Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,491,442 C→A 5.4% W8L (TGG→TTG)  PP_5715 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,491,4420CA 5.4% 241.6 / 3.6 74W8L (TGG→TTG) PP_5715hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/36);  new base A (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

GCAGATAGTCCTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGT  >  NC_002947/5491296‑5491589
                                                                                                                                                  |                                                                                                                                                    
gCAGATAGTCCTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACca                                                                                                                                                     >  7:312964/1‑148 (MQ=255)
   gATAGTCCTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACaaa                                                                                                                                                 <  6:331921/149‑1 (MQ=255)
    aTAGTCCTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAg                                                                                                                                                >  3:202406/1‑149 (MQ=255)
        tCCTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAgtgt                                                                                                                                             >  8:44143/1‑148 (MQ=255)
         ccTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTaa                                                                                                                                           >  5:314901/1‑149 (MQ=255)
          cTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACAACAAAGTGTAAt                                                                                                                                          >  6:93552/1‑149 (MQ=255)
          cTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACAACAAAGTGTAAt                                                                                                                                          >  6:88394/1‑149 (MQ=255)
             gtgCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAAtt                                                                                                                                         <  2:267689/147‑1 (MQ=255)
               gCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGc                                                                                                                                      <  6:46981/148‑1 (MQ=255)
                  aaTCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGGCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGc                                                                                                                                  <  2:159622/149‑1 (MQ=255)
                      aGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCAt                                                                                                                                >  1:218863/1‑147 (MQ=255)
                      aGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCa                                                                                                                              <  3:289314/149‑1 (MQ=255)
                      aGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACAACAAAGTGTAATTGGCTTGCATc                                                                                                                               <  5:93552/148‑1 (MQ=255)
                      aGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACAACAAAGTGTAATTGGCTTGCATc                                                                                                                               <  5:88394/148‑1 (MQ=255)
                               aaTGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑Gccc                                                                                                                     <  6:314901/148‑1 (MQ=255)
                                 tGGCGTGCCCCTTGTTAGCCGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTcc                                                                                                                  <  4:202406/149‑1 (MQ=255)
                                         cccTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGt                                                                                                          >  7:169987/1‑149 (MQ=255)
                                         cccTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCAATGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCtt                                                                                                                                    <  6:22428/124‑1 (MQ=255)
                                         cccTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATACAACAGTGCTTCTGCGCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCtt                                                                                                                                    >  5:22428/1‑124 (MQ=255)
                                          ccTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTc                                                                                                         <  5:267823/149‑1 (MQ=255)
                                          ccTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTc                                                                                                         >  4:198502/1‑149 (MQ=255)
                                               ttAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGc                                                                                                    >  5:177095/1‑149 (MQ=255)
                                                  gCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCgt                                                                                                 >  5:51604/1‑149 (MQ=255)
                                                    ggTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCgtgt                                                                                               <  2:218863/149‑1 (MQ=255)
                                                     gTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTc                                                                                              <  7:77105/149‑1 (MQ=255)
                                                     gTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTAATGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAgg                                                                                                               >  1:230487/1‑132 (MQ=255)
                                                     gTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTAATGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAgg                                                                                                               <  2:230487/132‑1 (MQ=255)
                                                        aGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGAc                                                                                           <  3:198502/149‑1 (MQ=255)
                                                          gTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCTGTCGACACGGCGTCGAcgg                                                                                         >  2:61319/1‑147 (MQ=255)
                                                           tAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCTATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                         <  8:169987/148‑1 (MQ=255)
                                                               cTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATCGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcct                                                                                     <  1:61319/149‑2 (MQ=255)
                                                                  ccAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCATTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAAc                                                                                   <  7:260131/147‑1 (MQ=255)
                                                                  ccAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACtt                                                                                 >  3:233253/1‑149 (MQ=255)
                                                                     gCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCAt                                                                              <  6:177095/149‑1 (MQ=255)
                                                                      cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                             <  6:51604/149‑1 (MQ=255)
                                                                         aaCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGt                                                                           >  7:42868/1‑148 (MQ=255)
                                                                              ccAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                             <  6:84133/141‑1 (MQ=255)
                                                                              ccAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                             >  5:84133/1‑141 (MQ=255)
                                                                              ccAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGt                                                                     >  2:159270/1‑149 (MQ=255)
                                                                               cAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCGCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGtt                                                                    >  5:320126/1‑149 (MQ=255)
                                                                                aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCa                                                                         <  6:357968/143‑1 (MQ=255)
                                                                                aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCa                                                                         >  5:357968/1‑143 (MQ=255)
                                                                                  cAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCa                                                                 <  8:42868/149‑1 (MQ=255)
                                                                                       cTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGc                                                             <  7:44143/148‑1 (MQ=255)
                                                                                        ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCagag                                                                      <  6:271657/138‑1 (MQ=255)
                                                                                        ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCagag                                                                      >  5:271657/1‑138 (MQ=255)
                                                                                            gCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagcag                                                       >  4:42943/1‑149 (MQ=255)
                                                                                            cgagcagAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagcag                                                       <  7:178089/143‑1 (MQ=255)
                                                                                             cTCGTGAAGCACTGACATTGTCGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCAt                                                                              >  5:358113/1‑125 (MQ=255)
                                                                                             cTCGTGAAGCACTGACATTGTCGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCAt                                                                              <  6:358113/125‑1 (MQ=255)
                                                                                                gTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGAc                                                    <  8:312964/148‑1 (MQ=255)
                                                                                                     gCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTGAGAGTTCCAGTGCCAGCAGGACCTGCAg                                              <  1:205668/149‑1 (MQ=255)
                                                                                                     gCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCATGACCTGCAg                                              >  1:312424/1‑149 (MQ=255)
                                                                                                     gCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                              >  4:198614/1‑149 (MQ=255)
                                                                                                         tGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGc                                                >  8:17541/1‑143 (MQ=255)
                                                                                                         tGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGc                                                <  7:17541/143‑1 (MQ=255)
                                                                                                           aCATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCAtt                                        >  5:208754/1‑149 (MQ=255)
                                                                                                             aTTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGTCATTgt                                      >  1:369667/1‑149 (MQ=255)
                                                                                                               tgttgtCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtcg                                    >  6:321159/1‑149 (MQ=255)
                                                                                                               tgttgtCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtcg                                    >  2:328196/1‑149 (MQ=255)
                                                                                                                gttgtCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtcg                                    <  4:39559/148‑1 (MQ=255)
                                                                                                                gttgtCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtcg                                    >  3:39559/1‑148 (MQ=255)
                                                                                                                 ttgtCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtcgtc                                  <  3:198614/149‑1 (MQ=255)
                                                                                                                    tCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAg                               >  3:356574/1‑149 (MQ=255)
                                                                                                                      gATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                             >  3:180500/1‑149 (MQ=255)
                                                                                                                       aTCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACGTGCAGGGCATTGGCGTGAAGttt                            >  8:178948/1‑149 (MQ=255)
                                                                                                                          aaTACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGt                         <  4:356574/149‑1 (MQ=255)
                                                                                                                               cAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCTGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTa                    <  1:328196/149‑1 (MQ=255)
                                                                                                                               cAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTc                      <  8:7395/147‑1 (MQ=255)
                                                                                                                               cAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTc                      >  7:7395/1‑147 (MQ=255)
                                                                                                                                      ttGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagc                                                         <  6:326776/105‑1 (MQ=255)
                                                                                                                                      ttGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagc                                                         >  5:326776/1‑105 (MQ=255)
                                                                                                                                           gTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa        <  5:321159/149‑1 (MQ=255)
                                                                                                                                                aCCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGt                         <  5:324076/127‑1 (MQ=255)
                                                                                                                                                aCCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCATCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGt                         >  6:324076/1‑127 (MQ=255)
                                                                                                                                                 ccACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt  >  5:205819/1‑149 (MQ=255)
                                                                                                                                                 ccACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt  >  5:200720/1‑149 (MQ=255)
                                                                                                                                                 ccACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt  <  4:233253/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCAGATAGTCCTTGTGCCAATCAGCTTCCTGAATGGCGTGCCCCTTGTTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTAT‑GCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGT  >  NC_002947/5491296‑5491589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑