Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	371,119	G→T	9.0%	M200I (ATG→ATT)	dgcA →	dimethylglycine dehydrogenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	371,119	0	G	T	9.0%	191.7 / 9.4	67	M200I (ATG→ATT)	dgcA	dimethylglycine dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base T (3/3);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-02

CGCAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCTACTACGACGCC  >  NC_002947/370977‑371263                                                                                                                                               |                                                                                                                                                 cgcAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTc                                                                                                                                            >  4:194093/1‑149 (MQ=255)  gcAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCACCAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCg                                                                                                                                           >  5:296340/1‑149 (MQ=255)    aGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGc                                                                                                                                         <  5:29338/149‑1 (MQ=255)           gcgTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGAATTTCATGCGGCCTTCaaa                                                                                                                                  <  8:347395/149‑2 (MQ=255)            cgTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCAACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGTCCGAATTTCATGCGGCCTTCaaag                                                                                                                                 <  3:142303/149‑3 (MQ=255)                ttgcCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTg                                                                                                                             <  6:185520/147‑1 (MQ=255)                gcgcCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTAtt                                                                                                                              <  3:249893/148‑1 (MQ=255)                gcgcCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTAtt                                                                                                                              <  6:224528/148‑1 (MQ=255)                     aaaGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGc                                                                                                                        >  7:154531/1‑149 (MQ=255)                      aaGAGGGCGGCCTGGACGGCTTGGAATTGTCGGCAGTGCACCAGCACATTATCGACCAGTTCTGGAGCCCACGGGTCAACAACCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGTAAGcc                                                                                                                       <  7:161504/149‑1 (MQ=255)                      aaGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGcc                                                                                                                       >  2:367118/1‑149 (MQ=255)                      aaGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGcc                                                                                                                       <  4:159945/149‑1 (MQ=255)                                    gACTGCTTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGaa                                                                                                           <  4:69139/147‑1 (MQ=255)                                    gACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACCAATGGGGCGGCACCTTTGAATGCCGCATGAAATTCGGCCATGAAGTAATGAAAGCCCTACTCGCCGAAGt                                                                                                         >  3:221354/1‑149 (MQ=255)                                      cGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTgg                                                                                                       <  3:194093/149‑1 (MQ=255)                                        gCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGt                                                                                                     <  1:315783/149‑1 (MQ=255)                                          gTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTga                                                                                                   <  6:251628/149‑1 (MQ=255)                                           tGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTgac                                                                                                  <  8:154531/149‑1 (MQ=255)                                                ttGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGa                                                                                                            >  5:379202/1‑134 (MQ=255)                                                ttGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGa                                                                                                            <  6:379202/134‑1 (MQ=255)                                                ttGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACtt                                                                                             >  2:77979/1‑149 (MQ=255)                                                   tCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTc                                                                                            <  2:350858/147‑1 (MQ=255)                                                   tCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTc                                                                                            >  1:350858/1‑147 (MQ=255)                                                     ggCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGc                                                                                         <  3:8603/148‑1 (MQ=255)                                                             cccAGCACATGTTCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGTGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATg                                                                                <  8:154570/148‑1 (MQ=255)                                                               cAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCg                                                                              >  4:307984/1‑149 (MQ=255)                                                                aGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACCAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCCCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCg                                                                              >  1:358409/1‑148 (MQ=255)                                                                 gCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTa                                                                            >  8:52663/1‑149 (MQ=255)                                                                     aTGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTg                                                                        <  6:86019/149‑1 (MQ=255)                                                                       gATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGAATTTCATGCGGCCTTCaaa                                                                                                                                  >  5:275139/1‑88 (MQ=37)                                                                           gACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGa                                                                  >  4:167487/1‑149 (MQ=255)                                                                            aCCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGAc                                                                 >  2:365619/1‑149 (MQ=255)                                                                             ccAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGAc                                                                 >  5:304631/1‑148 (MQ=255)                                                                               aGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGAc                                                                 >  2:328598/1‑146 (MQ=255)                                                                               aGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGAc                                                                 <  1:328598/146‑1 (MQ=255)                                                                                gTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGt                                                             >  6:48902/1‑149 (MQ=255)                                                                                 ttCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGtt                                                            >  6:7793/1‑149 (MQ=255)                                                                                   cTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTc                                                           <  7:302908/148‑1 (MQ=255)                                                                                    tGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCa                                                         >  4:66731/1‑149 (MQ=255)                                                                                      gAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTATGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCAcc                                                       >  4:125263/1‑149 (MQ=255)                                                                                            aCGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATg                                                 <  3:307984/149‑1 (MQ=255)                                                                                             cGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATgg                                                <  1:365619/149‑1 (MQ=255)                                                                                                gTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGCCGAGTTCCACCCTGATGGccc                                             >  6:239638/1‑148 (MQ=255)                                                                                                    acaaGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGc                                         >  3:206838/1‑149 (MQ=255)                                                                                                    acaaGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGc                                         <  7:52663/149‑1 (MQ=255)                                                                                                      aaGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCa                                       >  2:165836/1‑149 (MQ=255)                                                                                                       aGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCAc                                      <  1:165836/149‑1 (MQ=255)                                                                                                       aGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCAc                                      <  6:304631/149‑1 (MQ=255)                                                                                                          gTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAg                                   <  6:296340/149‑1 (MQ=255)                                                                                                             ccGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGa                                 <  3:125263/148‑1 (MQ=255)                                                                                                             ccGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGa                                 <  4:221354/148‑1 (MQ=255)                                                                                                              cgacgaATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACa                               >  4:3361/1‑149 (MQ=255)                                                                                                               gacgaATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACAt                              >  6:315184/1‑149 (MQ=255)                                                                                                                     tGGGGCGGCACCTTTGAGGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAg                          >  5:60079/1‑147 (MQ=255)                                                                                                                       gggCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGa                      <  3:66731/149‑1 (MQ=255)                                                                                                                              aCCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGAAGAATTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGAAGATCGCTGc               >  7:175856/1‑149 (MQ=255)                                                                                                                                  ttcaaGGCCGAATTTCATGCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCctac           >  4:131761/4‑149 (MQ=255)                                                                                                                                   tcaaGGCCGAATTTCATGCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACAt                              <  7:87851/127‑1 (MQ=255)                                                                                                                                   tcaaGGCCGAATTTCATGCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACAt                              >  8:87851/3‑129 (MQ=255)                                                                                                                                        gCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCTACTacgac     <  3:210873/149‑1 (MQ=255)                                                                                                                                        gCCGCATGAAATTCGGCCTTGAAGTATGGACAGCCGTACGCGCCGAAGGGGGTGACGACTTCTGCGTGGGCATGCGTAGCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCTACTacgac     <  5:139184/149‑1 (MQ=255)                                                                                                                                           gCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGcc              <  4:242452/137‑1 (MQ=255)                                                                                                                                           gCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGcc              >  3:242452/1‑137 (MQ=255)                                                                                                                                            cATGAAATTCGTCCTTGAAGTAATGAAAGACGTACGCGCCGAAGTCGGAGACGACTTCTGCGTGGGCATGCGTATCTGCGGAGACGAGTTCCACCATGATGTCCTCAGCCACGAGGACATGaa                           >  1:248788/1‑123 (MQ=255)                                                                                                                                            cATGAAATTCGGGCTTGAAGTATTGAAAGCCGTACGCGAAGAAGTGGGTGACGACTTCTGGGTGGGCATGCGTTTCTGCGGTGACGAGTTCCACCCTGTTGGCCTCAGCCACGAGGACATGaa                           <  2:248788/123‑1 (MQ=255)                                                                                                                                            cATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCTACTACGACGcc  >  3:115726/1‑148 (MQ=255)                                                                                                                                            cATGAAATTCGGCCTTCAAGTATTCAAAGCCGTACCCGCCGAAGTGGCTGACGACTTCTGCGTGGGCATGCGTATCTTCGGTGACGAGTTACACCCTGATGGCCTCAGCCAAGAGTACATCAAGCAGATCCCTGCCTACTACGACGcc  >  8:62504/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                 CGCAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCTACTACGACGCC  >  NC_002947/370977‑371263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑