Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 408,625 T→C 8.0% Y680C (TAC→TGC)  aceE ← pyruvate dehydrogenase E1 component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947408,6250TC 8.0% 139.4 / 2.8 51Y680C (TAC→TGC) aceEpyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCCATCATGTGGCTGTGACCGTCTTCGTGCTG  >  NC_002947/408479‑408770
                                                                                                                                                  |                                                                                                                                                  
gcAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATgtag                                                                                                                                                  >  4:332762/1‑149 (MQ=255)
     aTGGTGCCGGAGCCCATCAGCTGTACGTGGTGGGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTagaa                                                                                                                                             <  7:288579/149‑1 (MQ=255)
      tGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGtagtag                                                                                                                                               >  3:208671/1‑146 (MQ=255)
        gTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCGTCACGGTGATGTAGTagaaga                                                                                                                                           <  3:71384/148‑1 (MQ=255)
        gTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTagaaga                                                                                                                                           <  6:51793/148‑1 (MQ=255)
         tGCCGGAGCCCATAAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGGTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATg                                                                                                                                         >  3:76045/1‑149 (MQ=255)
               aGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGt                                                                                                                                   <  3:332762/149‑1 (MQ=255)
                gCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGtt                                                                                                                                  <  1:251931/149‑1 (MQ=255)
                gCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGtt                                                                                                                                  <  6:175384/149‑1 (MQ=255)
                   cATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGct                                                                                                                               >  4:326700/1‑149 (MQ=255)
                         cTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCgg                                                                                                                         <  7:16897/149‑1 (MQ=255)
                            tACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGctctagatcgg                                                                                                                      >  1:129035/1‑142 (MQ=255)
                            tACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGctc                                                                                                                              <  2:129035/141‑1 (MQ=255)
                            tACGTGGTGTGCGGCTTCGCGGGTGTCCTCTACCAGCAGGTACATGCCCTTGATGATGCATTCCTAGACACCGGCCTGCATGGCTGGCTTATCGTAGGATTCTTACATCAAGGTGATCTAGTAGAACATGTCCTTGTGGTCTTCGGTC‑a                                                                                                                     >  6:371001/1‑149 (MQ=255)
                                  gtgtGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑Atcttc                                                                                                                <  4:208671/148‑1 (MQ=255)
                                    gtgCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCttcttc                                                                                                             <  3:326700/149‑1 (MQ=255)
                                                     tCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTagaag                                                                                                                                            <  4:185221/102‑1 (MQ=255)
                                                     tCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTagaag                                                                                                                                            >  3:185221/1‑102 (MQ=255)
                                                                 agGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTgg                                                                                                 >  4:129412/1‑132 (MQ=255)
                                                                 agGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTgg                                                                                                 <  3:129412/132‑1 (MQ=255)
                                                                    tACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCg                                                                               >  2:313864/1‑147 (MQ=255)
                                                                       aTGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGCTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGACAGCTCGTAGcc                                                                          >  1:310026/1‑149 (MQ=255)
                                                                           ccTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTc                                                                                <  7:153785/139‑1 (MQ=255)
                                                                           ccTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTc                                                                                >  8:153785/1‑139 (MQ=255)
                                                                           ccTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAg                                                                      <  5:74829/149‑1 (MQ=255)
                                                                             ttGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGt                                                                    >  2:211093/1‑149 (MQ=255)
                                                                               gatgatGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCg                                                                  >  8:82519/1‑149 (MQ=255)
                                                                                     gCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCTTAGGTCGGATCg                                                             >  6:93780/1‑148 (MQ=255)
                                                                                       cTTCCTCGACCCCGCCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCTAGCTCGTAGCCGTAGGGCGGATCGTAg                                                          <  6:240572/149‑1 (MQ=255)
                                                                                        ttCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAgg                                                         >  7:63449/1‑149 (MQ=255)
                                                                                          ccTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAgg                                                         <  2:85573/147‑1 (MQ=255)
                                                                                           cTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTagaaga                                                                                                                                           <  1:170420/65‑1 (MQ=255)
                                                                                           cTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTagaaga                                                                                                                                           >  2:170420/1‑65 (MQ=255)
                                                                                            tCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCg                                                     >  7:230109/1‑149 (MQ=255)
                                                                                            tCGACACCGGCCGGCATGGCTGGCTGCTAGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCg                                                     >  8:125842/1‑149 (MQ=255)
                                                                                              gACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCgg                                                    >  6:33064/1‑148 (MQ=255)
                                                                                              gACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGCAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCgg                                                    >  6:12715/1‑148 (MQ=255)
                                                                                              gACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGCAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCgg                                                    >  2:210727/1‑148 (MQ=255)
                                                                                                   cggccggcATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGCAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTc                                              <  1:210727/149‑1 (MQ=255)
                                                                                                   cggccggcATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGCAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTc                                              <  5:12715/149‑1 (MQ=255)
                                                                                                   cggccggcATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGAAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTc                                              >  1:265975/1‑149 (MQ=255)
                                                                                                     gccggcATGGCTGGCTGCTAGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCgg                                            <  7:125842/149‑1 (MQ=255)
                                                                                                         gcATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATg                                        <  1:211093/149‑1 (MQ=255)
                                                                                                          cATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGt                                                            >  4:95990/1‑128 (MQ=255)
                                                                                                          cATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGt                                                            <  3:95990/128‑1 (MQ=255)
                                                                                                              gctggctgCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGc                                    <  8:63449/148‑1 (MQ=255)
                                                                                                                     gctgGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCcat                             >  3:211378/1‑148 (MQ=255)
                                                                                                                      ctgGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTTTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACAGCCAGCTAGTCTCCGGAGGTCTGATCTGAGGTGCGGCCGTTCGGGAGTGTGCCCGCcatca                           >  6:96005/1‑149 (MQ=255)
                                                                                                                              aTTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCAGTCCTGGATGATCACCGACAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGAAGTTCGGGATGGTGCCCGCCATCATGTGGCtg                   >  2:52253/1‑149 (MQ=255)
                                                                                                                               ttCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTCGATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCCATCATGTGGCtg                   >  1:271445/1‑149 (MQ=255)
                                                                                                                                  gTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCCATCATGTGGCTGTGa                <  8:230109/148‑1 (MQ=255)
                                                                                                                                  gTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCCATCATGTGGCTGTGAc               <  2:271445/149‑1 (MQ=255)
                                                                                                                                       tcaCGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCCATCATGTGGCTGTGACCGTCt          >  2:164244/1‑149 (MQ=255)
                                                                                                                                                 gtagtaGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCCATCATGTGGCTGTGACCGTCTTCGtgctg  >  6:159395/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCAGGATGGTGCCGGAGCCCATCAGCTGTACGTGGTGTGCGGCTTCGCGGGTGTCCTCTTCCAGCAGGTACATGCCCTTGATGATGCCTTCCTCGACACCGGCCGGCATGGCTGGCTGCTGGTAGGATTCGTTCATCACGGTGATGTAGTAGAAGATGTCCTGTTGCTCTTCGGTC‑ATCTTCTTCATGCCGTCCTGGATGATCACCGCCAGCTCGTAGCCGTAGGTCGGATCGTAGGTGCGGCAGTTCGGGATGGTGCCCGCCATCATGTGGCTGTGACCGTCTTCGTGCTG  >  NC_002947/408479‑408770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑