Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,289,372	T→G	6.1%	I39S (ATC→AGC)	PP_4662 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,289,372	0	T	G	6.1%	210.0 / 3.1	65	I39S (ATC→AGC)	PP_4662	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/27);  new base G (2/2);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TCGACTCACCACCCATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGAT  >  NC_002947/5289225‑5289520                                                                                                                                                    |                                                                                                                                                     tCGACTCACCACCCATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATc                                                                                                                                                     >  4:185815/1‑149 (MQ=255) tCGACTCACCACCAATCACCGAGTAGCTGCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGAGCCGACCGGAAATGGCTGATGCGTTCGAAAACCTTTTCCGTCCCTACGTCGACCCAAGCCGCCAGGAACCCGGCTTCATc                                                                                                                                                     <  5:353143/149‑1 (MQ=255)        accaccCATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTAc                                                                                                                                               >  5:98517/1‑148 (MQ=255)        accaccCATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTAc                                                                                                                                               >  4:210516/1‑148 (MQ=255)        accaccCATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATACGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTAcc                                                                                                                                              >  1:134399/1‑149 (MQ=255)             ccATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATg                                                                                                                                         <  6:65127/149‑1 (MQ=255)             ccATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATg                                                                                                                                         >  5:116208/1‑149 (MQ=255)              cATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGACCCCGGCTGCATCGAGTACCACATGt                                                                                                                                        >  6:37883/1‑149 (MQ=255)                  aCCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTgcg                                                                                                                                    >  5:275121/1‑149 (MQ=255)                             ccccATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACcagcag                                                                                                                          >  1:130038/1‑148 (MQ=255)                             ccccATGAGTGAGCCGTTAGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATTGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAAACCGGCTGCATCGAGTACCACATGTTTCGCGAccctcag                                                                                                                          >  1:125902/1‑143 (MQ=255)                                   gagtgagCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTgc                                                                                                                                     >  4:346058/1‑131 (MQ=255)                                   gagtgagCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTgc                                                                                                                                     <  3:346058/131‑1 (MQ=255)                                     gtgagCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGc                                                                                                                 >  6:331034/1‑149 (MQ=255)                                           cGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGttt                                                                                                           <  5:331034/149‑1 (MQ=255)                                                       tCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGtt                                                                                                                                       >  1:149499/1‑109 (MQ=255)                                                       tCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGtt                                                                                                                                       <  2:149499/109‑1 (MQ=255)                                                       tCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGa                                                                                                <  3:210516/148‑1 (MQ=255)                                                         cTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAgt                                                                                             >  8:63239/1‑149 (MQ=255)                                                          tCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACGCGGCTGCAGCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAgtg                                                                                            >  7:169858/1‑149 (MQ=255)                                                          tCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAg                                                                                                                  <  4:254554/127‑1 (MQ=255)                                                          tCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCAGCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAgtg                                                                                            >  7:174724/1‑149 (MQ=255)                                                          tCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAACCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCACGCCTGTTGGTGTAGTACGAAgtg                                                                                            >  8:207130/1‑149 (MQ=255)                                                           cAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGttt                                                                                                           <  4:167721/133‑1 (MQ=255)                                                           cAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGttt                                                                                                           >  3:167721/1‑133 (MQ=255)                                                            aaaGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAgtgt                                                                                           <  6:116208/148‑1 (MQ=255)                                                            aaaGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGtgtg                                                                                          >  6:213184/1‑149 (MQ=255)                                                              aGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAg                                                                                              <  1:170170/143‑1 (MQ=255)                                                              aGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAg                                                                                              >  2:170170/1‑143 (MQ=255)                                                                         gcccgGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGc                                                                                       <  3:320990/139‑1 (MQ=255)                                                                         gcccgGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGc                                                                                       >  4:320990/1‑139 (MQ=255)                                                                          cccgGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCcg                                                                            <  5:213184/149‑1 (MQ=255)                                                                                aaTGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTgg                                                                      <  3:185815/149‑1 (MQ=255)                                                                                   ggCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGTGCTGGACg                                                                   >  1:183711/1‑149 (MQ=255)                                                                                            gTTCGAAAACCTGTTCCGTCCCTACTTCGAGCCAAGCCGCCATGAACCAGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTTTTTGTGTTCTTCGAAGTTTGGGCCGACAAGGCCGCGCTGTACGTTCAATCgg                                                          <  2:125902/149‑1 (MQ=255)                                                                                            gTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCgg                                                          <  2:130038/149‑1 (MQ=255)                                                                                            gTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCgg                                                          <  6:98517/149‑1 (MQ=255)                                                                                               cGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGccc                                                       >  1:367527/1‑149 (MQ=255)                                                                                               cGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGccc                                                       >  5:107142/1‑149 (MQ=255)                                                                                                gAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCt                                                      >  5:279163/1‑149 (MQ=255)                                                                                                   aaCCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACGCGGCTGCAGCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTcc                                                   <  8:169858/149‑1 (MQ=255)                                                                                                   aaCCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCAGCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTcc                                                   <  8:174724/149‑1 (MQ=255)                                                                                                       tGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGca                                                >  5:7426/1‑148 (MQ=255)                                                                                                                 cTACGTCTAGCCAAGCCGCCATGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTg                                                                       <  8:172755/115‑1 (MQ=255)                                                                                                                 cTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCCCATGTTGCGCGCCAAGCAGGACCAAAGCCTGTTTGTGTTCTGCGAAGTGTGGGCCGACAAGGCCGCGCTg                                                                       >  7:172755/1‑115 (MQ=255)                                                                                                                 cTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCct                                     >  5:6297/1‑149 (MQ=255)                                                                                                                 cTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCct                                     <  6:279163/149‑1 (MQ=255)                                                                                                                 cTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCct                                     >  2:25190/1‑149 (MQ=255)                                                                                                                      tCGAGCCAAGCCGCCAGGAACCCGGCTGCCTCGAGTACCACATGTTGCGCGCCCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCttcttc                                <  6:271070/149‑1 (MQ=255)                                                                                                                       cGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGCCAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCttcttc                                >  2:141441/1‑148 (MQ=255)                                                                                                                        gAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGc                                                 <  4:207538/130‑1 (MQ=255)                                                                                                                        gAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGc                                                 >  3:207538/1‑130 (MQ=255)                                                                                                                           ccAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGaaaa                           <  5:37883/149‑1 (MQ=255)                                                                                                                              aGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAgcg                        >  2:217329/1‑149 (MQ=255)                                                                                                                               gccgccAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAgcgc                       <  2:367527/149‑1 (MQ=255)                                                                                                                                 cgccAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCAt                     <  6:275121/149‑1 (MQ=255)                                                                                                                                  gccAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATg                    >  8:197855/1‑149 (MQ=255)                                                                                                                                     aGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGAc                 >  4:257516/1‑149 (MQ=255)                                                                                                                                        taCCCGGGTGGATCCTGTTCCACCAGGTGCGGGCACACCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTAc              <  7:207130/148‑1 (MQ=255)                                                                                                                                        aaCCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCttcttc                                <  1:340045/131‑1 (MQ=255)                                                                                                                                        aaCCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCttcttc                                >  2:340045/1‑131 (MQ=255)                                                                                                                                        aaCCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTATTCGAAGTGTGGGCCGACAAGGCCGTGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTAc              <  2:183711/149‑1 (MQ=255)                                                                                                                                           ccGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTg           >  4:297597/1‑149 (MQ=255)                                                                                                                                              gCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGa         <  2:49458/148‑1 (MQ=255)                                                                                                                                                 gCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAgcgc     >  5:371744/1‑149 (MQ=255)                                                                                                                                                    tCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGAt  >  7:328683/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TCGACTCACCACCCATCACCGAGTAGCGCCCCATGAGTGAGCCGTTCGCCTTTATCCTCAAAGCCAAGACCCGCCCGGAAATGGCTGATGCGTTCGAAAACCTGTTCCGTCCCTACGTCGAGCCAAGCCGCCAGGAACCCGGCTGCATCGAGTACCACATGTTGCGCGACCAGCAGGACCCAAGCCTGTTTGTGTTCTTCGAAGTGTGGGCCGACAAGGCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGAT  >  NC_002947/5289225‑5289520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑