Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,582,822	T→A	10.2%	E175V (GAG→GTG)	PP_4911 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,582,822	0	T	A	10.2%	179.9 / 10.5	69	E175V (GAG→GTG)	PP_4911	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base A (3/4);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

TGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCGGTC  >  NC_002947/5582677‑5582968                                                                                                                                                   |                                                                                                                                                   tGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGa                                                                                                                                                 <  8:342819/149‑1 (MQ=255)    aGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGAGCAGCAGCACGAggg                                                                                                                                              <  4:65792/149‑2 (MQ=255)    aGCGGGCGTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGt                                                                                                                                              <  7:273671/149‑1 (MQ=255)           tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGACGTGgatga                                                                                                                                        >  2:110052/1‑148 (MQ=255)                    gcatctTCGGCAATCGCCGGCTGGTTGGCCAGAGGCTTCACAGCCGTGCTCAGCTGTTCGGCCAGCTTATGCAAAAGTTGGACCTGAGCCTGAACGTGCG‑ACGACTCACTGCCCTCGTTCTGAGGCTCGAGGTGGATCATACGGTTGt                                                                                                                               >  4:140829/4‑148 (MQ=255)                       tctTCGGCAATCGCCGGCTGGTTGGCCAGCCGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACg                                                                                                                           >  1:30380/1‑149 (MQ=255)                        ctTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTACCTGCCCGCGTGCTGCTGCTGGAGGTGGATGATACGGTTGTCACGc                                                                                                                          >  8:119806/1‑149 (MQ=255)                         ttCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCa                                                                                                                             >  3:37133/1‑145 (MQ=255)                         ttCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCa                                                                                                                             <  4:37133/145‑1 (MQ=255)                           cGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGc                                                                                                                          <  7:230570/146‑1 (MQ=255)                                aTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATgg                                                                                                                    <  5:332756/147‑1 (MQ=255)                                aTCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATgg                                                                                                                    >  6:332756/1‑147 (MQ=255)                                 tCGCCGGCTGGTTGGCAAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCa                                                                                                                 <  5:230414/149‑1 (MQ=255)                                     cGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGCTCGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcgg                                                                                                              <  1:110052/149‑1 (MQ=255)                                           gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCaa                                                                                                       <  2:205890/149‑1 (MQ=255)                                           gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACCGTTGTCACGCACATGGCCACGGCGGTc                                                                                                         >  3:280591/1‑147 (MQ=255)                                               gCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCa                                                                                                    <  5:190075/148‑1 (MQ=255)                                                ccAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGa                                                                                                  >  4:254949/1‑149 (MQ=255)                                                  aGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACg                                                                                                >  2:10223/1‑149 (MQ=255)                                                           aCCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTggg                                                                                        >  5:194796/1‑148 (MQ=255)                                                            ccgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATgg                                                                                                                    >  6:94001/1‑119 (MQ=255)                                                            ccgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATgg                                                                                                                    <  5:94001/119‑1 (MQ=255)                                                            ccgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCa                                                                                      <  4:271643/149‑1 (MQ=255)                                                               ccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTGGAGGTGGATGCTACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATc                                                                                    >  3:223915/1‑148 (MQ=255)                                                                cgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCa                                                                                  <  3:254949/149‑1 (MQ=255)                                                                  tGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAg                                                                                 >  5:266265/1‑148 (MQ=255)                                                                     tCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATg                                                                             >  5:291211/1‑149 (MQ=255)                                                                            ttCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGAGACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGc                                                                           >  7:193309/1‑144 (MQ=255)                                                                            ttCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGAGACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGc                                                                           <  8:193309/144‑1 (MQ=255)                                                                               ggCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGt                                                                   >  4:262894/1‑149 (MQ=255)                                                                                gCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGtt                                                                  <  3:29246/149‑1 (MQ=255)                                                                                gCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGtt                                                                  <  7:119806/149‑1 (MQ=255)                                                                                 ccatgttCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTc                                                                 <  3:140829/144‑1 (MQ=255)                                                                                 ccAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTc                                                                 >  6:114344/1‑149 (MQ=255)                                                                                   aGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCGAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCtt                                                               >  8:226931/1‑149 (MQ=255)                                                                                   aGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCtt                                                               <  6:291211/149‑1 (MQ=255)                                                                                    gCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGCCTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTg                                                              >  6:197780/1‑149 (MQ=255)                                                                                           cAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGc                                                             <  6:339829/143‑1 (MQ=255)                                                                                           cAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGc                                                             >  5:339829/1‑143 (MQ=255)                                                                                            aaCAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCATTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCa                                                           <  3:115356/144‑1 (MQ=255)                                                                                            aaCAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCATTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCa                                                           >  4:115356/1‑144 (MQ=255)                                                                                             aCAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATcg                                                     >  8:230678/1‑148 (MQ=255)                                                                                              cAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCa                                                                                  >  8:118674/1‑119 (MQ=255)                                                                                              cAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCa                                                                                  <  7:118674/119‑1 (MQ=255)                                                                                               aGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAg                                                   >  8:1959/1‑149 (MQ=255)                                                                                                 ttGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGc                                                 <  1:144133/149‑1 (MQ=255)                                                                                                    gCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtg                                              <  6:82650/149‑1 (MQ=255)                                                                                                     cccTGAGCCTGCACCTGCC‑ACGCCTCACTCCCCTCGTGCTCCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGCCCACGGCCGACAAGCAGACGCCACTGGGCATCCAGGATCCCCGCCTCGTTCTTGCCAGAATCCAGGCgtgt                                             >  4:157918/1‑149 (MQ=255)                                                                                                      ccTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGGCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtgt                                             >  5:90364/1‑148 (MQ=255)                                                                                                                ctccTGCG‑ACGACTCACTGGCCTGGTGCTGCTGCTCGTGGTGGTTGATTCGGTTGTATCGCTCATGGCCACGGCGGTGAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGacaaca                                  <  3:157918/147‑1 (MQ=255)                                                                                                                   cTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGc                                                                                                     <  3:279699/79‑1 (MQ=255)                                                                                                                   cTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGc                                                                                                     >  4:279699/1‑79 (MQ=255)                                                                                                                         acgacTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGATGTCACGCACAAGGCCACGGCCGTCAAGCAGACGCCACTCGGCATCCAGGAAGGCCGCCTCGTTCTTGCCAGAATCCAGGCGAGTGATCGACAACAGCACTTg                           >  5:62153/1‑148 (MQ=255)                                                                                                                           gacTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCAc                        >  2:45201/1‑149 (MQ=255)                                                                                                                              tCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCGACGGCGGTCAAGCAGACGCCACTGGGCATACAGGATGGCCGGCTTGTTGTTGGCAGAATACAGGCGTGTGATCGACAACAGCAAATGCACGTc                     >  1:285550/1‑149 (MQ=255)                                                                                                                                aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGacaaca                                  <  7:210396/134‑1 (MQ=255)                                                                                                                                aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGACACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGacaaca                                  >  8:210396/1‑134 (MQ=255)                                                                                                                                 tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAg                                                                                 <  8:302228/82‑1 (MQ=38)                                                                                                                                 tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAg                                                                                 >  7:302228/5‑86 (MQ=38)                                                                                                                                 tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTcc                    <  4:17540/143‑1 (MQ=255)                                                                                                                                 tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTcc                    >  7:151414/5‑147 (MQ=255)                                                                                                                                 tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTcc                    >  3:17540/5‑147 (MQ=255)                                                                                                                                 tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTcc                    <  8:151414/143‑1 (MQ=255)                                                                                                                                 cTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCgg                  <  6:266265/149‑1 (MQ=255)                                                                                                                                    cccTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCt               <  6:194796/149‑1 (MQ=255)                                                                                                                                    cccTCGTGCTGCTGCTCGAGGTGGATGATACCGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCt               <  4:280591/149‑1 (MQ=255)                                                                                                                                     ccTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTg              <  2:30380/149‑1 (MQ=255)                                                                                                                                       tCGTGCTGCTGCTCGAGGTGGATGATACGGTTGACACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTg              >  2:288061/1‑147 (MQ=255)                                                                                                                                         gTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGc          <  1:45201/149‑1 (MQ=255)                                                                                                                                                  ctCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCgg                                                                        >  4:251417/1‑78 (MQ=255)                                                                                                                                                  ctCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCGGTc  <  3:262894/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TGCAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCG‑ACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGCCTGCGGTC  >  NC_002947/5582677‑5582968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑