Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,388,883	T→G	5.2%	L798R (CTG→CGG)	dld2 →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,388,883	0	T	G	5.2%	220.4 / 2.0	71	L798R (CTG→CGG)	dld2	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (31/36);  new base G (2/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGC  >  NC_002947/5388735‑5389025                                                                                                                                                      |                                                                                                                                               aGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCACCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCt                                                                                                                                                <  4:39892/149‑1 (MQ=255)  gCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCTGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTg                                                                                                                                               <  3:105015/149‑1 (MQ=255)    cTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGt                                                                                                                                             >  6:44376/1‑149 (MQ=255)    cTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGt                                                                                                                                             <  6:127575/149‑1 (MQ=255)       tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTg                                                                                                                                            <  2:38518/147‑1 (MQ=255)               ccAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCt                                                                                                                                    >  1:195525/1‑147 (MQ=255)                cAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAg                                                                                                                                 >  5:47678/1‑149 (MQ=255)                cAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAg                                                                                                                                 <  8:224693/149‑1 (MQ=255)                          ccTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCgg                                                                                                                       >  5:34516/1‑149 (MQ=255)                           cTCCAAGGGCTACCCCGATCAGGCCGACCACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCAGGCGCAGGGTGCAggg                                                                                                                                       <  1:211035/132‑2 (MQ=255)                           cTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGCCACAAGCCCCTGC‑CCCAGGCGCAGGGTGCAggg                                                                                                                                       >  2:216632/1‑131 (MQ=255)                           cTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCAGGCGCAGGGGGCAggg                                                                                                                                       <  1:216632/132‑2 (MQ=255)                           cTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGAGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCAGGCGCAGGGTGCAggg                                                                                                                                       >  2:211035/1‑131 (MQ=255)                            tCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                     >  7:182697/1‑149 (MQ=255)                            tCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                     >  7:59362/1‑149 (MQ=255)                            tCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                     >  6:251464/1‑149 (MQ=255)                              cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTgg                                                                                                                   <  5:81541/149‑1 (MQ=255)                              cAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                    <  2:195525/148‑1 (MQ=255)                               aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGaca                                                                                                                            >  7:34200/1‑139 (MQ=255)                               aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGaca                                                                                                                            <  8:34200/139‑1 (MQ=255)                               aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                  <  8:228384/149‑1 (MQ=255)                               aaGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                  <  4:61623/149‑1 (MQ=255)                                aGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                 <  5:31489/149‑1 (MQ=255)                                aGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                 <  1:144016/149‑1 (MQ=255)                                        cccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGa                                                                                                         >  1:229363/1‑149 (MQ=255)                                         ccGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAc                                                                                                        >  7:54925/1‑149 (MQ=255)                                              cAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGt                                                                                                   <  5:251464/149‑1 (MQ=255)                                               aGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                  <  6:47678/149‑1 (MQ=255)                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                     <  8:163581/144‑1 (MQ=255)                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                     >  7:163581/1‑144 (MQ=255)                                                 gCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcg                                                                                                >  8:18385/1‑149 (MQ=255)                                                        acaAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATc                                                                                         <  7:18385/149‑1 (MQ=255)                                                        acaAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATc                                                                                         >  7:159608/1‑149 (MQ=255)                                                            aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                     >  6:204902/1‑149 (MQ=255)                                                                cAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcaccca                                                                                 >  8:305711/1‑149 (MQ=255)                                                                 aGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                                <  8:159608/149‑1 (MQ=255)                                                                        gATCACCGCCCTGCTGCCCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACCCCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcg                                                                         >  5:15543/1‑149 (MQ=255)                                                                         aTCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTc                                                                          <  4:104163/147‑1 (MQ=255)                                                                           cACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgccc                                                                      >  3:94259/1‑149 (MQ=255)                                                                            aCCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCGGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgacc                                                                      <  6:89810/148‑1 (MQ=255)                                                                             ccGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCa                                                                                           <  5:286175/126‑1 (MQ=255)                                                                             ccGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCa                                                                                           >  6:286175/1‑126 (MQ=255)                                                                                cccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                 <  7:229296/149‑1 (MQ=255)                                                                                 ccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                 >  7:285503/1‑148 (MQ=255)                                                                                   tgctgcACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAg                                                              >  4:275649/1‑149 (MQ=255)                                                                                        cACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCa                                                          <  8:59362/148‑1 (MQ=255)                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCGCCCCTGCGCCTGGTGCAGGACCTGAGCGCCACCCGGCTGGACCGGTACGACCCCGTGCGCTTCATCCGCCCCCACCTGCGCGACCGCCTGGAGTTCAccc                                                       >  1:271613/1‑149 (MQ=255)                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGaca                                                                                                                            >  8:317721/1‑79 (MQ=255)                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGaca                                                                                                                            <  7:317721/79‑1 (MQ=255)                                                                                           gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcccc                                                      >  7:105301/1‑149 (MQ=255)                                                                                              aGCCGCGGCGGCCACGACCCGAGCTATTGCGACACACGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCa                                                   <  8:182697/149‑1 (MQ=255)                                                                                               gCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAg                                                  <  5:204902/149‑1 (MQ=255)                                                                                                     gcggcCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGaa                                            <  3:275649/149‑1 (MQ=255)                                                                                                      cggcCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                           <  8:54925/149‑1 (MQ=255)                                                                                                            cGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGc                                                                                                                                <  3:134341/58‑1 (MQ=255)                                                                                                            cGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGc                                                                                                                                >  4:134341/1‑58 (MQ=255)                                                                                                             gACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                     >  2:98420/1‑100 (MQ=255)                                                                                                             gACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                     <  1:98420/100‑1 (MQ=255)                                                                                                                 cGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                        >  6:317329/1‑141 (MQ=255)                                                                                                                 cGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                        <  5:317329/141‑1 (MQ=255)                                                                                                                       aTTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCCGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                           >  3:35581/1‑148 (MQ=255)                                                                                                                        ttGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCAACCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCAc                             <  6:34516/145‑1 (MQ=255)                                                                                                                          gCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAc                        <  2:229363/148‑1 (MQ=255)                                                                                                                           cGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCt                      <  7:305711/149‑1 (MQ=255)                                                                                                                           cGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCCGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAcc                       <  4:35581/148‑1 (MQ=255)                                                                                                                           cGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCCGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAcc                       <  5:44376/148‑1 (MQ=255)                                                                                                                               acaAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGCCCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcag                  >  8:100366/1‑149 (MQ=255)                                                                                                                                 aaGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                        >  3:276062/1‑125 (MQ=255)                                                                                                                                 aaGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCgg                                        <  4:276062/125‑1 (MQ=255)                                                                                                                                              ccTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCCGCACCTgg    >  6:231819/1‑149 (MQ=255)                                                                                                                                               cTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg   <  2:312028/149‑1 (MQ=255)                                                                                                                                                tGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGc  >  4:243228/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                               AGCCTGTGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACAAACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGC‑ACCCTGCGCCTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGGC  >  NC_002947/5388735‑5389025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑