Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,407,056 C→A 9.7% V121L (GTG→TTG)  PP_4751 ← amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,407,0560CA 9.7% 172.6 / 7.6 59V121L (GTG→TTG) PP_4751amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base A (3/3);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAGTTGG  >  NC_002947/5406911‑5407204
                                                                                                                                                  |                                                                                                                                                    
tgctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGt                                                                                                                                                   >  4:237573/1‑149 (MQ=255)
  ctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGt                                                                                                                                                   >  4:229250/1‑147 (MQ=255)
   tgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAg                                                                                                                                                >  4:896/1‑149 (MQ=255)
     gacaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACTCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAgg                                                                                                                                               <  7:226524/146‑1 (MQ=255)
      ccaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTg                                                                                                                                             <  3:896/149‑1 (MQ=255)
      ccaccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGCCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgcct                                                                                                                                             <  8:156982/149‑4 (MQ=255)
       caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAg                                                                                                                                                >  6:49993/1‑145 (MQ=255)
       caccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAg                                                                                                                                                <  5:49993/145‑1 (MQ=255)
         ccaGAGCAGCGCGCCGGATAATGCTGGGGCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                          <  4:304058/149‑1 (MQ=255)
         ccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                          <  4:245707/149‑1 (MQ=255)
         ccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCAAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                                          >  5:243554/1‑149 (MQ=255)
            gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAcc                                                                                                                                                     <  4:102416/135‑1 (MQ=255)
            gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAcc                                                                                                                                                     >  3:102416/1‑135 (MQ=255)
             agcagcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAAc                                                                                                                                      <  2:283423/149‑1 (MQ=255)
                     gcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGaa                                                                                                                              <  3:237573/149‑1 (MQ=255)
                      ccGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAAc                                                                                                                             <  8:148713/149‑1 (MQ=255)
                             aTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                                       <  2:143344/148‑1 (MQ=255)
                             aTGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTgg                                                                                                                       >  6:128438/1‑148 (MQ=255)
                                cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                                    >  5:57633/1‑132 (MQ=255)
                                cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAg                                                                                                                                    <  6:57633/132‑1 (MQ=255)
                                cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAaca                                                                                                                   <  4:144473/149‑1 (MQ=255)
                                 tGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGa                                                                                                                                        <  3:161606/127‑1 (MQ=255)
                                 tGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGa                                                                                                                                        >  4:161606/1‑127 (MQ=255)
                                   ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAacac                                                                                                                  <  2:35439/147‑1 (MQ=255)
                                      cTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCGGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATAc                                                                                                             <  4:261538/149‑1 (MQ=255)
                                            agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                                       >  7:305504/1‑149 (MQ=255)
                                            agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAACGTCAGGTGCGGGAACAGGGTGAACGACTGGAACACCATACCAATgc                                                                                                       >  4:268453/1‑149 (MQ=255)
                                             gccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCATGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                                       >  1:286079/1‑149 (MQ=255)
                                             gccagccCAACGCGTACGATCAGTAGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGATGCGACGCACCGTCAGGTGCGGGAACAGCTTGAACGACAGGAACACCATAcccaaacg                                                                                                      >  2:339435/1‑143 (MQ=255)
                                                     aaCGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGgcgc                                                                                               >  7:58993/1‑148 (MQ=255)
                                                           ttCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACtt                                                                                          >  3:333009/1‑147 (MQ=255)
                                                              gAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTcag                                                                                       >  5:169609/1‑147 (MQ=255)
                                                                   gTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCt                                                                                <  6:169609/149‑1 (MQ=255)
                                                                      gCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTa                                                                             >  5:365634/1‑149 (MQ=255)
                                                                      gCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTa                                                                             >  2:261976/1‑149 (MQ=255)
                                                                        aaGGAGCGGGCGTCTGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACg                                                                           >  7:315998/1‑149 (MQ=255)
                                                                                       gCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                                             >  2:219020/1‑148 (MQ=255)
                                                                                         ttcttTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                                          >  4:166263/1‑149 (MQ=255)
                                                                                            tttGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCtt                                                       >  2:10072/1‑149 (MQ=255)
                                                                                              tGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCt                                                        <  8:305504/146‑1 (MQ=255)
                                                                                              tGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTc                                                      >  1:278002/1‑148 (MQ=255)
                                                                                                gcgc‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACAATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                                   <  6:243554/149‑1 (MQ=255)
                                                                                                           ccAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGcca                                         <  6:365634/149‑1 (MQ=255)
                                                                                                               ggTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCTAGCTTCGATGTGCACGccatcca                                     <  1:175325/149‑1 (MQ=255)
                                                                                                                 tGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTTAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATg                                   >  6:188401/1‑149 (MQ=255)
                                                                                                                    ggATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCt                                 >  6:297133/1‑148 (MQ=255)
                                                                                                                     gttttGCGCCAGGCTGAAGTTGCGCAGCACCGTCATGTGCGGGTACAGGTTGAACGAATGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGa                               <  3:268453/147‑1 (MQ=255)
                                                                                                                     gATTTGCGCCAGTCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGTTTGAACGACTGGAACACCATACGAATGCGCCGGGGCAACTTCAGCAGTTCTGTACGGGGCAACGCATTGCCAGCTTCGATGTGCACGCCATCCATGCTGa                               <  1:339435/149‑1 (MQ=255)
                                                                                                                           cgcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGcc                         >  1:150282/1‑149 (MQ=255)
                                                                                                                            gcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGccc                        <  2:286079/149‑1 (MQ=255)
                                                                                                                                ggCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTg                    <  5:128438/149‑1 (MQ=255)
                                                                                                                                ggCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTg                    >  4:189445/1‑149 (MQ=255)
                                                                                                                                 gCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAATGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTgg                   >  1:40671/1‑149 (MQ=255)
                                                                                                                                  cTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGt                  >  4:196569/1‑149 (MQ=255)
                                                                                                                                    gACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTcg                >  2:42795/1‑149 (MQ=255)
                                                                                                                                         tgctgcgcaACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCggcggc           <  3:196569/143‑1 (MQ=255)
                                                                                                                                           cgcAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTc         <  5:297133/149‑1 (MQ=255)
                                                                                                                                           cgcAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTc         <  8:315998/149‑1 (MQ=255)
                                                                                                                                             cagcaCCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGACGTGCACGCCATCCATGCCGACCCGCCCCTTGGTCGGCGGCTCCa       <  1:10072/149‑1 (MQ=255)
                                                                                                                                              agcaCCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAg      >  8:207608/1‑149 (MQ=255)
                                                                                                                                                caCCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAgtt    <  7:207608/149‑1 (MQ=255)
                                                                                                                                                  ccGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCTAgttgg  <  8:58993/149‑1 (MQ=255)
                                                                                                                                                  ccGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAgttgg  <  2:278002/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGC‑TGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCGGCTCCAGTTGG  >  NC_002947/5406911‑5407204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑