Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,550,768 T→C 6.0% L337P (CTC→CCC)  PP_4881 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,550,7680TC 6.0% 195.3 / 2.8 67L337P (CTC→CCC) PP_4881iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (30/32);  new base C (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACTGGCTTATG  >  NC_002947/5550624‑5550895
                                                                                                                                                |                                                                                                                               
ccaccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtga                                                                                                                             <  3:203898/149‑1 (MQ=255)
 caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                                                                                                            <  6:243200/149‑1 (MQ=255)
 caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCACCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                                                                                                            <  6:68670/149‑1 (MQ=255)
     gccgAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACt                                                                                                                        <  7:12925/149‑1 (MQ=255)
      ccgAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTg                                                                                                                       >  7:206358/1‑149 (MQ=255)
         aGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTgccg                                                                                                                    >  5:239738/1‑149 (MQ=255)
           tACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTgccgcc                                                                                                                  <  7:73980/149‑1 (MQ=255)
              ccACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTg                                                                                                               >  2:351329/1‑149 (MQ=255)
                aCTGCACAAGTGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc                                                                                                             <  7:125456/149‑1 (MQ=255)
                aCTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc                                                                                                             >  3:145517/1‑149 (MQ=255)
                 cTGCACAAGGGCAGGGTCTCTGAGCGCGTCCTGAAACCTTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTcc                                                                                                            <  4:293102/149‑1 (MQ=255)
                    cacaAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGaa                                                                                                         >  4:188311/1‑149 (MQ=255)
                    cacaAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCGCTGATGACTGCCGCCCTGTCCGaa                                                                                                         >  6:81511/1‑149 (MQ=255)
                       aaGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCg                                                                                                      <  7:245129/149‑1 (MQ=255)
                       aaGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCg                                                                                                      >  2:336939/1‑149 (MQ=255)
                           gCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCt                                                                                                                >  4:140732/1‑135 (MQ=255)
                           gCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCt                                                                                                                <  3:140732/135‑1 (MQ=255)
                             aTGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCg                                                                                                >  1:185765/1‑149 (MQ=255)
                               ggTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCgg                                                                                               >  1:79949/1‑148 (MQ=255)
                                gTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTa                                                                                             >  2:343520/1‑149 (MQ=255)
                                       aCCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTc                                                                                       <  1:351329/148‑1 (MQ=255)
                                       aCCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTc                                                                                       <  1:336939/148‑1 (MQ=255)
                                       aCCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCg                                                                                      <  4:145517/149‑1 (MQ=255)
                                                 gAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCg                                                                            >  1:208475/1‑149 (MQ=255)
                                                 gAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCg                                                                            >  2:2456/1‑149 (MQ=255)
                                                  aaaCCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGcc                                                                             <  6:239738/147‑1 (MQ=255)
                                                  aaaCCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCg                                                                            >  1:50096/1‑148 (MQ=255)
                                                    aCCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGc                                                                           >  2:203811/1‑147 (MQ=255)
                                                    aCCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCaa                                                                         <  2:185765/149‑1 (MQ=255)
                                                        ttCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAgcgc                                                                     <  8:206358/149‑1 (MQ=255)
                                                        ttCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAgcgc                                                                     >  3:328101/1‑149 (MQ=255)
                                                           gacgaGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGt                                                                                              >  7:121467/1‑121 (MQ=255)
                                                           gacgaGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGt                                                                                              <  8:121467/121‑1 (MQ=255)
                                                            acgaGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCtt                                                                 >  4:50571/1‑149 (MQ=255)
                                                                    cgcccgccGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTgg                                                         <  1:343520/149‑1 (MQ=255)
                                                                      cccgccGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCCCTGATGACTGCCGCCCTGTCCGAAc                                                                                                        >  3:155922/1‑100 (MQ=255)
                                                                      cccgccGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCCCTGATGACTGCCGCCCTGTCCGAAc                                                                                                        >  3:150372/1‑100 (MQ=255)
                                                                      cccgccGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCCCTGATGACTGCCGCCCTGTCCGAAc                                                                                                        <  4:150372/100‑1 (MQ=255)
                                                                      cccgccGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCCCTGATGACTGCCGCCCTGTCCGAAc                                                                                                        <  4:155922/100‑1 (MQ=255)
                                                                        cgccgAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAg                                                     >  6:74983/1‑149 (MQ=255)
                                                                        cgccgAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAg                                                     >  8:89559/1‑149 (MQ=255)
                                                                           cgAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGtgc                                                  >  1:338875/1‑149 (MQ=255)
                                                                                  ctcGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCtgt                                           >  2:306172/1‑149 (MQ=255)
                                                                                     gCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCtgtg                                          <  3:50571/147‑1 (MQ=255)
                                                                                     gCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCt                                             <  4:328101/144‑1 (MQ=255)
                                                                                              ccTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCtgtg                                          >  2:272354/1‑138 (MQ=255)
                                                                                              ccTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCtgtg                                          <  1:272354/138‑1 (MQ=255)
                                                                                              ccTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGt                               <  3:188311/149‑1 (MQ=255)
                                                                                               cTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTg                              <  5:74983/149‑1 (MQ=255)
                                                                                               cTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTg                              >  7:300756/1‑149 (MQ=255)
                                                                                               cTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGACGGCCTGCTCTGATGACTCCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCCAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTg                              >  7:222464/1‑149 (MQ=255)
                                                                                                  ggCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTgg                             <  2:50096/147‑1 (MQ=255)
                                                                                                    cAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAAt                         <  7:89559/149‑1 (MQ=255)
                                                                                                    cAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAAt                         >  1:137775/1‑149 (MQ=255)
                                                                                                    cAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAAt                         <  2:79949/149‑1 (MQ=255)
                                                                                                        gCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGc                     >  5:336060/1‑149 (MQ=255)
                                                                                                        accGAAGAACCCATCGAAATTGAGCATGAGGTAGGCCTGATCTGATGACAGCCGCCATGTCCGAACCGGTGCCGGTACGCTACGTAACGACACGAAAGCGCCCATCCATCTGGCTAGTGCTGCCTGTGCTGTTCCTAGAGGAAAt                         >  8:254449/2‑145 (MQ=255)
                                                                                                         ccGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGc                     <  1:2456/148‑1 (MQ=255)
                                                                                                          cGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCtt                   <  1:306172/149‑1 (MQ=255)
                                                                                                             ggAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTtgct                <  8:300756/149‑1 (MQ=255)
                                                                                                              gAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCAGCCCTGTCCGAAGGGGTGGCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTtgct                <  8:222464/148‑1 (MQ=255)
                                                                                                                   cATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACt          <  1:203811/149‑1 (MQ=255)
                                                                                                                       gAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACTGGCt      <  2:338875/149‑1 (MQ=255)
                                                                                                                           ttGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACTGGCTTAtt  >  6:265762/1‑148 (MQ=255)
                                                                                                                           ttGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACTGGCTTATg  >  4:57401/1‑149 (MQ=255)
                                                                                                                             gAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACTGGCtt     <  4:175919/144‑1 (MQ=255)
                                                                                                                             gAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACTGGCtt     >  3:175919/1‑144 (MQ=255)
                                                                                                                                                |                                                                                                                               
CCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTCGTACCGAGCCGCAAGCGCCCTTCCATCTGGGTAGTGCTGCCTGTGCTGTTCCTAGTGGCAATGAGCTTGCTGCCACTGGCTTATG  >  NC_002947/5550624‑5550895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑