Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,487,750 T→C 7.4% V360A (GTG→GCG) ‡ PP_4824 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,487,7500TC 7.4% 155.0 / 2.7 54V360A (GTG→GCG) ‡PP_4824two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base C (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

ACTGGTACAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAA  >  NC_002947/5487603‑5487896
                                                                                                                                                   |                                                                                                                                                  
aCTGGTACAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTg                                                                                                                                                   <  7:252277/149‑1 (MQ=255)
  tGGTACAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGt                                                                                                                                                 >  5:320193/1‑149 (MQ=255)
       cAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTg                                                                                                                                            >  3:351351/1‑149 (MQ=255)
       cAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCACGGTCAgg                                                                                                                                             >  4:156120/1‑147 (MQ=255)
       cAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCACGGTCAgg                                                                                                                                             <  7:293261/148‑2 (MQ=255)
       cAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCACGGTCAgg                                                                                                                                             <  3:156120/148‑2 (MQ=255)
        aaGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCCCCTCGACACCCTGGCTGCTGTGTCTCCTGCTGGGCCTGACCGTGGTCAGTgg                                                                                                                                           >  5:311006/1‑149 (MQ=255)
          gggTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGcc                                                                                                                                         >  8:212190/1‑149 (MQ=255)
             ttATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGc                                                                                                                                      <  6:168858/149‑1 (MQ=255)
                  cgcCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGctcct                                                                                                                                  <  3:204507/148‑1 (MQ=255)
                   gcCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTACCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGctcctc                                                                                                                                 >  3:158883/1‑148 (MQ=255)
                      cGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAAcc                                                                                                                             <  7:33793/149‑1 (MQ=255)
                          cGCCTGTTCAGCCTGGCGATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGTGCCTGACACGCACCTCGACACCCTTGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGc                                                                                                                         <  6:204949/149‑1 (MQ=255)
                          cGCCTGGTCAGCCTGGCCATGGGGGTGTTCAACCTCGGCGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGc                                                                                                                         <  6:311006/149‑1 (MQ=255)
                               gTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGt                                                                                                                      >  6:15843/1‑147 (MQ=255)
                               gTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGt                                                                                                                      >  2:259662/1‑147 (MQ=255)
                                ttCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCa                                                                                                                    <  5:15843/148‑1 (MQ=255)
                                ttCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCa                                                                                                                    <  1:259662/148‑1 (MQ=255)
                                   aGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGa                                                                                                                <  4:342114/149‑1 (MQ=255)
                                     ccTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAAc                                                                                                              <  3:16284/149‑1 (MQ=255)
                                            aTGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTgcg                                                                                                       >  4:271329/1‑149 (MQ=255)
                                              ggtggtGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTg                                                                                                                                            >  6:253994/1‑110 (MQ=255)
                                              ggtggtGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTg                                                                                                                                            <  5:253994/110‑1 (MQ=255)
                                                        aaCCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGc                                                                                             >  3:182632/1‑147 (MQ=255)
                                                               gAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGt                                                                                    >  6:111250/1‑149 (MQ=255)
                                                                aGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGtt                                                                                   <  6:135686/149‑1 (MQ=255)
                                                                    cTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAg                                                                               >  3:147950/1‑149 (MQ=255)
                                                                                ccAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGt                                                                     <  3:271329/147‑1 (MQ=255)
                                                                                ccAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTg                                                                    <  4:182632/148‑1 (MQ=255)
                                                                                      ctgctgGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGgc                                                             >  8:106106/1‑149 (MQ=255)
                                                                                                 gACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCACAGATg                                                  >  4:315813/1‑149 (MQ=255)
                                                                                                  acacGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGAt                                                   >  6:311035/1‑147 (MQ=255)
                                                                                                    acGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGAGCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCg                                                >  1:76580/1‑148 (MQ=255)
                                                                                                     ggCACCGCGACCCCCTGGCGGCTGTGTTTCCTGCTGGGCCGGACCGTGGTCTGTGGCCTGCTCCTCAACGTGGCGGTCAGTGACCGGCTGCGGCGCTTAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGa                                              <  4:351351/148‑1 (MQ=255)
                                                                                                      gCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGa                                              >  6:73707/1‑148 (MQ=255)
                                                                                                      gCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGa                                              >  1:99900/1‑148 (MQ=255)
                                                                                                          cTCGACACCCTGGCTGCTGTGTCTCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAAt                                           <  7:330701/147‑1 (MQ=255)
                                                                                                            cGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAAc                                       >  1:289353/1‑149 (MQ=255)
                                                                                                            cGACACCCTGGCTGCTGTGTATCCTGCTGGCCCTGACCGTGGGCAGTGGCCTGCTCCTCACCCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGATGAGCGGTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAAc                                       <  6:344906/149‑1 (MQ=255)
                                                                                                                  ccTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCa                                   >  5:175621/1‑147 (MQ=255)
                                                                                                                   cTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAg                                >  3:291664/1‑149 (MQ=255)
                                                                                                                     ggCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGcc                              <  4:158883/149‑1 (MQ=255)
                                                                                                                       ctgctgTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGa                            <  7:212190/149‑1 (MQ=255)
                                                                                                                        tgctgTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAg                           >  6:138866/1‑149 (MQ=255)
                                                                                                                          ctgTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGtt                         <  5:111250/149‑1 (MQ=255)
                                                                                                                            gtgtATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCgag                                                                                          <  2:283088/82‑1 (MQ=255)
                                                                                                                            gtgtATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCgag                                                                                          >  1:283088/1‑82 (MQ=255)
                                                                                                                                 tCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGc                   >  5:120143/1‑148 (MQ=255)
                                                                                                                                  ccTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATCCGGAAATCAACGCCAAAGCCGAGTTTCTGGcc                  >  5:18518/1‑148 (MQ=255)
                                                                                                                                     caggccacTGACCACGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGcc                  >  4:33001/8‑145 (MQ=255)
                                                                                                                                         ggCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGc                               >  1:368183/1‑128 (MQ=255)
                                                                                                                                         ggCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCa           <  6:175621/148‑1 (MQ=255)
                                                                                                                                                ccGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGaa   <  4:147950/149‑1 (MQ=255)
                                                                                                                                                 cGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGaaa  >  1:220189/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACTGGTACAAGGGTTATGCGCCCGGACGCCTGTTCAGCCTGGCCATGGTGGTGTTCAACCTCGGAGGCCTGGTACTGCTGCCAGCCCTGCTGGGCCTGACACGCACCTCGACACCCTGGCTGCTGTGTATCCTGCTGGGCCTGACCGTGGTCAGTGGCCTGCTCCTCAACCTGGCGGTCAGTGAACGGCTGCGGCGCATAAGCGAGGAGCGTTTCAGCGCCAGCCGTGCACTGGCCGCCAGCGATGCGGAAATCAACGCCAAAGCCGAGTTTCTGGCCAAGATCAGCCACGAAA  >  NC_002947/5487603‑5487896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑