Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,407,046 T→G 6.7% N124T (AAC→ACC)  PP_4751 ← amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,407,0460TG 6.7% 183.8 / 3.9 60N124T (AAC→ACC) PP_4751amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (30/25);  new base G (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GCGATACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCG  >  NC_002947/5406899‑5407190
                                                                                                                                                    |                                                                                                                                                
gcgatACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGtt                                                                                                                                                 >  3:144473/1‑149 (MQ=255)
  gatACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTgc                                                                                                                                               >  7:148713/1‑149 (MQ=255)
    tACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTgcgc                                                                                                                                             <  4:204908/149‑1 (MQ=255)
      cGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgca                                                                                                                                            <  8:128132/148‑1 (MQ=255)
       gTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgcag                                                                                                                                           >  3:245707/1‑148 (MQ=255)
       gTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgcag                                                                                                                                           >  2:175325/1‑148 (MQ=255)
            tgctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGt                                                                                                                                     >  4:237573/1‑149 (MQ=255)
              ctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGt                                                                                                                                     >  4:229250/1‑147 (MQ=255)
               tgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAg                                                                                                                                  >  4:896/1‑149 (MQ=255)
                 gacaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACTCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAgg                                                                                                                                 <  7:226524/146‑1 (MQ=255)
                  ccaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTg                                                                                                                               <  3:896/149‑1 (MQ=255)
                  ccaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGCCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgcct                                                                                                                               <  8:156982/149‑4 (MQ=255)
                   caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAg                                                                                                                                  <  5:49993/145‑1 (MQ=255)
                   caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAg                                                                                                                                  >  6:49993/1‑145 (MQ=255)
                     ccaGAGCAGCGCGCCGGATAATGCTGGGGCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                            <  4:304058/149‑1 (MQ=255)
                     ccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                            <  4:245707/149‑1 (MQ=255)
                     ccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCAAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                            >  5:243554/1‑149 (MQ=255)
                        gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAcc                                                                                                                                       <  4:102416/135‑1 (MQ=255)
                        gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAcc                                                                                                                                       >  3:102416/1‑135 (MQ=255)
                         agcagcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAAc                                                                                                                        <  2:283423/149‑1 (MQ=255)
                            agcGCGCCGGATAATGCTGGGCCTTGTCCGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGtt                                                                                                                                                 <  7:142300/121‑1 (MQ=255)
                            agcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGtt                                                                                                                                                 >  6:329825/1‑121 (MQ=255)
                            agcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGtt                                                                                                                                                 <  5:329825/121‑1 (MQ=255)
                                 gcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGaa                                                                                                                <  3:237573/149‑1 (MQ=255)
                                  ccGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAAc                                                                                                               <  8:148713/149‑1 (MQ=255)
                                         aTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                         >  6:128438/1‑148 (MQ=255)
                                         aTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                         <  2:143344/148‑1 (MQ=255)
                                            cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                      >  5:57633/1‑132 (MQ=255)
                                            cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                      <  6:57633/132‑1 (MQ=255)
                                            cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAaca                                                                                                     <  4:144473/149‑1 (MQ=255)
                                             tGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGa                                                                                                                          <  3:161606/127‑1 (MQ=255)
                                             tGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGa                                                                                                                          >  4:161606/1‑127 (MQ=255)
                                               ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAacac                                                                                                    <  2:35439/147‑1 (MQ=255)
                                                  cTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCGGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATAc                                                                                               <  4:261538/149‑1 (MQ=255)
                                                        agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                         >  7:305504/1‑149 (MQ=255)
                                                        agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAACGTCAGGTGCGGGAACAGGGTGAACGACTGGAACACCATACCAATgc                                                                                         >  4:268453/1‑149 (MQ=255)
                                                         gccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCATGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                         >  1:286079/1‑149 (MQ=255)
                                                         gccagccCAACGCGTACGATCAGTAGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGATGCGACGCACCGTCAGGTGCGGGAACAGCTTGAACGACAGGAACACCATAcccaaacg                                                                                        >  2:339435/1‑143 (MQ=255)
                                                                 aaCGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGgcgc                                                                                 >  7:58993/1‑148 (MQ=255)
                                                                       ttCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACtt                                                                            >  3:333009/1‑147 (MQ=255)
                                                                          gAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTcag                                                                         >  5:169609/1‑147 (MQ=255)
                                                                               gTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCt                                                                  <  6:169609/149‑1 (MQ=255)
                                                                                  gCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTa                                                               >  5:365634/1‑149 (MQ=255)
                                                                                  gCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTa                                                               >  2:261976/1‑149 (MQ=255)
                                                                                    aaGGAGCGGGCGTCTGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACg                                                             >  7:315998/1‑149 (MQ=255)
                                                                                                   gCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                               >  2:219020/1‑148 (MQ=255)
                                                                                                     ttcttTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                            >  4:166263/1‑149 (MQ=255)
                                                                                                        tttGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCtt                                         >  2:10072/1‑149 (MQ=255)
                                                                                                          tGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCt                                          <  8:305504/146‑1 (MQ=255)
                                                                                                          tGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTc                                        >  1:278002/1‑148 (MQ=255)
                                                                                                            gcgc‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACAATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                     <  6:243554/149‑1 (MQ=255)
                                                                                                                       ccAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGcca                           <  6:365634/149‑1 (MQ=255)
                                                                                                                           ggTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCTAGCTTCGATGTGCACGccatcca                       <  1:175325/149‑1 (MQ=255)
                                                                                                                             tGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTTAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATg                     >  6:188401/1‑149 (MQ=255)
                                                                                                                                ggATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCt                   >  6:297133/1‑148 (MQ=255)
                                                                                                                                 gttttGCGCCAGGCTGAAGTTGCGCAGCACCGTCATGTGCGGGTACAGGTTGAACGAATGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGa                 <  3:268453/147‑1 (MQ=255)
                                                                                                                                 gATTTGCGCCAGTCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGTTTGAACGACTGGAACACCATACGAATGCGCCGGGGCAACTTCAGCAGTTCTGTACGGGGCAACGCATTGCCAGCTTCGATGTGCACGCCATCCATGCTGa                 <  1:339435/149‑1 (MQ=255)
                                                                                                                                       cgcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGcc           >  1:150282/1‑149 (MQ=255)
                                                                                                                                        gcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGccc          <  2:286079/149‑1 (MQ=255)
                                                                                                                                            ggCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTg      <  5:128438/149‑1 (MQ=255)
                                                                                                                                            ggCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTg      >  4:189445/1‑149 (MQ=255)
                                                                                                                                             gCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAATGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTgg     >  1:40671/1‑149 (MQ=255)
                                                                                                                                              cTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGt    >  4:196569/1‑149 (MQ=255)
                                                                                                                                                gACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTcg  >  2:42795/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCGATACGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCG  >  NC_002947/5406899‑5407190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑