Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,340,377 G→C 6.0% A76P (GCC→CCC) ‡ folK → 2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,340,3770GC 6.0% 211.2 / 2.7 66A76P (GCC→CCC) ‡folK2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base C (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

GCTGGACCAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACCACATGCATGCCCGCCCGTTCGTGCT  >  NC_002947/5340229‑5340525
                                                                                                                                                    |                                                                                                                                                    
gcTGGACCAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAgg                                                                                                                                                      <  1:101991/149‑1 (MQ=255)
    gACCAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGcc                                                                                                                                                    <  1:51367/147‑1 (MQ=255)
    gACCAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCa                                                                                                                                                   <  4:138054/148‑1 (MQ=255)
     aCCAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATc                                                                                                                                                 <  8:135952/149‑1 (MQ=255)
      ccAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCCACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATc                                                                                                                                                 >  8:12215/1‑148 (MQ=255)
      ccAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGGCCTGCAGCGCATc                                                                                                                                                 >  5:328588/1‑148 (MQ=255)
       cAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCACCCGCGCTACACCAATGCTGTCGCCGCCCTGGACACAGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCAGCg                                                                                                                                                >  1:64190/1‑148 (MQ=255)
       cAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGGCCTGCAGCGCATCGa                                                                                                                                               <  6:328588/149‑1 (MQ=255)
       cAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGGCCTGCAGCGCATCGa                                                                                                                                               <  3:231537/149‑1 (MQ=255)
          gTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCCCCACTGGCATTGCTCGATGCGCTGCAGGCCATCGaaaa                                                                                                                                            >  7:219550/1‑149 (MQ=255)
                  cACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAgg                                                                                                                                    <  6:249997/149‑1 (MQ=255)
                         cTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGt                                                                                                                             >  3:245236/1‑149 (MQ=255)
                         cTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCACCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGt                                                                                                                             >  3:292250/1‑149 (MQ=255)
                             tAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAg                                                                                                                                     >  6:105581/1‑137 (MQ=255)
                             tAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAg                                                                                                                                     <  5:105581/137‑1 (MQ=255)
                                ccgccTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCaaa                                                                                                                      <  7:164814/149‑1 (MQ=255)
                                  gccTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAaga                                                                                                                    <  3:153680/149‑1 (MQ=255)
                                  gccTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAaga                                                                                                                    <  4:347252/149‑1 (MQ=255)
                                               acacCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCACCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGccc                                                                                                        <  4:292250/148‑1 (MQ=255)
                                                 acCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCcacg                                                                                                     >  3:75639/1‑149 (MQ=255)
                                                   cAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCcacgca                                                                                                   >  5:98998/1‑149 (MQ=255)
                                                   cAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCcacgca                                                                                                   <  5:242273/149‑1 (MQ=255)
                                                    aGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCcacgcac                                                                                                  >  6:241411/1‑149 (MQ=255)
                                                    aGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCcacgcac                                                                                                  >  4:58151/1‑149 (MQ=255)
                                                      cGATTCCCTGCGGCCCTGCCAACCGCGCTACACCAATGCTGTCGGCGCCCTGGACACCGCCGTGGCACCACTCGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAATGAGCGCTGGGGCCCacgcacgc                                                                                                <  8:81199/149‑1 (MQ=255)
                                                            gctgctGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGctggac                                                                                           <  3:58151/148‑1 (MQ=255)
                                                               gctgcCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGctggacctg                                                                                        >  5:113544/1‑148 (MQ=255)
                                                                 tgcCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGcgggacctgga                                                                                      >  7:235030/1‑148 (MQ=255)
                                                                          aaCCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCctgctg                                                                            >  7:109940/1‑149 (MQ=255)
                                                                          aaCCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCctgctg                                                                            <  4:75639/149‑1 (MQ=255)
                                                                           aCCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCCCCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGt                                                                           >  8:62799/1‑149 (MQ=255)
                                                                             cgcgCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTc                                                                         >  2:277207/1‑149 (MQ=255)
                                                                                gcTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCTAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGt                                                                      <  1:277207/149‑1 (MQ=255)
                                                                                      ccAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAg                                                                <  5:241411/149‑1 (MQ=255)
                                                                                            cTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGctgg                                                                                             >  5:247657/1‑114 (MQ=255)
                                                                                            cTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGctgg                                                                                             <  6:247657/114‑1 (MQ=255)
                                                                                               tccccgccCTGGACACCGCCCTGGCACCACTGGCATTGCGCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCGACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGAc                                                       <  8:219550/146‑1 (MQ=255)
                                                                                                 gccgccCTGGACACCGCCCTGGCTCCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCa                                                     <  7:62799/149‑1 (MQ=255)
                                                                                                 gccgccCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGAcc                                                      <  7:12215/148‑1 (MQ=255)
                                                                                                  ccgccCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAg                                                    >  8:44960/1‑149 (MQ=255)
                                                                                                   cgccCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGc                                                   <  6:113544/149‑1 (MQ=255)
                                                                                                   cgccCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGc                                                   <  3:219080/149‑1 (MQ=255)
                                                                                                   cgccCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGc                                                   <  7:44960/149‑1 (MQ=255)
                                                                                                    gccCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGcc                                                  >  6:208444/1‑149 (MQ=255)
                                                                                                       cTGGACGCCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCg                                                                        <  6:176858/124‑1 (MQ=255)
                                                                                                       cTGGACGCCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCg                                                                        >  5:176858/1‑124 (MQ=255)
                                                                                                       cTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACg                                               >  7:166910/1‑149 (MQ=255)
                                                                                                                gCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACCTCCTGCTGTTCGGTGACCAGGTGCTCGACCCGCCACGCCTGAAAGt                                      >  5:64816/1‑149 (MQ=255)
                                                                                                                 cccTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGCCCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGAcc                                                      >  5:48046/1‑132 (MQ=255)
                                                                                                                 cccTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGAcc                                                      <  6:48046/132‑1 (MQ=255)
                                                                                                                 cccTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGa                                          >  7:120426/1‑144 (MQ=255)
                                                                                                                 cccTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGa                                          <  8:120426/144‑1 (MQ=255)
                                                                                                                    tGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGa                                          >  4:132836/1‑141 (MQ=255)
                                                                                                                    tGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGa                                          <  3:132836/141‑1 (MQ=255)
                                                                                                                        accacTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCctgct                                                                             <  2:79757/102‑1 (MQ=255)
                                                                                                                        accacTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCctgct                                                                             >  1:79757/1‑102 (MQ=255)
                                                                                                                          cacTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTAc                            >  5:294185/1‑149 (MQ=255)
                                                                                                                               gCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACcac                         <  6:294185/147‑1 (MQ=255)
                                                                                                                               gCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACCAcat                       <  1:24671/149‑1 (MQ=255)
                                                                                                                                    tttCGATGGCCTGCAGCGCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTGCGGTGACCAGGTGCTCGACCCGCCACGCCGGAAAGTGCCGCATTACCAcgtgcatg                  >  8:348703/3‑149 (MQ=255)
                                                                                                                                          tGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGa                                                        <  7:247663/105‑1 (MQ=255)
                                                                                                                                          tGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGa                                                        >  8:247663/1‑105 (MQ=255)
                                                                                                                                               tGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACCACATGCATGCCCGCCCGTTc       >  5:356164/1‑149 (MQ=255)
                                                                                                                                                gCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTc                                                          <  5:239962/97‑1 (MQ=255)
                                                                                                                                                gCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTc                                                          >  6:239962/1‑97 (MQ=255)
                                                                                                                                                 cAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACCACATGCATGCCCGCCCGTTc       <  6:98998/147‑1 (MQ=255)
                                                                                                                                                  aGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACCACATGCATGCCCGCCCGTTCGTg    <  6:356164/149‑1 (MQ=255)
                                                                                                                                                    gCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACCACATGCATGCCCGCCCGTTCGTGCt  >  7:246298/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGGACCAGGTCGAAGCCACGCGCCTGGTAGCCGCCTCGGCCCTGTACACCAGCGATTCGCTGCTGCCCGGCCAACCGCGCTACACCAATGCTGTCGCCGCCCTGGACACCGCCCTGGCACCACTGGCATTGCTCGATGCGCTGCAGGCCATCGAAAACGACCAGGGCCGCGTGCGCAAAGAGCGCTGGGGCCCACGCACGCTGGACCTGGACATCCTGCTGTTCGGTGACCAGGTGCTCGACCAGCCACGCCTGAAAGTGCCGCATTACCACATGCATGCCCGCCCGTTCGTGCT  >  NC_002947/5340229‑5340525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑