Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,517,787 A→G 8.0% I265T (ATC→ACC)  PP_4850 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,517,7870AG 8.0% 176.9 / 3.5 62I265T (ATC→ACC) PP_4850membrane protein
Reads supporting (aligned to +/- strand):  ref base A (30/27);  new base G (3/2);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGATCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGAT  >  NC_002947/5517642‑5517935
                                                                                                                                                 |                                                                                                                                                    
aCTGATCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCa                                                                                                                                                   >  2:360122/1‑149 (MQ=255)
    aTCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGATGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCAcc                                                                                                                                                 <  6:311724/147‑1 (MQ=255)
    aTCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCgg                                                                                                                                               >  7:22791/1‑149 (MQ=255)
    aTCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCg                                                                                                                                                <  3:261686/148‑1 (MQ=255)
         cccacccaCGATCCGCGCGTTGAGGAAGTAGTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGTACATCAGGTTGCCGATCACCGgca                                                                                                                                             <  7:291317/146‑1 (MQ=255)
           cacccacGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCCCCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGCTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAg                                                                                                                                        >  4:39112/1‑149 (MQ=255)
              ccacGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGcc                                                                                                                                      >  8:159602/1‑148 (MQ=255)
              ccacGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATCGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGcc                                                                                                                                      >  5:186451/1‑148 (MQ=255)
                acGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTTGACATCAGGTTGCCGATCACCGGCAGCAAGCCcag                                                                                                                                   <  3:199791/149‑1 (MQ=255)
                acGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcag                                                                                                                                   >  4:199791/1‑149 (MQ=255)
                  gATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcagca                                                                                                                                 <  1:360122/149‑1 (MQ=255)
                   aTCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcagcag                                                                                                                                <  4:168868/149‑1 (MQ=255)
                    tCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCATCGGCAGCAAGCCcagcag                                                                                                                                >  8:164390/1‑148 (MQ=255)
                       gcgcgTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAg                                                                                                                            <  8:22791/149‑1 (MQ=255)
                           gTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGt                                                                                                                          >  6:50531/1‑147 (MQ=255)
                                 gAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGa                                                                                                                  >  3:97821/1‑149 (MQ=255)
                                       cTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGc                                                                                                             <  6:308800/148‑1 (MQ=255)
                                                gtgGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGt                                                                                                     <  7:159602/147‑1 (MQ=255)
                                                gtgGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTc                                                                                                    <  4:77562/148‑1 (MQ=255)
                                                  ggATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcag                                                                                                                                   >  6:113928/1‑115 (MQ=255)
                                                       acgccCAGCTAACCCAGCGCCGCGGCCGCCACCCCGATCGACAGCGACAGGCCGACGATAGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAg                                                                                            <  6:257136/149‑1 (MQ=255)
                                                          acCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGgtg                                                                                         <  2:115843/149‑1 (MQ=255)
                                                           ccAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGgtg                                                                                         >  8:11576/1‑148 (MQ=255)
                                                               gTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGt                                                                                    <  5:50531/149‑1 (MQ=255)
                                                                   cccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTc                                                                                   >  6:320551/1‑146 (MQ=255)
                                                                   cccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTc                                                                                   <  5:320551/146‑1 (MQ=255)
                                                                   cccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                >  6:110793/1‑149 (MQ=255)
                                                                    ccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                >  4:199311/1‑148 (MQ=255)
                                                                    ccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                >  5:236304/1‑148 (MQ=255)
                                                                    ccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                >  1:308836/1‑148 (MQ=255)
                                                                       gcgcCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGt                                                                            <  1:232361/149‑1 (MQ=255)
                                                                          ccgcTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTGAGCGGCAGGTGTACGTCGAACAGTgg                                                                          >  7:255197/1‑148 (MQ=255)
                                                                                  gccACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATcaccac                                                                 >  3:148626/1‑149 (MQ=255)
                                                                                   ccACCCAGATCAACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACg                                                                >  3:332370/1‑149 (MQ=255)
                                                                                       ccAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATcaccac                                                                 >  5:262926/1‑144 (MQ=255)
                                                                                       ccAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATcaccac                                                                 <  6:262926/144‑1 (MQ=255)
                                                                                                   cgacagGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCgg                                                >  2:264962/1‑149 (MQ=255)
                                                                                                    gacagGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGt                                               <  4:332370/149‑1 (MQ=255)
                                                                                                    gacagGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGt                                               >  2:280412/1‑149 (MQ=255)
                                                                                                           cgacgaTGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATc                                        <  7:11576/149‑1 (MQ=255)
                                                                                                                aTGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCATCGGCAGCAAGCCCAGCAGGAAGGGCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGtt                                   <  7:164390/149‑1 (MQ=255)
                                                                                                                   gTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGa                                 <  1:301597/148‑1 (MQ=255)
                                                                                                                         aaGGTGTTGGACATCAGGTTGCCGATCAGCGGGAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTGTTGGTCAGCGGCAGGTGTACGTCGAAGAGTGGCAACACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCga                          <  3:39112/149‑1 (MQ=255)
                                                                                                                         aaGGTGTTGGACATCAGGTTGCCGATCACCGGCAGTAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGagcagc                            >  2:184515/1‑147 (MQ=255)
                                                                                                                          aGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGagcagc                            >  6:267968/1‑146 (MQ=255)
                                                                                                                                 ggACATCAGGTTGCCGGTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATc                      >  1:103764/1‑145 (MQ=255)
                                                                                                                                 ggACATCAGGTTGCCGGTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATc                      <  2:103764/145‑1 (MQ=255)
                                                                                                                                 ggACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTg                   <  5:110793/148‑1 (MQ=255)
                                                                                                                                     aTCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCga                          >  8:26240/1‑137 (MQ=255)
                                                                                                                                     aTCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCga                          <  7:26240/137‑1 (MQ=255)
                                                                                                                                      gcttgctGCCGGTGATCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGAACGTGTTGAGCCGCGAGCTCTTGATTGgc             >  8:159496/7‑149 (MQ=255)
                                                                                                                                        ttgctGCCGGTGATCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGa                                 >  8:321448/5‑127 (MQ=255)
                                                                                                                                        ttgctGCCGGTGATCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGa                                 <  7:321448/123‑1 (MQ=255)
                                                                                                                                        aGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTgcgc           <  6:236304/149‑1 (MQ=255)
                                                                                                                                        aGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTgcgc           <  3:199311/149‑1 (MQ=255)
                                                                                                                                           ttGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCt                     >  1:217581/1‑136 (MQ=255)
                                                                                                                                           ttGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCt                     <  2:217581/136‑1 (MQ=255)
                                                                                                                                             gCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAaca      >  3:154264/1‑149 (MQ=255)
                                                                                                                                               cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTGAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAAGACg    >  8:294554/1‑149 (MQ=255)
                                                                                                                                               cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGtt                                   >  7:214747/1‑118 (MQ=255)
                                                                                                                                               cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGtt                                   <  8:214747/118‑1 (MQ=255)
                                                                                                                                               cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAacac     >  8:75478/1‑148 (MQ=255)
                                                                                                                                                 aTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGAt  <  6:186451/149‑1 (MQ=255)
                                                                                                                                                 aTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGAt  <  5:267968/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACTGATCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGAT  >  NC_002947/5517642‑5517935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑