Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,570,178 G→C 7.1% A24G (GCA→GGA) ‡ nnrD ← ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,570,1780GC 7.1% 215.4 / 6.4 70A24G (GCA→GGA) ‡nnrDADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (31/34);  new base C (3/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

TGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGT  >  NC_002947/5570031‑5570317
                                                                                                                                                    |                                                                                                                                           
tGCGCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCtgccgtgc                                                                                                                                            <  2:338222/149‑1 (MQ=255)
          gggTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCgg                                                                                                                                  >  7:225764/1‑149 (MQ=255)
          gggTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCgg                                                                                                                                  <  5:65761/149‑1 (MQ=255)
            gTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCggg                                                                                                                                 >  2:18716/1‑148 (MQ=255)
             ttGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGc                                                                                                                                >  6:2910/1‑148 (MQ=255)
             ttGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGc                                                                                                                                >  2:64185/1‑148 (MQ=255)
                 cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCg                                                                                                                           >  4:246345/1‑149 (MQ=255)
                 cACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCg                                                                                                                           >  8:262796/1‑149 (MQ=255)
                  aCGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCg                                                                                                                           <  1:133311/148‑1 (MQ=255)
                    gCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCa                                                                                                                        <  6:365563/149‑1 (MQ=255)
                        aCCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgc                                                                                                                     <  1:292923/148‑1 (MQ=255)
                          cAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTAGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTATGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgc                                                                                                                  >  1:28702/1‑149 (MQ=255)
                           aGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGc                                                                                                                                    <  1:6728/130‑1 (MQ=255)
                           aGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGc                                                                                                                                    >  2:6728/1‑130 (MQ=255)
                             cccGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGa                                                                                                               <  3:253566/149‑1 (MQ=255)
                                     cgcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAAtt                                                                                                         >  6:334095/1‑147 (MQ=255)
                                      gcAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATtgtg                                                                                                      >  8:290429/1‑149 (MQ=255)
                                       cAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTgg                                                                                                     <  2:81813/149‑1 (MQ=255)
                                        aaCGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTggg                                                                                                    <  2:276943/149‑1 (MQ=255)
                                           gCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCa                                                                                                 <  5:2910/149‑1 (MQ=255)
                                               ggcggcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgct                                                                                                                 <  6:62198/129‑1 (MQ=255)
                                               ggcggcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACgctgct                                                                                                                 >  5:62198/1‑129 (MQ=255)
                                                gcggcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACt                                                                                            <  7:290429/149‑1 (MQ=255)
                                                 cggcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTg                                                                                           >  7:182919/1‑149 (MQ=255)
                                                 cggcTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTg                                                                                           <  5:334095/149‑1 (MQ=255)
                                                    cTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgggg                                                                                        <  7:262796/149‑1 (MQ=255)
                                                    cTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGc                                                                                                                                    <  6:57443/105‑1 (MQ=255)
                                                    cTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGGCACGGCAGGc                                                                                                                                    >  5:57443/1‑105 (MQ=255)
                                                        ggCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGtt                                                                                    <  1:18716/149‑1 (MQ=255)
                                                         gCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg                                                                                          <  8:284018/142‑1 (MQ=255)
                                                         gCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTgg                                                                                          >  7:284018/1‑142 (MQ=255)
                                                         gCACTGAGCAACACAGCGCCTCCGGTA‑CCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTg                                                                                  >  2:113651/1‑149 (MQ=255)
                                                             tGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                         >  6:323176/1‑139 (MQ=255)
                                                             tGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                         <  5:323176/139‑1 (MQ=255)
                                                             tGAGCAACACAGCGCCTCCGGTACCAAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTc                                                                               >  4:99789/1‑149 (MQ=255)
                                                               aGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTg                                                                             >  4:204418/1‑149 (MQ=255)
                                                                 cAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCg                                                                           >  5:193977/1‑149 (MQ=255)
                                                                 cAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCg                                                                           <  3:246345/149‑1 (MQ=255)
                                                                   acacAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCGTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACGGTCACGCTGCGGAGCAATTGTGGGGGATGACTGGGGTGGTTGGTCTGCggg                                                                         >  4:2738/1‑148 (MQ=255)
                                                                    cacaGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                         <  2:319120/132‑1 (MQ=255)
                                                                    cacaGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                         >  1:319120/1‑132 (MQ=255)
                                                                      gaGCGCCTCGGTTACCCTGGTCGCCCCAGAACAACATTATTTGCAAATTGCTCCCTTTTGTGGGTATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCAt                                                                       <  1:119665/148‑1 (MQ=255)
                                                                      caGCGCATCCGGTAACCAGGTCGACCCCGACCACCAGTACATGGCCAAAG‑TCGCATTTGTGGGCATGCACCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATg                                                                      <  6:257087/149‑1 (MQ=255)
                                                                        ccgccgcCCGTCCCCAGGTCGCCCCCGACCACCACTACATGGCCAAAG‑TCGCCGTTGTGGGCATGCGCCTGCCGGGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATggg                                                                    <  8:123763/148‑1 (MQ=255)
                                                                           ccTCCGGTACCCAGGTCGCCCCCGACGACCTGTACATGGCCAAAG‑TCGCCTTTATGGGGATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGGATTACTGGGGTGTTTGGTCTGCGGCATGGGGTc                                                                 <  2:28702/149‑1 (MQ=255)
                                                                                    cccAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTc                                                         >  4:229140/1‑148 (MQ=255)
                                                                                            gCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCt                                                 <  1:113651/148‑1 (MQ=255)
                                                                                             cccccGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                               <  8:225764/149‑1 (MQ=255)
                                                                                             cccccGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTgg                                               <  3:229140/149‑1 (MQ=255)
                                                                                              ccccGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTc                                                  >  1:344012/1‑145 (MQ=255)
                                                                                              ccccGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTc                                                  <  2:344012/145‑1 (MQ=255)
                                                                                               cccGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCa                                             <  6:193977/149‑1 (MQ=255)
                                                                                                ccGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCt                                                 <  4:144603/144‑1 (MQ=255)
                                                                                                ccGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCt                                                 >  3:144603/1‑144 (MQ=255)
                                                                                                        caGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATAc                                    <  1:169812/149‑1 (MQ=255)
                                                                                                                   cAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGcc                         >  7:228155/1‑149 (MQ=255)
                                                                                                                   cAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGAAGAATTATACGCACCTGAGcc                         >  4:272389/1‑149 (MQ=255)
                                                                                                                     aaG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCg                       <  8:182919/149‑1 (MQ=255)
                                                                                                                            ccTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                         <  6:342194/77‑1 (MQ=255)
                                                                                                                            ccTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTggg                                                                                         >  5:342194/1‑77 (MQ=255)
                                                                                                                             cTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGCGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGGAttgcc                >  5:114252/1‑149 (MQ=255)
                                                                                                                             cTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGc                                  >  3:162030/1‑131 (MQ=255)
                                                                                                                             cTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGc                                  <  4:162030/131‑1 (MQ=255)
                                                                                                                              tttGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcct               >  8:192319/1‑149 (MQ=255)
                                                                                                                                tgtgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgcctt              <  3:99789/148‑1 (MQ=255)
                                                                                                                                 gtgGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTAttgccttgc            >  5:247569/1‑149 (MQ=255)
                                                                                                                                      cATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatc       <  6:247569/149‑1 (MQ=255)
                                                                                                                                         ccgcCTGCCGGCACGGCAGGCGGGCAACCGACACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatccat    >  6:160011/2‑149 (MQ=255)
                                                                                                                                           gcCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcca     <  5:270579/146‑1 (MQ=255)
                                                                                                                                           gcCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCccatcca     >  6:270579/1‑146 (MQ=255)
                                                                                                                                           gcCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATga  >  5:82245/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
TGCTCGGGCCGGGTTGCCACGCTGACCAGCCCGGCGCCGCAACGCAGGGCGGCTTCGGCACTGAGCAACACAGCGCCTCCGGTACCCAGGTCGCCCCCGACCACCAGTACATGGCCAAAG‑TCGCCTTTGTGGGCATGCGCCTGCCGTGCCGGCAGGCGGGCAACCGTCACGCTGCTGAGCAATTGTGGGGCATTACTGGGGTGTTTGGTCTGCGGCATGGGGTCAAAGGCTCCAATGTCTGGCAGAATTATACGCACCTGAGCCCGTATTGCCTTGCCCATCCATGT  >  NC_002947/5570031‑5570317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑