Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,342,611 C→G 10.4% intergenic (+57/‑150) panC → / → pgi‑II pantothenate synthetase/glucose 6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,342,6110CG10.4% 138.8 / 6.8 48intergenic (+57/‑150)panC/pgi‑IIpantothenate synthetase/glucose 6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (20/23);  new base G (2/3);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

AGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGG  >  NC_002947/5342467‑5342748
                                                                                                                                                |                                                                                                                                         
agaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTa                                                                                                                                       <  5:146816/149‑1 (MQ=255)
 gaCCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAg                                                                                                                                      <  1:259560/149‑1 (MQ=255)
     aggtggtGATTGCGGCGGCTTACCTCGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAACAGAAGACCGCATAACGCTCTTGCGCAGCCTTGGAGCCCATTCGCGGGCAAGCCCCCTCCTTCACCGTTACCTGAACgggc                                                                                                                                  >  4:210773/2‑149 (MQ=255)
       gtggtgATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcgg                                                                                                                                <  8:208534/149‑1 (MQ=38)
          gtgATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCACTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCATGCGGCCCAATCCCGGGCACACCCGCTCCTGGACGGGTTCCAGGAGgggcgggcg                                                                                                                             >  8:194623/1‑148 (MQ=255)
            gATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGgcttg                                                                                                                           >  5:283870/1‑149 (MQ=255)
             aTTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGgcttgc                                                                                                                          <  6:187099/149‑1 (MQ=255)
                gcggcgGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGgcttgctt                                                                                                                        <  5:9108/148‑1 (MQ=255)
                   gcggcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTg                                                                                                                                         <  7:160785/128‑1 (MQ=255)
                   gcggcTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCAGCACGTTTACCTg                                                                                                                                         >  8:160785/1‑128 (MQ=255)
                             tGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCCCTACTGCACCTTTACCTGTAGGGGCGGGCTTGCTTGCAATGaccccca                                                                                                           >  6:6927/1‑146 (MQ=255)
                               ggTACCACGCGTTTTATCGACATCCTCTACCTGGATTTTGAAGAGAAGTCGGCATAACGGTCTTTGGCTGCCTTGCAGCCCATTCGCGGGCAACCCCGCTCCTTCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCaaaa                                                                                                         <  3:210773/148‑1 (MQ=255)
                               ggTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCaaaa                                                                                                         <  2:110241/148‑1 (MQ=255)
                                gTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATc                                                                                                       >  1:40251/1‑149 (MQ=255)
                                  aaCACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATccc                                                                                                     >  4:203905/1‑149 (MQ=255)
                                  aaCACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGAGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcggg                                                                                                                               <  2:98946/123‑1 (MQ=37)
                                  aaCACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGAGCAAGCCCGCCCCTACAGGTAAACGTGCAGgagcggg                                                                                                                               >  1:98946/1‑123 (MQ=37)
                                     acGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCt                                                                                                  <  3:368047/149‑1 (MQ=255)
                                             gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATAc                                                                                           <  7:194623/148‑1 (MQ=255)
                                             gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGGAGCGGgctt                                                                                                                            >  5:268894/1‑115 (MQ=25)
                                             gATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCCCCTACAGGTAAACGTGCAGGAGCGGgctt                                                                                                                            <  6:268894/115‑1 (MQ=25)
                                               tCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCa                                                                                        >  3:245315/1‑149 (MQ=255)
                                                 gACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCAtt                                                                                      <  3:221350/149‑1 (MQ=255)
                                                            ccTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGGCCCCAAAATAACCCTGGCCTACCCCTTACCAGCACCTg                                                                           >  5:6485/1‑149 (MQ=38)
                                                               gCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGGAGCCCATTACCGGGGAACCCCGCGCCGGCACGGTTACCTGTTTGGGCGGGCATGGTTGCAATGCCCCCCAAATACCCCGGCCAGACCCAATACCACCACCAGGcc                                                                        >  4:219590/1‑149 (MQ=255)
                                                                  tttGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGcttgcttg                                                                                                                       >  5:152386/1‑99 (MQ=255)
                                                                  tttGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGcttgcttg                                                                                                                       <  6:152386/99‑1 (MQ=255)
                                                                  tttGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCttt                                                                     <  2:40251/149‑1 (MQ=255)
                                                                        agagaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTAGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCAGCCAAACCCATTACCAGCCCGTTGGCTTTGCCta                                                                >  4:149233/1‑148 (MQ=255)
                                                                         gagaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCGCCTGCCCGTTTACCAGTATGGGCGGGCTTGCTTGCAATGCCCCCCAAATCCCCCAGCCATACCCATTCCCACCCCGTGTCCTTTGCCgat                                                               >  4:244120/1‑148 (MQ=255)
                                                                          agaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATaa                                                             <  3:219590/149‑1 (MQ=255)
                                                                              gaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCa                                                                                        <  3:74961/118‑1 (MQ=255)
                                                                                          gTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAAc                                             <  4:245315/149‑1 (MQ=255)
                                                                                               gggCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCgg                                         <  6:283870/148‑1 (MQ=255)
                                                                                                         gCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGcc                              >  7:190089/1‑149 (MQ=255)
                                                                                                          cAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGcc                              <  3:149233/148‑1 (MQ=255)
                                                                                                               cATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGCCTg                                >  5:7567/1‑141 (MQ=255)
                                                                                                               cATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTg                                <  6:7567/141‑1 (MQ=255)
                                                                                                                 ttCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATAACCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCaa                      <  3:203905/149‑1 (MQ=255)
                                                                                                                   cgcgGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGc                                                                   <  7:138258/102‑1 (MQ=255)
                                                                                                                   cgcgGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGc                                                                   >  8:138258/1‑102 (MQ=255)
                                                                                                                    gcgGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGc                    <  8:190089/148‑1 (MQ=255)
                                                                                                                        gCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCTCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGcc                   >  1:201521/1‑145 (MQ=255)
                                                                                                                        gCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCTCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGcc                   <  2:201521/145‑1 (MQ=255)
                                                                                                                            gCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTcaccac           >  6:38125/1‑149 (MQ=255)
                                                                                                                             cccGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACg          >  8:179673/1‑149 (MQ=255)
                                                                                                                                    cTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAg   >  2:135311/1‑149 (MQ=255)
                                                                                                                                       cACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAgg  <  7:179673/147‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                         
AGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTAACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCATTCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGG  >  NC_002947/5342467‑5342748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑