Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,407,052	A→C	8.5%	L122R (CTG→CGG) ‡	PP_4751 ←	amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,407,052	0	A	C	8.5%	164.3 / 5.5	57	L122R (CTG→CGG) ‡	PP_4751	amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (27/25);  new base C (3/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

CGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAG  >  NC_002947/5406905‑5407200                                                                                                                                                     |                                                                                                                                                     cGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgca                                                                                                                                                      <  8:128132/148‑1 (MQ=255)  gTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgcag                                                                                                                                                     >  3:245707/1‑148 (MQ=255)  gTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgcag                                                                                                                                                     >  2:175325/1‑148 (MQ=255)       tgctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGt                                                                                                                                               >  4:237573/1‑149 (MQ=255)         ctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGt                                                                                                                                               >  4:229250/1‑147 (MQ=255)          tgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAg                                                                                                                                            >  4:896/1‑149 (MQ=255)            gacaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACTCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAgg                                                                                                                                           <  7:226524/146‑1 (MQ=255)             ccaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTg                                                                                                                                         <  3:896/149‑1 (MQ=255)             ccaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGCCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgcct                                                                                                                                         <  8:156982/149‑4 (MQ=255)              caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAg                                                                                                                                            <  5:49993/145‑1 (MQ=255)              caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAg                                                                                                                                            >  6:49993/1‑145 (MQ=255)                ccaGAGCAGCGCGCCGGATAATGCTGGGGCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                      <  4:304058/149‑1 (MQ=255)                ccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                      <  4:245707/149‑1 (MQ=255)                ccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCAAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                      >  5:243554/1‑149 (MQ=255)                   gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAcc                                                                                                                                                 >  3:102416/1‑135 (MQ=255)                   gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAcc                                                                                                                                                 <  4:102416/135‑1 (MQ=255)                    agcagcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAAc                                                                                                                                  <  2:283423/149‑1 (MQ=255)                            gcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGaa                                                                                                                          <  3:237573/149‑1 (MQ=255)                             ccGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAAc                                                                                                                         <  8:148713/149‑1 (MQ=255)                                    aTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                                   <  2:143344/148‑1 (MQ=255)                                    aTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                                   >  6:128438/1‑148 (MQ=255)                                       cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                                >  5:57633/1‑132 (MQ=255)                                       cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                                <  6:57633/132‑1 (MQ=255)                                       cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAaca                                                                                                               <  4:144473/149‑1 (MQ=255)                                        tGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGa                                                                                                                                    >  4:161606/1‑127 (MQ=255)                                        tGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGa                                                                                                                                    <  3:161606/127‑1 (MQ=255)                                          ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAacac                                                                                                              <  2:35439/147‑1 (MQ=255)                                             cTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCGGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATAc                                                                                                         <  4:261538/149‑1 (MQ=255)                                                   agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                                   >  7:305504/1‑149 (MQ=255)                                                   agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAACGTCAGGTGCGGGAACAGGGTGAACGACTGGAACACCATACCAATgc                                                                                                   >  4:268453/1‑149 (MQ=255)                                                    gccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCATGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                                   >  1:286079/1‑149 (MQ=255)                                                    gccagccCAACGCGTACGATCAGTAGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGATGCGACGCACCGTCAGGTGCGGGAACAGCTTGAACGACAGGAACACCATAcccaaacg                                                                                                  >  2:339435/1‑143 (MQ=255)                                                            aaCGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGgcgc                                                                                           >  7:58993/1‑148 (MQ=255)                                                                  ttCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACtt                                                                                      >  3:333009/1‑147 (MQ=255)                                                                     gAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTcag                                                                                   >  5:169609/1‑147 (MQ=255)                                                                          gTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCt                                                                            <  6:169609/149‑1 (MQ=255)                                                                             gCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTa                                                                         >  2:261976/1‑149 (MQ=255)                                                                             gCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTa                                                                         >  5:365634/1‑149 (MQ=255)                                                                               aaGGAGCGGGCGTCTGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACg                                                                       >  7:315998/1‑149 (MQ=255)                                                                                              gCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                                         >  2:219020/1‑148 (MQ=255)                                                                                                ttcttTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                                      >  4:166263/1‑149 (MQ=255)                                                                                                   tttGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCtt                                                   >  2:10072/1‑149 (MQ=255)                                                                                                     tGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCt                                                    <  8:305504/146‑1 (MQ=255)                                                                                                     tGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTc                                                  >  1:278002/1‑148 (MQ=255)                                                                                                       gcgc‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACAATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                               <  6:243554/149‑1 (MQ=255)                                                                                                                  ccAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGcca                                     <  6:365634/149‑1 (MQ=255)                                                                                                                      ggTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCTAGCTTCGATGTGCACGccatcca                                 <  1:175325/149‑1 (MQ=255)                                                                                                                        tGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTTAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATg                               >  6:188401/1‑149 (MQ=255)                                                                                                                           ggATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCt                             >  6:297133/1‑148 (MQ=255)                                                                                                                            gttttGCGCCAGGCTGAAGTTGCGCAGCACCGTCATGTGCGGGTACAGGTTGAACGAATGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGa                           <  3:268453/147‑1 (MQ=255)                                                                                                                            gATTTGCGCCAGTCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGTTTGAACGACTGGAACACCATACGAATGCGCCGGGGCAACTTCAGCAGTTCTGTACGGGGCAACGCATTGCCAGCTTCGATGTGCACGCCATCCATGCTGa                           <  1:339435/149‑1 (MQ=255)                                                                                                                                  cgcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGcc                     >  1:150282/1‑149 (MQ=255)                                                                                                                                   gcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGccc                    <  2:286079/149‑1 (MQ=255)                                                                                                                                       ggCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTg                <  5:128438/149‑1 (MQ=255)                                                                                                                                       ggCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTg                >  4:189445/1‑149 (MQ=255)                                                                                                                                        gCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAATGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTgg               >  1:40671/1‑149 (MQ=255)                                                                                                                                         cTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGt              >  4:196569/1‑149 (MQ=255)                                                                                                                                           gACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTcg            >  2:42795/1‑149 (MQ=255)                                                                                                                                                  cgcAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTc     <  5:297133/149‑1 (MQ=255)                                                                                                                                                  cgcAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTc     <  8:315998/149‑1 (MQ=255)                                                                                                                                                    cagcaCCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGACGTGCACGCCATCCATGCCGACCCGCCCCTTGGTCGGCGGCTCCa   <  1:10072/149‑1 (MQ=255)                                                                                                                                                     agcaCCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAg  >  8:207608/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAG  >  NC_002947/5406905‑5407200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑