Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,549,605 T→A 12.0% intergenic (+18/‑154) PP_t47 → / → PP_4881 tRNA‑Leu/iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,549,6050TA12.0% 135.2 / 5.9 55intergenic (+18/‑154)PP_t47/PP_4881tRNA‑Leu/iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base A (3/4);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGAAAGCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAGGAA  >  NC_002947/5549457‑5549750
                                                                                                                                                    |                                                                                                                                                 
tgAAAGCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGCCGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCt                                                                                                                                                   >  5:120552/1‑149 (MQ=255)
  aaaGCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTg                                                                                                                                                 >  2:235892/1‑149 (MQ=255)
    aGCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGc                                                                                                                                                >  2:52281/1‑148 (MQ=255)
        aaCGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTAACAGTGACCGAAAGGTCGTGGAACTTCGAGTCATCTCCTGGGCACCATACAAAAACCCACTAGCATGCTAgag                                                                                                                                           >  5:155438/1‑147 (MQ=21)
         aCGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCTGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGTgg                                                                                                                                          <  3:135328/149‑1 (MQ=255)
         aCGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGTgg                                                                                                                                          <  5:335877/149‑1 (MQ=255)
             tAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGttt                                                                                                                                      >  8:145291/1‑149 (MQ=255)
               gTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGTGGGttttt                                                                                                                                    <  5:364955/149‑1 (MQ=255)
                   ccTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCtt                                                                                                                                <  7:145291/149‑1 (MQ=255)
                        tGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTg                                                                                                                           >  5:26669/1‑149 (MQ=255)
                            gatgatGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGAt                                                                                                                       <  1:52281/149‑1 (MQ=255)
                                atgTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGTTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTAt                                                                                                                   <  1:235892/149‑1 (MQ=255)
                                   tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTAtgt                                                                                                                 <  4:181683/148‑1 (MQ=255)
                                    aaaGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTAtgtgg                                                                                                               >  8:205462/1‑148 (MQ=255)
                                         tttGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGccccat                                                                                                          >  7:324875/1‑147 (MQ=255)
                                             cccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTcccc                                                                                                      >  8:7683/1‑149 (MQ=255)
                                             cccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTcccc                                                                                                      >  2:271312/1‑149 (MQ=255)
                                               cAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTcccccc                                                                                                    >  5:156015/1‑149 (MQ=255)
                                                aGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCTTccccccc                                                                                                   <  8:324875/149‑1 (MQ=255)
                                                       ggAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTg                                                                                                                               <  6:285154/114‑1 (MQ=255)
                                                       ggAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTg                                                                                                                               >  5:285154/1‑114 (MQ=255)
                                                       ggAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTCGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCAc                                                                                             >  4:65705/1‑148 (MQ=255)
                                                              gTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGCGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTGTTTTCTTGCCTGCGAGTTATGTGCCGCCTGCCCCCCGTGCCCCCTggggt                                                                                     >  2:243768/1‑146 (MQ=21)
                                                                 gACGCGCTGGTGTCAGGTATCAGTGACCGCAAGGTCGTGGTAGTTCTCGTCTTCTGCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGAc                                                                                  <  6:26669/149‑1 (MQ=38)
                                                                    gcgcTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCGTGCACCCTGGGGGGACCAt                                                                               >  2:233050/1‑149 (MQ=37)
                                                                          gTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAAt                                                                           <  8:205282/147‑1 (MQ=255)
                                                                           tttCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTtc                                                                        <  1:271312/149‑1 (MQ=255)
                                                                           tttCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTtc                                                                        <  6:156015/149‑1 (MQ=255)
                                                                               aGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGt                                                                    <  6:32810/149‑1 (MQ=255)
                                                                                 gTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTca                                                                  <  7:7683/149‑1 (MQ=255)
                                                                                    taagggACCGCAAGGTCGTTGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGGTAGTGGGTTTTTTCTTGCATGCGATTTATTTGCCCCCTCCCCCCCTTGCTACATGGTCTGACCATGAATTTCTCGTcaca                                                                <  6:155438/143‑1 (MQ=38)
                                                                                    tCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGAc                                                                                  >  2:324249/1‑130 (MQ=255)
                                                                                    tCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGAc                                                                                  <  1:324249/130‑1 (MQ=255)
                                                                                      aGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACgg                                                             >  4:364317/1‑149 (MQ=255)
                                                                                        tGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCCATTTATGTGCCCCCAGCCCCGCTGGCACCATGGGCTGGCCATGGATATCTCGTCACAAGGc                                                            >  1:365036/1‑148 (MQ=37)
                                                                                          aCCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGGCGATGCATTTCTCGTCACACGGGCAc                                                         >  4:359603/1‑149 (MQ=255)
                                                                                           ccGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCt                                                                                                                                               >  1:318937/1‑62 (MQ=255)
                                                                                           ccGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCt                                                                                                                                               <  2:318937/62‑1 (MQ=255)
                                                                                              cAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTga                                                     <  1:233050/149‑1 (MQ=255)
                                                                                                     gTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGACCACTCGCCCCCGTGCACCATGGTCGTACAATGAATTTGTAGGCACACGGCCACGTGGGaaaaa                                               >  2:244146/1‑146 (MQ=255)
                                                                                                     gTGGAAGTTCGAGTCTTATCCTGGGCACCATACTAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACa                                               <  1:243768/148‑1 (MQ=255)
                                                                                                         gttctggAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGGAACAATTTc                                          <  3:192069/143‑1 (MQ=255)
                                                                                                         aaGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTc                                          <  6:140288/149‑1 (MQ=255)
                                                                                                             tCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGtt                                       <  7:205462/148‑1 (MQ=255)
                                                                                                               gAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCGGCTCCCCCCCTGGCCACGTGGTCGCCCCAGGAATTAATCGGAGCCCGGTCACTAGAGGAGCCATTTCGtga                                      >  4:192069/1‑145 (MQ=255)
                                                                                                                aGTCTTCTCCTTGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCACTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTAcc                                   <  1:244146/149‑1 (MQ=255)
                                                                                                                            ggCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCCCCATGGTCTGCGCATGAATTTCTCGTCACACGGCCGCTTGCGAACGAATTTCGTGTACAATTGTTTCGaaa                       >  4:96596/1‑149 (MQ=255)
                                                                                                                                 cATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCGGCCCATGAATGTCTGGTCCCACGGCCACTTGAGAAACAATGTCGTTTACCATTGTTTCACAATATg                   >  4:338099/1‑148 (MQ=255)
                                                                                                                                  aTACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTa                 <  3:338099/149‑1 (MQ=255)
                                                                                                                                   tACAAAACCCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAg                <  6:120552/149‑1 (MQ=255)
                                                                                                                                    gaaaaaaCCCACTAGCAAGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTAACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGc               >  6:51318/3‑149 (MQ=255)
                                                                                                                                       aaaaCCCACTAGCAAGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCAc                                                                                             <  7:84725/68‑1 (MQ=255)
                                                                                                                                       aaaaCCCACTAGCAAGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCAc                                                                                             >  8:84725/1‑68 (MQ=255)
                                                                                                                                            ccACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGGCCCGTCCCCCCCTTGCACCATGGTGTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGc        >  8:94411/1‑148 (MQ=255)
                                                                                                                                               cTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAgg    <  3:364317/149‑1 (MQ=255)
                                                                                                                                                  gCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGCAGCCGTAAGCGAGGaa  <  2:365036/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGAAAGCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAGGAA  >  NC_002947/5549457‑5549750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑