6:94476/1‑149 (MQ=255)
         gCCGACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTg                                                                                                                                    >  7:87561/1‑148 (MQ=255)
           cGACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGGCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAg                                                                                                                                 >  5:84572/1‑149 (MQ=255)
            gACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAgg                                                                                                                                <  6:222739/149‑1 (MQ=255)
               gCTGTCCATGCGCCTGCGCCACCTGGAAGACGAACTCGACCTCGTGCTGCTCAAGCGGCGCCAGCGCTTCGAAGCTTACACCGAGCCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCg                                                                                                                             >  6:293772/1‑149 (MQ=255)
                cTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGa                                                                                                                            <  3:97935/149‑1 (MQ=255)
                  gTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAgg                                                                                                                          >  4:168052/1‑149 (MQ=255)
                    ccATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCt                                                                                                                        >  8:114355/1‑149 (MQ=255)
                     cATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTg                                                                                                                       >  1:45026/1‑149 (MQ=255)
                     cATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTg                                                                                                                       >  7:166032/1‑149 (MQ=255)
                     cATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTg                                                                                                                       >  2:101751/1‑149 (MQ=255)
                              gcgcAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGcc                                                                                                              >  5:107057/1‑149 (MQ=255)
                               cgcAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGc                                                                                                               <  5:308008/147‑1 (MQ=255)
                                gcAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGt                                                                                                            <  3:146954/149‑1 (MQ=255)
                                 cAACCTGTAAGCAGAACTGGACCTGGTGCTGGTCAAGCGGGTCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTg                                                                                                           <  5:47896/149‑1 (MQ=255)
                                        gAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAggt                                                                                                    <  3:300917/149‑1 (MQ=255)
                                         aaGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAggtg                                                                                                   <  3:89789/149‑1 (MQ=255)
                                              gAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCgg                                                                                              >  4:252624/1‑149 (MQ=255)
                                                aCTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCa                                                                                            <  3:168052/149‑1 (MQ=255)
                                                aCTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCa                                                                                            >  5:36928/1‑149 (MQ=255)
                                                        tGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGc                                                                                          >  8:239957/1‑143 (MQ=255)
                                                        tGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGc                                                                                          <  7:239957/143‑1 (MQ=255)
                                                        tGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCACGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTgcgc                                                                                    >  5:34304/1‑149 (MQ=255)
                                                        tGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCACGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTgcgc                                                                                    >  5:39791/1‑149 (MQ=255)
                                                           tGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTg                                                                                 >  3:172905/1‑149 (MQ=255)
                                                                gTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCAc                                                                            >  4:174726/1‑149 (MQ=255)
                                                                    aGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTg                                                                        <  8:166032/149‑1 (MQ=255)
                                                                          gCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTg                                                                  >  6:351922/1‑149 (MQ=255)
                                                                          gCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTg                                                                  <  2:185881/149‑1 (MQ=255)
                                                                              gcgcTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGc                                                                >  6:21936/1‑147 (MQ=255)
                                                                                gcTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGt                                                            >  6:290075/1‑149 (MQ=255)
                                                                                 cTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCGAGTTGCCGTGGCCAGGTGGTCTGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGtt                                                           <  5:94476/149‑1 (MQ=255)
                                                                                     gAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCGGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCaa                                                       <  1:20041/149‑1 (MQ=255)
                                                                                      aaGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAAc                                                      <  7:114355/149‑1 (MQ=255)
                                                                                          tttCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGtt                                                           <  7:6589/140‑1 (MQ=255)
                                                                                          tttCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGtt                                                           >  8:6589/1‑140 (MQ=255)
                                                                                           ttCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTCTCAACCCCAt                                                 >  4:131355/1‑149 (MQ=255)
                                                                                             cACCGAGGCCGGCGAGCGTATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGtt                                                           >  6:214935/1‑137 (MQ=255)
                                                                                             cACCGAGGCCGGCGAGCGTATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGtt                                                           <  5:214935/137‑1 (MQ=255)
                                                                                             cACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATg                                                <  6:107057/148‑1 (MQ=255)
                                                                                             cACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATg                                                <  5:351922/148‑1 (MQ=255)
                                                                                              aCCGAGGCCGGCCAGCGCATCCTGCCCTGGGCCCGTACCCAGCACGCCGCCCATGACGGCCAGCAGGCCGAGGCAGCCAGATCCCGTGGCCAGGTGGTCGGCACCCTCCGCCTGCGAACGATGCCGCTGGCCAGATACAACCCCATTCa                                              >  8:334061/1‑149 (MQ=255)
                                                                                                 gAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACct                                           >  4:93943/1‑149 (MQ=255)
                                                                                                            gcgcATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGcccc                                >  7:312025/1‑149 (MQ=255)
                                                                                                                      gcctggGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGaa                        <  5:293772/147‑1 (MQ=255)
                                                                                                                        ctggGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGaaaaa                     >  7:251907/1‑148 (MQ=255)
                                                                                                                           ggCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACTGTGCCGCTGGCCAGTTTCAGCCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATa                   <  4:172905/147‑1 (MQ=255)
                                                                                                                           ggCCCGTACCCTGCTCGCCGCCCACGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATAcc                 <  6:34304/149‑1 (MQ=255)
                                                                                                                           ggCCCGTACCCTGCTCGCCGCCCACGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATAcc                 <  6:39791/149‑1 (MQ=255)
                                                                                                                                    ccTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTg         >  6:62354/1‑148 (MQ=255)
                                                                                                                                           gccgccCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTGCACttt   <  2:45026/147‑1 (MQ=255)
                                                                                                                                           gccgccCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTGCACTTtc  <  5:21936/148‑1 (MQ=255)
                                                                                                                                           gccgccCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTGCACTTtc  <  4:127150/148‑1 (MQ=255)
                                                                                                                                           gccgccCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTGCACTTtc  <  5:62354/148‑1 (MQ=255)
                                                                                                                                           gccgccCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTGCACTTtc  <  6:84572/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
TATCACCCAGCCGACGCTGTCCATGCGCCTGCGCAACCTGGAAGACGAACTGGACCTGGTGCTGGTCAAGCGGGGCCAGCGCTTCGAAGGTTTCACCGAGGCCGGCGAGCGCATCCTGGCCTGGGCCCGTACCCTGCTCGCCGCCCATGACGGCCTGCAGGCCGAGGCTGCCAGTTGCCGTGGCCAGGTGGTCGGCAGCCTGCGCCTGGGCACGGTGCCGCTGGCCAGTTTCAACCCCATGCACCTGCTGCTGCCCCTGCGCGAAAAATACCCCGAACTGCACTTTC  >  NC_002947/5220952‑5221238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑