Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,361,071 A→T 12.9% intergenic (‑78/+23) PP_t49 ← / ← PP_t48 tRNA‑Met/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,361,0710AT12.9% 179.8 / 15.9 70intergenic (‑78/+23)PP_t49/PP_t48tRNA‑Met/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (32/29);  new base T (5/4);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

GGGGCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTC  >  NC_002947/5360925‑5361212
                                                                                                                                                  |                                                                                                                                             
ggggCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTaaa                                                                                                                                             <  6:22028/149‑1 (MQ=255)
ggggCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTaaa                                                                                                                                             <  4:315349/149‑1 (MQ=255)
     tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAACAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                        >  7:280744/1‑149 (MQ=255)
     tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTaaa                                                                                                                                             >  8:112685/1‑144 (MQ=255)
     tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTaaa                                                                                                                                             <  7:112685/144‑1 (MQ=255)
     tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                        >  1:72901/1‑149 (MQ=255)
       gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCGACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGct                                                                                                                                      >  3:148922/1‑149 (MQ=255)
       gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGct                                                                                                                                      <  1:296631/149‑1 (MQ=255)
       gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                        <  7:282119/147‑1 (MQ=255)
         tttGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAGAGCCCGCTTTAGCGGGctt                                                                                                                                     >  3:232794/1‑147 (MQ=255)
         tttGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGctct                                                                                                                                    <  5:282293/149‑1 (MQ=255)
             aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCaa                                                                                                                                 <  8:95847/148‑1 (MQ=255)
             aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGCCAAATCCAAACATTCACCTACAAGAAAGCCCGCTTTAGCGGGCtttctt                                                                                                                                 <  1:87603/148‑3 (MQ=255)
               ccAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAGAGCCCGCTTTAGCGGGCtttcttgta                                                                                                                              <  4:232794/149‑6 (MQ=38)
                cAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCtg                                                                                                                             >  8:84103/1‑148 (MQ=255)
                 aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCtt                                                                                                                             >  4:164445/1‑148 (MQ=255)
                   cGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTaa                                                                                                                          <  5:152633/149‑1 (MQ=255)
                     aCCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAAtt                                                                                                                        <  3:164445/149‑1 (MQ=255)
                      ccTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTg                                                                                                                       <  1:195476/149‑1 (MQ=255)
                        ttCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTgg                                                                                                                      >  1:60171/1‑148 (MQ=255)
                           gggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAcc                                                                                                                  <  2:142353/149‑1 (MQ=255)
                             gTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCga                                                                                                                <  6:298200/149‑1 (MQ=255)
                             gTTATGAGCCCGACGAGCTACAAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGCGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCga                                                                                                                >  5:277901/1‑149 (MQ=255)
                                aTGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCgag                                                                                                               >  4:185701/1‑147 (MQ=255)
                                aTGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCgag                                                                                                               >  6:300412/1‑147 (MQ=255)
                                   aGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                          <  5:92323/149‑1 (MQ=255)
                                       cgacgaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                      >  6:25292/1‑149 (MQ=255)
                                        gacgaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                        >  7:138768/1‑114 (MQ=255)
                                        gacgaGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCggg                                                                                                                                        <  8:138768/114‑1 (MQ=255)
                                                 ccAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAACAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCt                                                                                             <  8:280744/148‑1 (MQ=255)
                                                    aGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGTGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGTCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                          >  3:364863/1‑132 (MQ=255)
                                                    aGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGTGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGTCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                          <  4:364863/132‑1 (MQ=255)
                                                     gctgctCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACAcc                                                                                        >  4:213925/1‑149 (MQ=255)
                                                         ctCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTa                                                                                     <  5:25292/148‑1 (MQ=255)
                                                          tCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATg                                                                                   >  4:49603/1‑149 (MQ=255)
                                                            cACCCCGCGACAAAGCTGGGGCAAAAGTATAAGCCCGAACCCTGTTGCGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGATTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACCCCCTATggg                                                                                 >  3:53902/1‑149 (MQ=255)
                                                            cACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                 >  3:233514/1‑149 (MQ=255)
                                                            cACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                 >  7:14721/1‑149 (MQ=255)
                                                               cccGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGcac                                                                              <  8:14721/149‑1 (MQ=255)
                                                                      cAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTaa                                                                                                                          >  4:173486/1‑98 (MQ=255)
                                                                      cAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTaa                                                                                                                          <  3:173486/98‑1 (MQ=255)
                                                                                gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                             >  3:162332/1‑149 (MQ=255)
                                                                                  aaaaGTATAAGACCGACCCCTGTTGAGGGTCAAATCCACACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                           <  1:124759/149‑1 (MQ=255)
                                                                                  aaaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                           >  5:5957/1‑149 (MQ=255)
                                                                                   aaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgtg                                                          >  8:280763/1‑149 (MQ=255)
                                                                                         taAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                     >  1:328051/1‑148 (MQ=255)
                                                                                         taAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                     >  7:215478/1‑148 (MQ=255)
                                                                                         taAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAACCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                     <  8:148565/148‑1 (MQ=255)
                                                                                          aaGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAg                                                                  <  3:194918/134‑1 (MQ=255)
                                                                                          aaGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAg                                                                  >  4:194918/1‑134 (MQ=255)
                                                                                           aGACCGAACCCTGTTGAGGGTCAAATACAAACATTCACCTACAAGAAATCCCGCTAAAGCGGGCTCTCAAGATTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCaa                                                  <  6:277901/149‑1 (MQ=255)
                                                                                                 aaCCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTccac                                            <  4:148922/149‑1 (MQ=255)
                                                                                                           aGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGc                                   <  3:213925/148‑1 (MQ=255)
                                                                                                             ggTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAt                                <  2:72901/149‑1 (MQ=255)
                                                                                                              gTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAta                               <  7:84103/149‑1 (MQ=255)
                                                                                                               tCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAta                               >  2:347051/1‑148 (MQ=255)
                                                                                                                       aaaCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACtt                      <  3:49603/149‑1 (MQ=255)
                                                                                                                         aCATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTg                     >  1:170577/1‑148 (MQ=255)
                                                                                                                               aCCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAccc               <  2:60171/148‑1 (MQ=255)
                                                                                                                               aCCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAccc               <  8:215478/148‑1 (MQ=255)
                                                                                                                                 cTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGt             >  7:326264/1‑148 (MQ=255)
                                                                                                                                 cTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCATTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCAACACAAGGTTCCGAGACAACCAATTCCACCACCTCCCCAATACTACTACTAGAAACCCGt             >  2:68355/1‑148 (MQ=255)
                                                                                                                                  tACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGtt            >  2:238280/1‑148 (MQ=255)
                                                                                                                                    cttgagaGCCCGCTTTAGCGGGCTTTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACt         >  4:257675/7‑149 (MQ=255)
                                                                                                                                     ttgagaGCCCGCTTTAGCGGGCTTTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACt         >  2:126308/6‑148 (MQ=255)
                                                                                                                                      aGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAcc                >  6:308341/1‑140 (MQ=255)
                                                                                                                                      aGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAcc                >  6:303225/1‑140 (MQ=255)
                                                                                                                                      aGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAcc                <  5:308341/140‑1 (MQ=255)
                                                                                                                                      aGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAAcc                <  5:303225/140‑1 (MQ=255)
                                                                                                                                      aGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAATCCACCACCTCGGCAATACTACAACTTGAAACCCGTTACtaa       >  1:331981/1‑147 (MQ=255)
                                                                                                                                         gaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGTGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGCTTGCGTGGCTACCAATTACACCACCTCCGCAATACTACTACTTGAAACCCGATAAATCTGc    >  8:128307/2‑149 (MQ=255)
                                                                                                                                         gaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATactactact                       <  3:288794/129‑1 (MQ=255)
                                                                                                                                         gaGCCCGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATactactact                       >  4:288794/2‑130 (MQ=255)
                                                                                                                                             ccGCTACAGCGTGCTCGCAAGCTGTAAGTGGTACGGCGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctc  <  4:53902/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GGGGCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCGCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAGCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTC  >  NC_002947/5360925‑5361212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑