Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 373,069 T→A 7.3% I104N (ATC→AAC)  dgcB → dimethylglycine dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947373,0690TA 7.3% 158.3 / 2.8 55I104N (ATC→AAC) dgcBdimethylglycine dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base T (29/22);  new base A (2/2);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

ACGTGGTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCGGTCGCCGGAATCCT  >  NC_002947/372922‑373216
                                                                                                                                                   |                                                                                                                                                   
acGTGGTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATc                                                                                                                                                    <  2:214754/149‑1 (MQ=255)
  gTGGTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCAt                                                                                                                                                  >  5:14413/1‑149 (MQ=255)
    ggTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGt                                                                                                                                                >  3:88492/1‑149 (MQ=255)
    ggTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGAACATga                                                                                                                                                >  3:292500/1‑148 (MQ=255)
     gTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGtt                                                                                                                                               <  4:127106/149‑1 (MQ=255)
     gTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGtt                                                                                                                                               <  4:121759/149‑1 (MQ=255)
            gcgACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGAACATGATCACCgtg                                                                                                                                        <  4:292500/149‑3 (MQ=255)
                   aTACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCAc                                                                                                                                            <  4:192876/138‑1 (MQ=255)
                   aTACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCAc                                                                                                                                            >  3:192876/1‑138 (MQ=255)
                       aTGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGc                                                                                                                                      <  2:79690/140‑1 (MQ=255)
                       aTGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGc                                                                                                                                      >  1:79690/1‑140 (MQ=255)
                       aTGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGt                                                                                                                             <  6:14413/149‑1 (MQ=255)
                             aaGACCCAAGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCa                                                                                                                        >  6:156421/1‑148 (MQ=255)
                                          ccACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAAc                                                                                                          >  4:332130/1‑149 (MQ=255)
                                           cACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAAcc                                                                                                         >  4:174760/1‑149 (MQ=255)
                                                   gcggcTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCctct                                                                                                 <  3:174760/149‑1 (MQ=255)
                                                            tgctgtctgctgtcCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTc                                                                                         >  6:379/1‑148 (MQ=255)
                                                               cgtctgctgtcCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAg                                                                                     >  6:266878/2‑149 (MQ=255)
                                                                 tctgctgtcCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAggg                                                                                   >  6:142793/1‑149 (MQ=255)
                                                                          cTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGAt                                                                          <  5:94914/149‑1 (MQ=255)
                                                                           tGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATg                                                                         >  4:52452/1‑149 (MQ=255)
                                                                            ggCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATgc                                                                        >  3:45577/1‑149 (MQ=255)
                                                                             gCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATgcg                                                                       >  1:94492/1‑149 (MQ=255)
                                                                                      gTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGaa                                                              <  5:266878/149‑1 (MQ=255)
                                                                                         cATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATGATTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAagag                                                           >  7:235516/1‑149 (MQ=255)
                                                                                         cATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAagag                                                           >  3:30565/1‑149 (MQ=255)
                                                                                         cATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAagag                                                           >  7:163913/1‑149 (MQ=255)
                                                                                              ttttGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCaa                                                                                      >  2:181696/1‑117 (MQ=255)
                                                                                              ttttGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCaa                                                                                      <  1:181696/117‑1 (MQ=255)
                                                                                                ttGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCt                                                     <  2:94492/148‑1 (MQ=255)
                                                                                                 tGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTg                                                    <  5:142793/148‑1 (MQ=255)
                                                                                                   gCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGc                                                  <  4:88492/148‑1 (MQ=255)
                                                                                                      tGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTc                                              >  1:18431/1‑149 (MQ=255)
                                                                                                           agCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGt                                         >  8:146637/1‑149 (MQ=255)
                                                                                                              aaGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAg                                      >  4:353692/1‑149 (MQ=255)
                                                                                                                gATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTg                                                       <  8:203570/130‑1 (MQ=255)
                                                                                                                gATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTg                                                       >  7:203570/1‑130 (MQ=255)
                                                                                                                   ccTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGcttct                                 >  7:46449/1‑149 (MQ=255)
                                                                                                                   ccTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGcttct                                 >  2:172897/1‑149 (MQ=255)
                                                                                                                     tCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGcttctt                                >  2:199910/1‑148 (MQ=255)
                                                                                                                        gCTACGCACTGCTGATCGCCCCGGTGATCATGTTCACCGGTCCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCAtt                            <  2:119373/149‑1 (MQ=255)
                                                                                                                        ccaccccACTGCCGACCGCCACGGCGATCATGTTCACCGGTGCCCTCTTAGTCTTCAACCGCCGCCTCAACCCGCCCTCTCGCCTGTCCACGGGCCCGTGGATGCGCCGGCCGAAGAGTTTGCTGCCGTTCGCCGTGAGCTTCTTCAtt                            <  8:235516/143‑1 (MQ=255)
                                                                                                                             gCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCAc                       >  1:148789/1‑149 (MQ=255)
                                                                                                                             gCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCAc                       >  3:295820/1‑149 (MQ=255)
                                                                                                                               aCTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCAccc                     >  1:88578/1‑149 (MQ=255)
                                                                                                                                 tgctgATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTg                   >  6:121130/1‑149 (MQ=255)
                                                                                                                                   ctgATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGcc                 <  4:30565/149‑1 (MQ=255)
                                                                                                                                   ctgATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGc                  <  5:303834/148‑1 (MQ=255)
                                                                                                                                      aTCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCg                <  8:46449/147‑1 (MQ=255)
                                                                                                                                          ccACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCGGTCGcc          >  2:284283/1‑149 (MQ=255)
                                                                                                                                           accGGTGAACATGATCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCAccc                     <  6:78767/135‑1 (MQ=255)
                                                                                                                                           accGGTGAACATGATCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCAccc                     >  5:78767/3‑137 (MQ=255)
                                                                                                                                              ggTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCACGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCGGTCGCCGGaa      <  5:156421/149‑1 (MQ=255)
                                                                                                                                               gTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCGGTCGCCGGAAt     <  4:45577/149‑1 (MQ=255)
                                                                                                                                                  aTCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAGGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCGGTCGCCGGAATCCt  <  5:379/149‑1 (MQ=255)
                                                                                                                                                   tCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCg                >  6:317051/1‑134 (MQ=255)
                                                                                                                                                   tCATGTTCACCGGTGCCATCTTTGTCCTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCg                <  5:317051/134‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACGTGGTTGAGCGCGACAAATACATGTCCAAGACCCACGTGGCCACTGCGGGCGGCTTTGTGCTGTCTGCTGTCCTGGCCATTCTGGTGCATGGTTTTGGCCTGCAGAGCAAGATCCTCGGCTACGCACTGCTGATCGCCACGGTGATCATGTTCACCGGTGCCATCTTTGTCTTCAAACGCCGCCTCAACCCGCCCTCTCGCCTGTCCAAGGGCCCGTGGATGCGCCTGCCGAAGAGTTTGCTGGCGTTCGCCGTGAGCTTCTTCATTGCCACCCTGCCGGTCGCCGGAATCCT  >  NC_002947/372922‑373216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑