Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 110,223 C→T 7.5% G81G (GGC→GGT)  ctaD → cytochrome c oxidase subunit 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947110,2230CT 7.5% 155.2 / 2.2 53G81G (GGC→GGT) ctaDcytochrome c oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base C (25/24);  new base T (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

AAGACATCGGCACGATGTACCTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCTTCCTGCTGCTG  >  NC_002947/110075‑110370
                                                                                                                                                    |                                                                                                                                                    
aaGACATCGGCACGATGTACCTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGc                                                                                                                                                      <  3:368186/149‑1 (MQ=255)
 aGACATCGGCACGATGTACCTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGcc                                                                                                                                                     >  4:320477/1‑149 (MQ=255)
 aGACATCGGCACGATGTACCTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGcc                                                                                                                                                     >  6:242982/1‑149 (MQ=255)
             gATGTACCTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGgt                                                                                                                                           <  5:149658/147‑1 (MQ=255)
             gATGTACCTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGgt                                                                                                                                           >  6:149658/1‑147 (MQ=255)
              aTGTACCTGTGGGTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGtt                                                                                                                                        <  6:1700/149‑1 (MQ=255)
                   ccTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGAGCGTGGAGCCGGCGTGCTTCAACCAGATGACCAGCATGCATGTCCTTCTCAGGGTGTGTGgcg                                                                                                                                   >  8:342415/1‑149 (MQ=255)
                       tgcttCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGGGAGCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGt                                                                                                                               <  6:111988/146‑1 (MQ=255)
                       tgGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGt                                                                                                                               <  5:242982/149‑1 (MQ=255)
                         gTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTg                                                                                                                              <  3:320477/148‑1 (MQ=255)
                         gTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTCTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGa                                                                                                                             >  4:150931/1‑149 (MQ=255)
                          ttCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATc                                                                                                                                                >  1:8948/1‑129 (MQ=255)
                          ttCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATc                                                                                                                                                <  2:8948/129‑1 (MQ=255)
                          ttCAGTGGCCTCCTGTTCCTGCTCGGTCGTTCGTCTGCCAGGGCGATCCGCGCTGAGCTGTTCCAGCCGGGTGGGCAGATCGTGGCGCCGGCGCTCTTCACCCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGAt                                                                                                                            <  6:336823/149‑1 (MQ=255)
                           tCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGTCTGATCATGGTGTTTGGCGCGGt                                                                                                                               <  8:178286/145‑1 (MQ=255)
                           tCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGTCTGATCATGGTGTTTGGCGCGGt                                                                                                                               >  7:178286/1‑145 (MQ=255)
                              gTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCGGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCg                                                                                                                        <  7:304515/149‑1 (MQ=255)
                                      atGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑Ttcgt                                                                                                               <  2:39159/149‑1 (MQ=255)
                                               cTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGCTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTg                                                                                                        <  7:334495/147‑1 (MQ=255)
                                                tCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAAGCAGATGACCACAATGCATGGCATGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTATTCGGCCTgtc                                                                                                      >  5:314187/1‑146 (MQ=255)
                                                      gCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTACAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑CTCGTCGGCCTGGCCAACTg                                                                                                >  3:269087/1‑148 (MQ=255)
                                                        tCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGgat                                                                                             >  3:307666/1‑149 (MQ=255)
                                                              gCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTgg                                                                                                       <  3:285806/133‑1 (MQ=255)
                                                              gCCATGGTGATCCGCGATGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTgg                                                                                                       >  4:285806/1‑133 (MQ=255)
                                                                      gATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCtgatga                                                                               >  3:310016/1‑149 (MQ=255)
                                                                       aTCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTgatgat                                                                              >  2:274971/1‑149 (MQ=255)
                                                                         ccGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCg                                                                            >  3:299235/1‑149 (MQ=255)
                                                                          cgcgCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCgg                                                                           >  6:118336/1‑149 (MQ=255)
                                                                           gcgcTGAGCTGTTCCAGCCGGGTTTACAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑CTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGgc                                                                          <  4:269087/149‑1 (MQ=255)
                                                                                 aGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGccc                                                                    >  3:111919/1‑149 (MQ=255)
                                                                                  gCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGccc                                                                    >  1:200054/1‑148 (MQ=255)
                                                                                    tGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGa                                                                  >  3:294448/1‑148 (MQ=255)
                                                                                        ccAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATg                                                              <  3:150931/148‑1 (MQ=255)
                                                                                         cAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATgg                                                             <  4:111919/148‑1 (MQ=255)
                                                                                         cAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGc                                                            <  3:138680/149‑1 (MQ=255)
                                                                                          aGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCg                                                                                      >  5:284028/1‑122 (MQ=255)
                                                                                          aGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCg                                                                                      <  6:284028/122‑1 (MQ=255)
                                                                                          aGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCa                                                           >  4:228612/1‑149 (MQ=255)
                                                                                                 tttGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCAc                                                    >  7:339788/1‑149 (MQ=255)
                                                                                                 tttGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCAc                                                    >  3:82535/1‑149 (MQ=255)
                                                                                                 tttGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCAc                                                    <  2:124261/149‑1 (MQ=255)
                                                                                                     cAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGAt                                                <  4:324728/149‑1 (MQ=255)
                                                                                                                    gCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGACACTGCCACGGATGAACAACTTCAGCtt                                 >  2:95209/1‑149 (MQ=255)
                                                                                                                         cttcAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGgc                            >  1:219014/1‑149 (MQ=255)
                                                                                                                             aaCCAGATGACCACCATGCATGGTCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTg                                                        <  4:317705/117‑1 (MQ=255)
                                                                                                                             aaCCAGATGACCACCATGCATGGTCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTg                                                        >  3:317705/1‑117 (MQ=255)
                                                                                                                             aaCCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGgctgct                        <  4:310016/149‑1 (MQ=255)
                                                                                                                                   aTGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCCGTTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCtgg                   >  1:91951/1‑149 (MQ=255)
                                                                                                                                   aTGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCcggc                  <  8:339788/149‑1 (MQ=255)
                                                                                                                                        caccaTGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCt             >  6:190082/1‑149 (MQ=255)
                                                                                                                                        caccaTGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCt             >  7:58115/1‑149 (MQ=255)
                                                                                                                                        caccaTGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCt             >  8:180378/1‑149 (MQ=255)
                                                                                                                                                   gCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCTTCctgctgctg  <  6:304816/149‑1 (MQ=255)
                                                                                                                                                   gCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCTTCctgctgctg  <  7:180378/149‑1 (MQ=255)
                                                                                                                                                   gCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCTTCctgctgctg  <  5:118336/149‑1 (MQ=255)
                                                                                                                                                   gCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCTTCctgctgctg  <  8:58115/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGACATCGGCACGATGTACCTGTGGTTCAGTTTCATCATGTTCCTGCTCGGTGGCTCGTTTGCCATGGTGATCCGCGCTGAGCTGTTCCAGCCGGGTTTGCAGATCGTGGAGCCGGCGTTCTTCAACCAGATGACCACCATGCATGGCCTGATCATGGTGTTTGGCGCGGTGATGCCGGCC‑TTCGTCGGCCTGGCCAACTGGATGATTCCGCTGATGATCGGCGCGCCCGACATGGCACTGCCACGGATGAACAACTTCAGCTTCTGGCTGCTGCCGGCGGCCTTCCTGCTGCTG  >  NC_002947/110075‑110370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑