Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,336,191 T→C 8.2% V874A (GTG→GCG)  PP_4695 → two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,336,1910TC 8.2% 199.8 / 5.4 72V874A (GTG→GCG) PP_4695two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (35/31);  new base C (3/3);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATGGACCGCC  >  NC_002947/5336045‑5336334
                                                                                                                                                  |                                                                                                                                               
cAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGc                                                                                                                                               <  6:79328/149‑1 (MQ=255)
cAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGc                                                                                                                                               >  6:95439/1‑149 (MQ=255)
   cGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCATCCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGa                                                                                                                                            <  2:251499/149‑1 (MQ=255)
    gaggagCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGAt                                                                                                                                           >  6:134777/1‑149 (MQ=255)
        agCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAAc                                                                                                                                       <  2:16531/149‑1 (MQ=255)
         gCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAAcc                                                                                                                                      <  8:59504/149‑1 (MQ=255)
                  ccTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTcc                                                                                                                              >  4:188688/1‑148 (MQ=255)
                      gCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGc                                                                                                                         >  8:67884/1‑149 (MQ=255)
                          aaGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCcg                                                                                                                      <  2:51723/148‑1 (MQ=255)
                                 acaAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCt                                                                                                              >  1:343185/1‑149 (MQ=255)
                                 acaAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCt                                                                                                              >  7:253749/1‑149 (MQ=255)
                                  caAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCCTCGCCCGCGATGCCTc                                                                                                             >  4:300811/1‑149 (MQ=255)
                                       tGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTGGGCCAGGCGCTgc                                                                                                                                            >  6:23199/1‑112 (MQ=255)
                                       tGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCACCTGGGCCAGGCGCTgc                                                                                                                                            <  5:23199/113‑2 (MQ=255)
                                           aTCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCgg                                                                                                    >  1:162537/1‑149 (MQ=255)
                                              ggTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAg                                                                                                 >  3:17906/1‑149 (MQ=255)
                                                  tgctgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGcc                                                                                             >  4:183882/1‑149 (MQ=255)
                                                  tgctgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGcc                                                                                             >  4:178054/1‑149 (MQ=255)
                                                    ctgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgt                                                                                           <  3:188688/149‑1 (MQ=255)
                                                    ctgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgt                                                                                           >  4:344052/1‑149 (MQ=255)
                                                     tgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtg                                                                                          >  6:82211/1‑149 (MQ=255)
                                                     tgGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtg                                                                                          >  5:13669/1‑149 (MQ=255)
                                                       gCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgc                                                                                         <  8:78537/148‑1 (MQ=255)
                                                        cGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgcgc                                                                                       <  5:134777/149‑1 (MQ=255)
                                                               ccGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgc                                                                                         <  1:19329/140‑1 (MQ=255)
                                                               ccGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCcgtgc                                                                                         >  2:19329/1‑140 (MQ=255)
                                                                cGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCa                                                                                                                             <  6:307019/103‑1 (MQ=255)
                                                                cGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCa                                                                                                                             >  5:307019/1‑103 (MQ=255)
                                                                  ccgcAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCCGACCATTCGGACGAAGGTGACCAGCAACGCCTTGCCCAGGTTCTCATCAACCTGCTATCCAACGACCGCGATGCCTCTCCGCCCCCCAGCCCCCTGCGCGTGCGCAGc                                                                              >  2:116559/2‑148 (MQ=255)
                                                                   cgcAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGa                                                                            <  7:67884/149‑1 (MQ=255)
                                                                    gcAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAg                                                                           >  7:285685/1‑149 (MQ=255)
                                                                    gcAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAg                                                                           >  8:64427/1‑149 (MQ=255)
                                                                         ttCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTaa                                                                      >  7:63941/1‑149 (MQ=255)
                                                                         ttCGAAGTACAGTTCTTCAACCGTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTaa                                                                      >  7:55313/1‑149 (MQ=255)
                                                                                  cAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCacac                                                             >  6:141603/1‑149 (MQ=255)
                                                                                   aGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACAcc                                                            <  5:141603/149‑1 (MQ=255)
                                                                                    gTTCTTCAACCTTTGCGACCCGGAACATTGGGCCGAAGGTGACCCGCAGCTCCTGGCCCAGGTGCTGATCAACCAGCTCTCCAACGCCCGCGATGCCTGGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCg                                                           <  1:116559/149‑1 (MQ=255)
                                                                                     ttcttcAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGt                                                          >  6:137391/1‑149 (MQ=255)
                                                                                         tcAACCTTTGCGACCCGGACCATTGGGACGAAGGTGACCCGCAGCGCCAGGCCGAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGGGCAGGGAGGTTTACGAGCACACCGTCGAc                                                      >  6:148582/1‑149 (MQ=255)
                                                                                          cAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGAcc                                                     >  5:26529/1‑149 (MQ=255)
                                                                                          cAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGAc                                                      <  5:221973/148‑1 (MQ=255)
                                                                                          cAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGAc                                                      <  8:253749/148‑1 (MQ=255)
                                                                                            aCCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCt                                                    >  5:318883/1‑148 (MQ=255)
                                                                                                 tGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGAtc                                                <  5:95439/147‑1 (MQ=255)
                                                                                                       ccGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                                        >  5:50356/1‑149 (MQ=255)
                                                                                                       ccGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                                        <  3:344052/149‑1 (MQ=255)
                                                                                                         ggACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAg                                                                               >  7:119982/1‑108 (MQ=255)
                                                                                                         ggACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAg                                                                               <  8:119982/108‑1 (MQ=255)
                                                                                                         ggACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACg                                      <  8:55313/149‑1 (MQ=255)
                                                                                                         ggACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACg                                      <  4:296095/149‑1 (MQ=255)
                                                                                                          gACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGa                                     <  8:285685/149‑1 (MQ=255)
                                                                                                           aCCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGAtcgtc                                             >  4:298834/1‑140 (MQ=255)
                                                                                                           aCCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGAtcgtc                                             <  3:298834/140‑1 (MQ=255)
                                                                                                           aCCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGaa                                    <  3:178054/149‑1 (MQ=255)
                                                                                                           aCCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGaa                                    <  3:183882/149‑1 (MQ=255)
                                                                                                           aCCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGaa                                    <  4:17906/149‑1 (MQ=255)
                                                                                                             cATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAgg                                  <  5:82211/149‑1 (MQ=255)
                                                                                                               ttGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAgg                                  >  3:80680/1‑147 (MQ=255)
                                                                                                                   gCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGaa                                    >  6:117768/1‑141 (MQ=255)
                                                                                                                   gCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGaa                                    <  5:117768/141‑1 (MQ=255)
                                                                                                                     cGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGa                          >  2:28789/1‑149 (MQ=255)
                                                                                                                          gcgACCCGCAGCGCCTGGCCCAGCTGCTGACCACCCTCCTCTCCAACCCCCGCGACGCCTCGCCGCCCGCCCGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGCTCGTCGAAGACGAAGGCACCGGGATTCCg                     <  6:50356/147‑1 (MQ=255)
                                                                                                                           tGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCga                    <  1:28789/149‑1 (MQ=255)
                                                                                                                            gACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCg                     >  8:86230/1‑147 (MQ=255)
                                                                                                                               gatcAGCACCTGGGCCAGGCGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCgaagaa                >  8:297449/4‑149 (MQ=255)
                                                                                                                                 tcAGCACCTGGGCCAGGCGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGa                                        <  7:280965/122‑1 (MQ=255)
                                                                                                                                  cAGCACCTGGGCCAGGCGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCCAGGTTAACGAGCCCCCCGTCGACCTGAACGACGAAGACGAAGGCACCGGGATGCCCAAAAAca              >  7:231459/1‑148 (MQ=255)
                                                                                                                                      accTGGGCCCGGCGCTGTTCACCCTGCGCTCCACCGCCCGCGCTGCCTCGCCGCCCGGCAGCGCCGTGGGCGTGCGCAGCGTGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAAcatca           <  8:231459/146‑1 (MQ=255)
                                                                                                                                            cccAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGCTCGTCGAAGCCGAAGGCAGCGGGCTTCCGAAGAACATCATGGACCGc   >  4:202952/1‑149 (MQ=255)
                                                                                                                                             ccAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATg         >  8:114774/1‑142 (MQ=255)
                                                                                                                                             ccAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATg         <  7:114774/142‑1 (MQ=255)
                                                                                                                                             ccAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATGGACCGcc  <  2:343185/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CAGCGAGGAGCCCGTGTGCCTGGCTGAAGTGGCACAAGATGCCATCGGTCTGCTGGCGTTGAACCGGCGCAATTTCGAAGTACAGTTCTTCAACCTTTGCGACCCGGACCATTGGGCCGAAGGTGACCCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAACGCCCGCGATGCCTCGCCGCCCGGCAGCGCCGTGCGCGTGCGCAGCGAGGTTAACGAGCACACCGTCGACCTGATCGTCGAAGACGAAGGCAGCGGGATTCCGAAGAACATCATGGACCGCC  >  NC_002947/5336045‑5336334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑