| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,633,939 | T→C | 9.9% | C1157R (TGC→CGC) | putA → | proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,633,939 | 0 | T | C | 9.9% | 247.4 / 14.6 | 91 | C1157R (TGC→CGC) | putA | proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (43/39); new base C (5/4); total (48/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
CCGCCCGGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTCGCCG > NC_002947/5633791‑5634086 | ccgcccgGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGTACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCtgt > 2:76631/1‑149 (MQ=255)acgcccgGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCtgt < 5:162223/148‑1 (MQ=255) ggCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACGACCAGCTGGCCGACCTGGCCGCGCTGTGCAGcc < 8:340650/149‑1 (MQ=255) gCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCa < 2:331177/149‑1 (MQ=255) cGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGACCAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCTGCACAGCGCGGCCAgg < 2:144026/149‑2 (MQ=255) cGCCATTGGCCGCCACTTCCAGCACCAGGACGGCGAAGGCACGCCGGACCGCACCCTGTACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCCAGAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTc < 1:76631/149‑1 (MQ=255) cATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gcca < 8:109242/149‑1 (MQ=255) gccACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gcca > 7:123594/1‑141 (MQ=255) gccACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑gcca < 8:123594/141‑1 (MQ=255) ttCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCCCGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGGCGCagag > 2:310493/1‑149 (MQ=255) cagcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCg < 8:122006/148‑1 (MQ=255) cagcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGgccagctgg > 4:66262/1‑135 (MQ=25) cagcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGgccagctgg < 3:66262/138‑4 (MQ=25) gcagcagGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCa > 6:98125/1‑149 (MQ=255) agGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATcgcc > 2:155113/1‑149 (MQ=255) aCGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATcgcccgc < 7:177867/149‑1 (MQ=255) ggCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCct > 1:152703/1‑149 (MQ=255) ggCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCct > 7:145023/1‑149 (MQ=255) gCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCctg > 3:289519/1‑149 (MQ=255) gAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCctgct > 7:134743/1‑149 (MQ=255) aGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCtgctgc < 2:170696/149‑1 (MQ=255) gCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCa > 7:292331/1‑102 (MQ=255) gCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCa < 8:292331/102‑1 (MQ=255) gCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCtgctgc > 1:298216/1‑147 (MQ=255) aCGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAgg > 6:113596/1‑149 (MQ=255) aCGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAgg > 8:142095/1‑149 (MQ=255) aCGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAgg > 2:84170/1‑149 (MQ=255) cGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGc < 5:113596/149‑1 (MQ=255) cGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGc < 1:238513/149‑1 (MQ=255) gACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGAc > 7:151480/1‑149 (MQ=255) aCCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGa < 5:98125/149‑1 (MQ=255) aCCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGa < 2:152703/149‑1 (MQ=255) aCCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGa < 1:155113/149‑1 (MQ=255) ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc < 7:328339/149‑1 (MQ=255) ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc < 1:23760/149‑1 (MQ=255) ccTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgc > 8:328339/1‑149 (MQ=255) cACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAgcgc < 2:343179/147‑1 (MQ=255) aCGACCCGCTGCGAACGCCGCTGCACGGCCTGAAGGCCGGGGCC‑GAGCACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCAGCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAAc < 4:303153/149‑1 (MQ=255) aCGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCaa > 5:265514/1‑148 (MQ=255) aCGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAAc < 8:134743/149‑1 (MQ=255) cAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTa > 7:104623/1‑149 (MQ=255) aGCTCGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTAC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTa > 3:188552/1‑148 (MQ=255) cTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTacac > 2:190502/1‑149 (MQ=255) gTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCAt > 7:290887/1‑149 (MQ=255) gTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCAt < 7:142095/149‑1 (MQ=255) gTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCAt > 7:285773/1‑149 (MQ=255) gCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGc > 8:72894/1‑149 (MQ=255) cTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCAGCTGCCAGGCCCGACCGCCGAGCGCAACAGCTCCACCATTCTGCcgcg > 2:309395/1‑149 (MQ=255) gCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCcgc < 8:276365/146‑1 (MQ=255) gCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCcgc > 7:276365/1‑146 (MQ=255) cggccatGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGa < 6:291713/142‑1 (MQ=255) cGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGc < 1:84170/149‑1 (MQ=255) aaGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCGTTCTGCCGCGTGAGCACGTGCt > 6:353429/1‑149 (MQ=255) cTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGc > 6:122471/1‑115 (MQ=255) cTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGc < 5:122471/115‑1 (MQ=255) cTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGcc > 1:275178/1‑149 (MQ=255) tGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTTGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCt > 8:230162/1‑149 (MQ=255) cc‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGcc < 8:28637/149‑1 (MQ=255) c‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCAc > 5:301876/1‑133 (MQ=255) c‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCAc < 6:301876/133‑1 (MQ=255) c‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGcc > 7:111633/1‑148 (MQ=255) gagaACAACCAGCTGGCCGACCTGGCCACGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGa > 1:166115/1‑149 (MQ=255) aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt < 2:361384/136‑1 (MQ=255) aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt < 2:355865/136‑1 (MQ=255) aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt > 1:355865/1‑136 (MQ=255) aacaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCtgt > 1:361384/1‑136 (MQ=255) acaacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc < 1:190502/149‑1 (MQ=255) cgactggCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGGCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTg < 1:122339/133‑1 (MQ=25) cgactggCTGG‑CGAACTGGCTGCACAGCGCGGCCAGGTCGGCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTg > 2:122339/7‑139 (MQ=25) caacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACg < 6:265514/149‑1 (MQ=255) aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGt < 6:267359/120‑1 (MQ=255) aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGt > 5:267359/1‑120 (MQ=255) aacCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACg > 8:49582/1‑148 (MQ=255) ccAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGaaa < 8:104623/149‑1 (MQ=255) gCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc > 4:192393/1‑142 (MQ=255) gCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAAc < 3:192393/142‑1 (MQ=255) gCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAAcc > 5:223408/1‑148 (MQ=255) cTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGa > 5:206588/1‑149 (MQ=255) gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAc < 2:275178/147‑1 (MQ=255) gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGAc < 6:206588/147‑1 (MQ=255) gCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACct > 6:358010/1‑149 (MQ=255) cGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgc > 3:285494/1‑149 (MQ=255) aacTGGCTGCACAGCGCGGCCAGGTCGGCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgct > 7:45984/3‑149 (MQ=255) aCCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACctgctg < 8:151480/149‑1 (MQ=255) aCCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACACCTACACCATTCTGCCGCGTGAGCACGTGCTGGGCCTGGCCGACCACGAGACCGACctgctg > 8:283399/1‑149 (MQ=255) ccTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGGCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTgg > 1:34025/1‑149 (MQ=255) cTGGCTGCACAGCGCGGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACaa < 5:272574/131‑1 (MQ=255) cTGGCTGCACAGCGCGGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACaa > 6:272574/1‑131 (MQ=255) cTGGCTGCACAGCGCGGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGgc > 4:11403/1‑149 (MQ=255) cTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGt < 3:133267/113‑1 (MQ=255) cTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGt > 4:133267/1‑113 (MQ=255) gCCACGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGgcg < 2:166115/147‑1 (MQ=255) cgcTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTc < 4:289519/149‑1 (MQ=255) gtgCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTcgccg > 8:92509/1‑149 (MQ=255) | CCGCCCGGCCGACGCCATTGGCCGCCACTTCCAGCAGCAGGACGGCGAAGGCACGCCGGACCGCACCCTGCACGAACAGCTGGTAAAGCCGCTGCACGGCCTGAAGGCCTGGGCC‑GAGAACAACCAGCTGGCCGACCTGGCCGCGCTGTGCAGCCAGTTC‑GCCAGCCAGTCGCAGAGCGGCATCGCCCGCCTGCTGCCAGGCCCGACCGGCGAGCGCAACAGCTACACCATTCTGCCGCGTGAGCACGTGCTGTGCCTGGCCGACAACGAAACCGACCTGCTGGCGCAGTTCGCCG > NC_002947/5633791‑5634086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |