Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,342,608 T→A 10.2% intergenic (+54/‑153) panC → / → pgi‑II pantothenate synthetase/glucose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,342,6080TA10.2% 138.1 / 5.8 49intergenic (+54/‑153)panC/pgi‑IIpantothenate synthetase/glucose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (20/24);  new base A (2/3);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

ATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGG  >  NC_002947/5342460‑5342748
                                                                                                                                                    |                                                                                                                                            
aTGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGttt                                                                                                                                              <  2:291282/149‑1 (MQ=255)
   aTCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTAc                                                                                                                                            <  5:213714/148‑1 (MQ=255)
       agaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTa                                                                                                                                       <  5:146816/149‑1 (MQ=255)
        gaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAg                                                                                                                                      <  1:259560/149‑1 (MQ=255)
            aggtggtGATTGCGGCGGCTTACCTCGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAACAGAAGACCGCATAACGCTCTTGCGCAGCCTTGGAGCCCATTCGCGGGCAAGCCCCCTCCTTCACCGTTACCTGAACgggc                                                                                                                                  >  4:210773/2‑149 (MQ=255)
              gtggtgATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcgg                                                                                                                                <  8:208534/149‑1 (MQ=38)
                 gtgATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCACTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCATGCGGCCCAATCCCGGGCACACCCGCTCCTGGACGGGTTCCAGGAGgggcgggcg                                                                                                                             >  8:194623/1‑148 (MQ=255)
                   gATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGgcttg                                                                                                                           >  5:283870/1‑149 (MQ=255)
                    aTTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGgcttgc                                                                                                                          <  6:187099/149‑1 (MQ=255)
                       gcggcgGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGgcttgctt                                                                                                                        <  5:9108/148‑1 (MQ=255)
                          gcggcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTg                                                                                                                                         <  7:160785/128‑1 (MQ=255)
                          gcggcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCAGCACGTTTACCTg                                                                                                                                         >  8:160785/1‑128 (MQ=255)
                                    tGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCCCTACTGCACCTTTACCTGTAGGGGCGGGCTTGCTTGCAATGaccccca                                                                                                           >  6:6927/1‑146 (MQ=255)
                                      ggTACCACGCGTTTTATCGACATCCTCTACCTGGATTTTGAAGAGAAGTCGGCATAACGGTCTTTGGCTGCCTTGCAGCCCATTCGCGGGCAACCCCGCTCCTTCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCaaaa                                                                                                         <  3:210773/148‑1 (MQ=255)
                                      ggTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCaaaa                                                                                                         <  2:110241/148‑1 (MQ=255)
                                       gTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATc                                                                                                       >  1:40251/1‑149 (MQ=255)
                                         aaCACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATccc                                                                                                     >  4:203905/1‑149 (MQ=255)
                                         aaCACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGAGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcggg                                                                                                                               <  2:98946/123‑1 (MQ=37)
                                         aaCACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGAGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcggg                                                                                                                               >  1:98946/1‑123 (MQ=37)
                                            acGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCt                                                                                                  <  3:368047/149‑1 (MQ=255)
                                                    gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATAc                                                                                           <  7:194623/148‑1 (MQ=255)
                                                    gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGGAGCGGgctt                                                                                                                            <  6:268894/115‑1 (MQ=25)
                                                    gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGGAGCGGgctt                                                                                                                            >  5:268894/1‑115 (MQ=25)
                                                      tCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCa                                                                                        >  3:245315/1‑149 (MQ=255)
                                                        gACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCAtt                                                                                      <  3:221350/149‑1 (MQ=255)
                                                                   ccTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGGCCCCAAAATAACCCTGGCCTACCCCTTACCAGCACCTg                                                                           >  5:6485/1‑149 (MQ=38)
                                                                      gCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGGAGCCCATTACCGGGGAACCCCGCGCCGGCACGGTTACCTGTTTGGGCGGGCATGGTTGCAATGCCCCCCAAATACCCCGGCCAGACCCAATACCACCACCAGGcc                                                                        >  4:219590/1‑149 (MQ=255)
                                                                         tttGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGcttgcttg                                                                                                                       >  5:152386/1‑99 (MQ=255)
                                                                         tttGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGcttgcttg                                                                                                                       <  6:152386/99‑1 (MQ=255)
                                                                         tttGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCttt                                                                     <  2:40251/149‑1 (MQ=255)
                                                                               agagaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTAGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCAGCCAAACCCATTACCAGCCCGTTGGCTTTGCCta                                                                >  4:149233/1‑148 (MQ=255)
                                                                                gagaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCGCCTGCCCGTTTACCAGTATGGGCGGGCTTGCTTGCAATGCCCCCCAAATCCCCCAGCCATACCCATTCCCACCCCGTGTCCTTTGCCgat                                                               >  4:244120/1‑148 (MQ=255)
                                                                                 agaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATaa                                                             <  3:219590/149‑1 (MQ=255)
                                                                                     gaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCa                                                                                        <  3:74961/118‑1 (MQ=255)
                                                                                                 gTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAAc                                             <  4:245315/149‑1 (MQ=255)
                                                                                                      gggCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCgg                                         <  6:283870/148‑1 (MQ=255)
                                                                                                                gCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGcc                              >  7:190089/1‑149 (MQ=255)
                                                                                                                 cAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGcc                              <  3:149233/148‑1 (MQ=255)
                                                                                                                      cATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGCCTg                                >  5:7567/1‑141 (MQ=255)
                                                                                                                      cATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTg                                <  6:7567/141‑1 (MQ=255)
                                                                                                                        ttCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATAACCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCaa                      <  3:203905/149‑1 (MQ=255)
                                                                                                                          cgcgGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGc                                                                   <  7:138258/102‑1 (MQ=255)
                                                                                                                          cgcgGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGc                                                                   >  8:138258/1‑102 (MQ=255)
                                                                                                                           gcgGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGc                    <  8:190089/148‑1 (MQ=255)
                                                                                                                               gCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCTCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGcc                   >  1:201521/1‑145 (MQ=255)
                                                                                                                               gCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCTCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGcc                   <  2:201521/145‑1 (MQ=255)
                                                                                                                                   gCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTcaccac           >  6:38125/1‑149 (MQ=255)
                                                                                                                                    cccGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACg          >  8:179673/1‑149 (MQ=255)
                                                                                                                                           cTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAg   >  2:135311/1‑149 (MQ=255)
                                                                                                                                              cACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAgg  <  7:179673/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
ATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGG  >  NC_002947/5342460‑5342748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑