Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,633,937 T→A 9.8% L1156Q (CTG→CAG)  putA → proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,633,9370TA 9.8% 260.1 / 14.1 91L1156Q (CTG→CAG) putAproline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (43/39);  new base A (5/4);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

ACCCGCCCGGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTC  >  NC_002947/5633789‑5634082
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aCCCGCCCGGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCt                                                                                                                                                    >  8:177867/1‑149 (MQ=255)
  ccgcccgGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGTACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCtgt                                                                                                                                                  >  2:76631/1‑149 (MQ=255)
  acgcccgGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCtgt                                                                                                                                                  <  5:162223/148‑1 (MQ=255)
        ggCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACGACCAGCTGGCCGACCTGGCCGCGCTGTGCAGcc                                                                                                                                            <  8:340650/149‑1 (MQ=255)
         gCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCa                                                                                                                                           <  2:331177/149‑1 (MQ=255)
           cGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGACCAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCTGCACAGCGCGGCCAgg                                                                                                                                         <  2:144026/149‑2 (MQ=255)
              cGCCATTGGCCGCCACTTCCAGCACCAGGACGGCGAAGGCACGCCGGACCGCACCCTGTACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCAGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTc                                                                                                                                       <  1:76631/149‑1 (MQ=255)
                 cATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gcca                                                                                                                                  <  8:109242/149‑1 (MQ=255)
                         gccACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gcca                                                                                                                                  <  8:123594/141‑1 (MQ=255)
                         gccACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gcca                                                                                                                                  >  7:123594/1‑141 (MQ=255)
                              ttCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCCCGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGGCGCagag                                                                                                                     >  2:310493/1‑149 (MQ=255)
                                 cagcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCg                                                                                                                   <  8:122006/148‑1 (MQ=255)
                                 cagcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGgccagctgg                                                                                                                             <  3:66262/138‑4 (MQ=25)
                                 cagcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGgccagctgg                                                                                                                             >  4:66262/1‑135 (MQ=25)
                                   gcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCa                                                                                                                >  6:98125/1‑149 (MQ=255)
                                        agGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATcgcc                                                                                                           >  2:155113/1‑149 (MQ=255)
                                           aCGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATcgcccgc                                                                                                        <  7:177867/149‑1 (MQ=255)
                                             ggCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCct                                                                                                      >  7:145023/1‑149 (MQ=255)
                                             ggCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCct                                                                                                      >  1:152703/1‑149 (MQ=255)
                                              gCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCctg                                                                                                     >  3:289519/1‑149 (MQ=255)
                                                gAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCctgct                                                                                                   >  7:134743/1‑149 (MQ=255)
                                                  aGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCtgctgc                                                                                                 <  2:170696/149‑1 (MQ=255)
                                                    gCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCa                                                                                                                                               >  7:292331/1‑102 (MQ=255)
                                                    gCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCa                                                                                                                                               <  8:292331/102‑1 (MQ=255)
                                                    gCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCtgctgc                                                                                                 >  1:298216/1‑147 (MQ=255)
                                                      aCGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAgg                                                                                             >  8:142095/1‑149 (MQ=255)
                                                      aCGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAgg                                                                                             >  2:84170/1‑149 (MQ=255)
                                                      aCGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAgg                                                                                             >  6:113596/1‑149 (MQ=255)
                                                       cGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGc                                                                                            <  1:238513/149‑1 (MQ=255)
                                                       cGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGc                                                                                            <  5:113596/149‑1 (MQ=255)
                                                            gACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGAc                                                                                       >  7:151480/1‑149 (MQ=255)
                                                                  aCCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGa                                                                                 <  2:152703/149‑1 (MQ=255)
                                                                  aCCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGa                                                                                 <  5:98125/149‑1 (MQ=255)
                                                                  aCCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGa                                                                                 <  1:155113/149‑1 (MQ=255)
                                                                    ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc                                                                               <  7:328339/149‑1 (MQ=255)
                                                                    ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc                                                                               <  1:23760/149‑1 (MQ=255)
                                                                    ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc                                                                               >  8:328339/1‑149 (MQ=255)
                                                                        cACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgcgc                                                                             <  2:343179/147‑1 (MQ=255)
                                                                         aCGACCCGCTGCGAACGCCGCTGCACGGCCTGAAGGCCGGGGCC‑GAGCACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCAGCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAAc                                                                          <  4:303153/149‑1 (MQ=255)
                                                                         aCGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCaa                                                                           >  5:265514/1‑148 (MQ=255)
                                                                         aCGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAAc                                                                          <  8:134743/149‑1 (MQ=255)
                                                                              cAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTa                                                                     >  7:104623/1‑149 (MQ=255)
                                                                               aGCTCGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTAC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTa                                                                     >  3:188552/1‑148 (MQ=255)
                                                                                 cTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTacac                                                                  >  2:190502/1‑149 (MQ=255)
                                                                                    gTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCAt                                                               >  7:290887/1‑149 (MQ=255)
                                                                                    gTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCAt                                                               >  7:285773/1‑149 (MQ=255)
                                                                                    gTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCAt                                                               <  7:142095/149‑1 (MQ=255)
                                                                                         gCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGc                                                          >  8:72894/1‑149 (MQ=255)
                                                                                             cTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCAGCTGCCAGGCCCGACCGCCGAGCGCAACAGCTCCACCATTCTGCcgcg                                                      >  2:309395/1‑149 (MQ=255)
                                                                                               gCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCcgc                                                       <  8:276365/146‑1 (MQ=255)
                                                                                               gCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCcgc                                                       >  7:276365/1‑146 (MQ=255)
                                                                                                 cggccatGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGa                                                   <  6:291713/142‑1 (MQ=255)
                                                                                                  cGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGc                                                 <  1:84170/149‑1 (MQ=255)
                                                                                                         aaGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCGTTCTGCCGCGTGAGCACGTGCt                                          >  6:353429/1‑149 (MQ=255)
                                                                                                              cTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGc                                                                       >  6:122471/1‑115 (MQ=255)
                                                                                                              cTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGc                                                                       <  5:122471/115‑1 (MQ=255)
                                                                                                              cTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGcc                                     >  1:275178/1‑149 (MQ=255)
                                                                                                               tGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTTGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCt                                    >  8:230162/1‑149 (MQ=255)
                                                                                                                   cc‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGcc                                <  8:28637/149‑1 (MQ=255)
                                                                                                                    c‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCAc                                               >  5:301876/1‑133 (MQ=255)
                                                                                                                    c‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCAc                                               <  6:301876/133‑1 (MQ=255)
                                                                                                                    c‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGcc                                >  7:111633/1‑148 (MQ=255)
                                                                                                                      gagaACAACCAGCTGGCCGACCTGGCCACGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGa                              >  1:166115/1‑149 (MQ=255)
                                                                                                                         aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt                                        >  1:355865/1‑136 (MQ=255)
                                                                                                                         aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt                                        <  2:355865/136‑1 (MQ=255)
                                                                                                                         aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt                                        <  2:361384/136‑1 (MQ=255)
                                                                                                                         aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt                                        >  1:361384/1‑136 (MQ=255)
                                                                                                                          acaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc                          <  1:190502/149‑1 (MQ=255)
                                                                                                                           cgactggCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGGCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTg                                   <  1:122339/133‑1 (MQ=25)
                                                                                                                           cgactggCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGGCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTg                                   >  2:122339/7‑139 (MQ=25)
                                                                                                                           caacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACg                         <  6:265514/149‑1 (MQ=255)
                                                                                                                            aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGt                                                     >  5:267359/1‑120 (MQ=255)
                                                                                                                            aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGt                                                     <  6:267359/120‑1 (MQ=255)
                                                                                                                            aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACg                         >  8:49582/1‑148 (MQ=255)
                                                                                                                              ccAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGaaa                      <  8:104623/149‑1 (MQ=255)
                                                                                                                                 gCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc                          >  4:192393/1‑142 (MQ=255)
                                                                                                                                 gCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc                          <  3:192393/142‑1 (MQ=255)
                                                                                                                                 gCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAAcc                    >  5:223408/1‑148 (MQ=255)
                                                                                                                                  cTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGa                  >  5:206588/1‑149 (MQ=255)
                                                                                                                                     gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAc                 <  2:275178/147‑1 (MQ=255)
                                                                                                                                     gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAc                 <  6:206588/147‑1 (MQ=255)
                                                                                                                                     gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACct               >  6:358010/1‑149 (MQ=255)
                                                                                                                                       cGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgc             >  3:285494/1‑149 (MQ=255)
                                                                                                                                         aacTGGCTGCACAGCGCGGCCAGGTCGGCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgct            >  7:45984/3‑149 (MQ=255)
                                                                                                                                         aCCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgctg           <  8:151480/149‑1 (MQ=255)
                                                                                                                                         aCCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACACCTACACCATTCTGCCGCGTGAGCACGTGCTGGGCCTGGCCGACCACGAGACCGACctgctg           >  8:283399/1‑149 (MQ=255)
                                                                                                                                          ccTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGGCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTgg          >  1:34025/1‑149 (MQ=255)
                                                                                                                                           cTGGCTGCACAGCGCGGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACaa                           <  5:272574/131‑1 (MQ=255)
                                                                                                                                           cTGGCTGCACAGCGCGGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACaa                           >  6:272574/1‑131 (MQ=255)
                                                                                                                                           cTGGCTGCACAGCGCGGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGgc         >  4:11403/1‑149 (MQ=255)
                                                                                                                                           cTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGt                                             >  4:133267/1‑113 (MQ=255)
                                                                                                                                           cTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGt                                             <  3:133267/113‑1 (MQ=255)
                                                                                                                                              gCCACGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGgcg        <  2:166115/147‑1 (MQ=255)
                                                                                                                                                  cgcTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTc  <  4:289519/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ACCCGCCCGGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTC  >  NC_002947/5633789‑5634082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑