Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,517,791 C→T 8.0% V264M (GTG→ATG)  PP_4850 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,517,7910CT 8.0% 175.7 / 3.2 63V264M (GTG→ATG) PP_4850membrane protein
Reads supporting (aligned to +/- strand):  ref base C (30/27);  new base T (3/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGATGTTG  >  NC_002947/5517646‑5517939
                                                                                                                                                 |                                                                                                                                                    
aTCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGATGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCAcc                                                                                                                                                     <  6:311724/147‑1 (MQ=255)
aTCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCgg                                                                                                                                                   >  7:22791/1‑149 (MQ=255)
aTCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCg                                                                                                                                                    <  3:261686/148‑1 (MQ=255)
     cccacccaCGATCCGCGCGTTGAGGAAGTAGTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGTACATCAGGTTGCCGATCACCGgca                                                                                                                                                 <  7:291317/146‑1 (MQ=255)
       cacccacGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCCCCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGCTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAg                                                                                                                                            >  4:39112/1‑149 (MQ=255)
          ccacGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGcc                                                                                                                                          >  8:159602/1‑148 (MQ=255)
          ccacGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATCGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGcc                                                                                                                                          >  5:186451/1‑148 (MQ=255)
            acGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTTGACATCAGGTTGCCGATCACCGGCAGCAAGCCcag                                                                                                                                       <  3:199791/149‑1 (MQ=255)
            acGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcag                                                                                                                                       >  4:199791/1‑149 (MQ=255)
              gATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcagca                                                                                                                                     <  1:360122/149‑1 (MQ=255)
               aTCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcagcag                                                                                                                                    <  4:168868/149‑1 (MQ=255)
                tCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCATCGGCAGCAAGCCcagcag                                                                                                                                    >  8:164390/1‑148 (MQ=255)
                   gcgcgTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAg                                                                                                                                <  8:22791/149‑1 (MQ=255)
                       gTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGt                                                                                                                              >  6:50531/1‑147 (MQ=255)
                             gAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGa                                                                                                                      >  3:97821/1‑149 (MQ=255)
                                   cTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGc                                                                                                                 <  6:308800/148‑1 (MQ=255)
                                            gtgGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGt                                                                                                         <  7:159602/147‑1 (MQ=255)
                                            gtgGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTc                                                                                                        <  4:77562/148‑1 (MQ=255)
                                              ggATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCcag                                                                                                                                       >  6:113928/1‑115 (MQ=255)
                                                   acgccCAGCTAACCCAGCGCCGCGGCCGCCACCCCGATCGACAGCGACAGGCCGACGATAGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAg                                                                                                <  6:257136/149‑1 (MQ=255)
                                                      acCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGgtg                                                                                             <  2:115843/149‑1 (MQ=255)
                                                       ccAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGgtg                                                                                             >  8:11576/1‑148 (MQ=255)
                                                           gTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGt                                                                                        <  5:50531/149‑1 (MQ=255)
                                                               cccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTc                                                                                       <  5:320551/146‑1 (MQ=255)
                                                               cccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTc                                                                                       >  6:320551/1‑146 (MQ=255)
                                                               cccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                    >  6:110793/1‑149 (MQ=255)
                                                                ccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                    >  4:199311/1‑148 (MQ=255)
                                                                ccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                    >  1:308836/1‑148 (MQ=255)
                                                                ccAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGaa                                                                                    >  5:236304/1‑148 (MQ=255)
                                                                   gcgcCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGt                                                                                <  1:232361/149‑1 (MQ=255)
                                                                      ccgcTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTGAGCGGCAGGTGTACGTCGAACAGTgg                                                                              >  7:255197/1‑148 (MQ=255)
                                                                              gccACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATcaccac                                                                     >  3:148626/1‑149 (MQ=255)
                                                                               ccACCCAGATCAACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACg                                                                    >  3:332370/1‑149 (MQ=255)
                                                                                   ccAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATcaccac                                                                     >  5:262926/1‑144 (MQ=255)
                                                                                   ccAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATcaccac                                                                     <  6:262926/144‑1 (MQ=255)
                                                                                               cgacagGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCgg                                                    >  2:264962/1‑149 (MQ=255)
                                                                                                gacagGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGt                                                   <  4:332370/149‑1 (MQ=255)
                                                                                                gacagGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGt                                                   >  2:280412/1‑149 (MQ=255)
                                                                                                       cgacgaTGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATc                                            <  7:11576/149‑1 (MQ=255)
                                                                                                            aTGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCATCGGCAGCAAGCCCAGCAGGAAGGGCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGtt                                       <  7:164390/149‑1 (MQ=255)
                                                                                                               gTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGa                                     <  1:301597/148‑1 (MQ=255)
                                                                                                                     aaGGTGTTGGACATCAGGTTGCCGATCAGCGGGAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTGTTGGTCAGCGGCAGGTGTACGTCGAAGAGTGGCAACACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCga                              <  3:39112/149‑1 (MQ=255)
                                                                                                                     aaGGTGTTGGACATCAGGTTGCCGATCACCGGCAGTAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGagcagc                                >  2:184515/1‑147 (MQ=255)
                                                                                                                      aGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGagcagc                                >  6:267968/1‑146 (MQ=255)
                                                                                                                             ggACATCAGGTTGCCGGTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATc                          >  1:103764/1‑145 (MQ=255)
                                                                                                                             ggACATCAGGTTGCCGGTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATc                          <  2:103764/145‑1 (MQ=255)
                                                                                                                             ggACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTg                       <  5:110793/148‑1 (MQ=255)
                                                                                                                                 aTCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCga                              >  8:26240/1‑137 (MQ=255)
                                                                                                                                 aTCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCga                              <  7:26240/137‑1 (MQ=255)
                                                                                                                                  gcttgctGCCGGTGATCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGAACGTGTTGAGCCGCGAGCTCTTGATTGgc                 >  8:159496/7‑149 (MQ=255)
                                                                                                                                    ttgctGCCGGTGATCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGa                                     >  8:321448/5‑127 (MQ=255)
                                                                                                                                    ttgctGCCGGTGATCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGa                                     <  7:321448/123‑1 (MQ=255)
                                                                                                                                    aGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTgcgc               <  6:236304/149‑1 (MQ=255)
                                                                                                                                    aGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTgcgc               <  3:199311/149‑1 (MQ=255)
                                                                                                                                       ttGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCt                         <  2:217581/136‑1 (MQ=255)
                                                                                                                                       ttGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCt                         >  1:217581/1‑136 (MQ=255)
                                                                                                                                         gCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAaca          >  3:154264/1‑149 (MQ=255)
                                                                                                                                           cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTGAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAAGACg        >  8:294554/1‑149 (MQ=255)
                                                                                                                                           cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGtt                                       >  7:214747/1‑118 (MQ=255)
                                                                                                                                           cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGtt                                       <  8:214747/118‑1 (MQ=255)
                                                                                                                                           cGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAacac         >  8:75478/1‑148 (MQ=255)
                                                                                                                                             aTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGAt      <  6:186451/149‑1 (MQ=255)
                                                                                                                                             aTCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGAt      <  5:267968/149‑1 (MQ=255)
                                                                                                                                              tCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCCTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGATg     >  1:179794/1‑149 (MQ=255)
                                                                                                                                                 ccGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGATGTTg  >  7:4872/1‑149 (MQ=255)
                                                                                                                                                 ccGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGATGTTg  <  4:148626/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATCTGCCCACCCACGATCCGCGCGTTGAGGAAGTACTCGATCTTGTGGATGACGACCAGGTAACCCAGCGCCGCTGCCGCCACCCAGATCGACAGCGACAGGCCGACGATGGTGATCAAGGTGTTGGACATCAGGTTGCCGATCACCGGCAGCAAGCCCAGCAGGAAGGTCAGCACGATCAGCGTCTTGGTCAGCGGCAGGTGTACGTCGAACAGTGGCATCACCACGGCCAGGAAAATGCCGGTGAAGATCGTGTTGAGCAGCGAGATCTTGATTTGCGCGAACACGATGTTG  >  NC_002947/5517646‑5517939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑