Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,426,091 G→C 9.0% W209C (TGG→TGC) ‡ PP_4765 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,426,0910GC 9.0% 237.9 / 11.5 77W209C (TGG→TGC) ‡PP_4765hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/33);  new base C (3/4);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TCGGTCGTGGTGCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCACCGGT  >  NC_002947/5425951‑5426237
                                                                                                                                            |                                                                                                                                                  
tCGGTCGTGGTGCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAgcgcgc                                                                                                                                             >  3:255372/1‑148 (MQ=255)
 cGGTCGTGGTGCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTa                                                                                                                                           <  4:255372/149‑1 (MQ=255)
   gTCGGGGGGCTGGCGCGGGGTGGTGCCAGCTGTGCGCGCCTGGGGTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCGGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACt                                                                                                                                         <  3:229745/149‑1 (MQ=255)
       tggtgCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGCGCTCCAGCCCGCTACttcaa                                                                                                                                     <  6:71403/149‑5 (MQ=255)
         gtgCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAgg                                                                                                                                    <  7:90269/148‑1 (MQ=255)
          tgCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGa                                                                                                                                   >  4:78163/1‑148 (MQ=255)
          tgCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGa                                                                                                                                   >  7:2396/1‑148 (MQ=255)
             tGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAg                                                                                                                               >  7:95588/1‑149 (MQ=255)
                 gcTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTcg                                                                                                                           >  5:19149/1‑149 (MQ=255)
                   tGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTcgcg                                                                                                                         >  8:62727/1‑149 (MQ=255)
                   tGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGCGCTCCAGCCCGCTACttcaaaa                                                                                                                                   >  1:161791/1‑133 (MQ=255)
                   tGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGCGCTCCAGCCCGCTACttcaaaa                                                                                                                                   <  2:161791/139‑7 (MQ=255)
                   tGGGTGGTGCCAGCTGGGAGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGAAACTGCGGTTTTGAAGTAGCGGGCTGGCGCGCGCTACTCAAGGACAAGTTcgcg                                                                                                                         >  8:200820/1‑149 (MQ=255)
                          tgCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCAc                                                                                                                  <  6:84819/149‑1 (MQ=255)
                          tgCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCAc                                                                                                                  >  2:184933/1‑149 (MQ=255)
                                   ggCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTgaaga                                                                                                         <  2:154823/149‑1 (MQ=255)
                                    gcgcgcCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTgaagaa                                                                                                        <  1:184933/149‑1 (MQ=255)
                                    gcgcgcCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTgaagaa                                                                                                        <  6:131037/149‑1 (MQ=255)
                                              gTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCt                                                                                               >  3:183475/1‑148 (MQ=255)
                                                   gACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGt                                                                                         >  5:36851/1‑149 (MQ=255)
                                                    aCGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTc                                                                                        >  8:37893/1‑149 (MQ=255)
                                                          gCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCggg                                                                                   >  2:283543/1‑148 (MQ=255)
                                                           cTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCa                                                                                 <  4:183475/149‑1 (MQ=255)
                                                              gCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAgcg                                                                              >  2:363728/1‑149 (MQ=255)
                                                              gCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAg                                                                                <  7:62727/147‑1 (MQ=255)
                                                               cAACCGCTGCTGGCAGAACGGGGTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGAGGTTTTGAAGTAGCGGGCTGGATAGCGATACTCAATGACAAGTTCGCGTGCTCACAGCTGAAGAAAATCTCCCTAAGCTTCACCTGCAgcg                                                                              >  7:135939/1‑148 (MQ=255)
                                                                 aCCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCAc                                                                           >  5:3600/1‑149 (MQ=255)
                                                                      tgctgGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACcgcg                                                                       >  5:167242/1‑148 (MQ=255)
                                                                        ctgGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCgcgc                                                                      >  8:300424/1‑147 (MQ=255)
                                                                         tgGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAg                                                                   >  6:98315/1‑149 (MQ=255)
                                                                           gCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAggg                                                                 >  4:211878/1‑149 (MQ=255)
                                                                            cAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGc                                                                <  1:363728/149‑1 (MQ=255)
                                                                              gAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGa                                                              <  7:24469/149‑1 (MQ=255)
                                                                              gAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGa                                                              <  8:95588/149‑1 (MQ=255)
                                                                               aaCGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAg                                                             >  6:120265/1‑149 (MQ=255)
                                                                                      tgtgGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAg                                                             >  7:279405/1‑142 (MQ=255)
                                                                                      tgtgGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAg                                                             <  8:279405/142‑1 (MQ=255)
                                                                                       gtgGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATtct                                                     >  2:312843/1‑149 (MQ=255)
                                                                                       gtgGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATtc                                                      >  6:18232/1‑148 (MQ=255)
                                                                                        tgGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATtct                                                     >  1:249500/1‑148 (MQ=255)
                                                                                            tatCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCAcc                                                 >  6:281392/1‑148 (MQ=255)
                                                                                             atCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCAtt                                                       <  8:121150/141‑1 (MQ=255)
                                                                                             atCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCAtt                                                       >  7:121150/1‑141 (MQ=255)
                                                                                                tcGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACTTCGCCCTCAGCGTCCCggg                                                                                   >  5:183568/1‑110 (MQ=255)
                                                                                                tcGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACTTCGCCCTCAGCGTCCCggg                                                                                   <  6:183568/110‑1 (MQ=255)
                                                                                                  gCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAg                                          >  8:148646/1‑149 (MQ=255)
                                                                                                   ccTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCAtt                                                       <  6:3210/135‑1 (MQ=255)
                                                                                                   ccTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCAtt                                                       >  5:3210/1‑135 (MQ=255)
                                                                                                    cTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGc                                                                <  6:340197/125‑1 (MQ=255)
                                                                                                    cTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGc                                                                >  5:340197/1‑125 (MQ=255)
                                                                                                       tGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGt                                      <  5:98315/148‑1 (MQ=255)
                                                                                                       tGCAGCCGAGCAACTGAGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGGGAGTTCATTCTCACCGCTCAAGGCGt                                      <  8:135939/148‑1 (MQ=255)
                                                                                                       caaaggcGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGt                                      <  6:88220/142‑1 (MQ=255)
                                                                                                        gCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTg                                     >  4:268709/1‑148 (MQ=255)
                                                                                                         cAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGa                                   <  6:36851/149‑1 (MQ=255)
                                                                                                              gAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCa                              <  1:312843/149‑1 (MQ=255)
                                                                                                                     tGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCCCTCAAGGCGTGGAAGGCAGCCTGgt                       >  2:347235/1‑149 (MQ=255)
                                                                                                                       cGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGgt                       <  6:167242/147‑1 (MQ=255)
                                                                                                                        ggTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTa                    >  8:315671/1‑149 (MQ=255)
                                                                                                                        ggTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTa                    <  5:281392/149‑1 (MQ=255)
                                                                                                                         gTTTTGAAGTAGCGGTCTGGAGCGCGGTACTCAAGCAGAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTGACCGCTCAAGGCGTGGAAGGCAGGCTGGTGTAt                   <  8:51045/149‑1 (MQ=255)
                                                                                                                          ttttGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATg                  >  4:94604/1‑149 (MQ=255)
                                                                                                                            cttgaGTAGCGCGCTCCAGCCCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGaa                                  >  7:294371/5‑131 (MQ=255)
                                                                                                                            cttgaGTAGCGCGCTCCAGCCCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGaa                                  <  8:294371/127‑1 (MQ=255)
                                                                                                                             ttgaGTAGCGCGCTCCAGCCCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAggg                                                                 >  3:289244/4‑99 (MQ=38)
                                                                                                                             ttgaGTAGCGCGCTCCAGCCCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAggg                                                                 <  4:289244/96‑1 (MQ=38)
                                                                                                                             tGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGcc                <  2:162825/148‑1 (MQ=255)
                                                                                                                               aaGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTg              <  1:340101/148‑1 (MQ=255)
                                                                                                                                 gTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTc           <  1:283543/149‑1 (MQ=255)
                                                                                                                                  tAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCCGCCAAGGGCGAGTTCATTCTCCCCGCTCAAGCCGTGGAAGGCAGCCTGGTGTAt                   >  7:195928/1‑140 (MQ=255)
                                                                                                                                      gggCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCAc      <  7:37893/149‑1 (MQ=255)
                                                                                                                                      gggCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCAc      <  1:275487/149‑1 (MQ=255)
                                                                                                                                      gggCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACAGCTGAAGAAAATCGACCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCAc      >  8:115495/1‑149 (MQ=255)
                                                                                                                                       ggCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGc                            >  3:238271/1‑126 (MQ=255)
                                                                                                                                       ggCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGc                            <  4:238271/126‑1 (MQ=255)
                                                                                                                                        gCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCACCg    <  6:3600/149‑1 (MQ=255)
                                                                                                                                        gCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCACCg    <  3:94604/149‑1 (MQ=255)
                                                                                                                                           ggAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCAc      >  4:232445/1‑144 (MQ=255)
                                                                                                                                           ggAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCAc      <  3:232445/144‑1 (MQ=255)
                                                                                                                                            gAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCACCGGt  <  7:200820/147‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
TCGGTCGTGGTGCTGGCGCTGGGTGGTGCCAGCTGGGCGCGCCTGGGTTCTGACGGCAGCTGGCAACCGCTGCTGGCAGAACGGGCTGTGGATATCTCGCCTTTGCAGCCGAGCAACTGCGGTTTTGAAGTAGCGGGCTGGAGCGCGCTACTCAAGGACAAGTTCGCGGGCGCACCGCTGAAGAACATCGCCCTCAGCGTCCCGGGCAGCGCACCGCGCAAGGGCGAGTTCATTCTCACCGCTCAAGGCGTGGAAGGCAGCCTGGTGTATGCCTGGTCGGCACCGGT  >  NC_002947/5425951‑5426237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑